General information for g3781.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as NAD-dependent protein deacetylase Sirt2.
Parent gene
- The parent gene g3781 is located on chr_3: 27950973..27952260 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3781 | g3781.t1 | isoform | g3781.t1 | 27950973 | 27952260 |
| chr_3 | g3781 | g3781.t1 | exon | g3781.t1.exon1 | 27950973 | 27952177 |
| chr_3 | g3781 | g3781.t1 | cds | g3781.t1.CDS1 | 27950973 | 27952177 |
| chr_3 | g3781 | g3781.t1 | exon | g3781.t1.exon2 | 27952242 | 27952260 |
| chr_3 | g3781 | g3781.t1 | cds | g3781.t1.CDS2 | 27952242 | 27952260 |
| chr_3 | g3781 | g3781.t1 | TSS | g3781.t1 | 27952379 | 27952379 |
| chr_3 | g3781 | g3781.t1 | TTS | g3781.t1 | NA | NA |
Sequences
>g3781.t1 Gene=g3781 Length=1224
ATGGCATCAAAAGACAATGAAAAATTACCACAAAATGATGATAAAGATAAGCAATCATCT
TCTTCGAGTGAGAAAAGAGAGCAAGAACTTCCTGAATCGGACAGTTTAATGGACAAAATT
GCTCATTTTTTTTCTGACAAACTTCATTTAAGTTCAGCTGCAGAAGACGATGAGGATGAA
GGCGGTCATGTTTTGCAAAGTGCCACAATCTCTGCACTAATTGATGCATGGAAGCGTGGA
TGTTTCAAAAAAATTGTGACTATGGTTGGTGCTGGAATTAGTACTTCCTCTGGCATACCT
GATTTTAGATCCAAAAATTCCGGACTATATGCAAATTTAAGCAAATACAACCTACCATAT
CCTGAAGCTATTTTTGAGATTAACTATTTTCGTGAAAATCCTCAACCGTTTTTTAAATTA
GCCAAAGAGCTCTATCCGGGATCATTTAAACCAACTCCAAGTCATTATTTCATAAAATTG
CTTCAAGACAAAGGATTATTGCTTAGACATTATACACAAAATATCGATACCTTAGAACGA
ATAGCTGGAATTGATGAGGAAAAACTTGTGGAAGCACATGGTACATTTTATAAACACCAC
TGCATCGATTGTCAAGAAGAGTATTCAATGGAATGGGCAAAAGAAGAAATTTTTAAGGAT
AATGTACCAACGTGTACAAATTGTAATGGCGTAGTAAAACCTGATATTGTATTTTTTGGG
GAAAATTTGCCAGAGCCTTTTTATGTTCTTCCTCACAGAGATTTTAAACAATGTGATTTA
TTAATCATCATGGGAACTTCTTTAACTGTTCAACCTTTCGCCTCTTTAATTGATTTCGTA
AGTGATAGTTGTGTGAGACTTTTGATTAATAGAGAAAAAGTTGGCAAGAATAGTGGCGGT
GGATTTTTACGTTCTATGATTTTCGGTGAAGGCTTATGCTTTGACTTGCCTGGTAATCGA
CGAGATGTTGCATATGAAGGTGATTGTGATGATGGATGTTTGTATTTAGCAGATCAATTG
GGATTCGGTGATGAACTTCGAGATCTTGTTAAAAAGGAGCATGAGCGAATTGATATAGAA
CTTGGAAAAATTAAACGAAAAGTATCGACTGATGATCAAAGTAAAGATTCATCAGATGAA
AAACCAGAAGAGGAGCAAACGAAAGAGAAATTGTTGAAAAATAATGAAAATTCGAGCAAA
AATGATGTTAAAAGTTCGATTTAA
>g3781.t1 Gene=g3781 Length=407
MASKDNEKLPQNDDKDKQSSSSSEKREQELPESDSLMDKIAHFFSDKLHLSSAAEDDEDE
GGHVLQSATISALIDAWKRGCFKKIVTMVGAGISTSSGIPDFRSKNSGLYANLSKYNLPY
PEAIFEINYFRENPQPFFKLAKELYPGSFKPTPSHYFIKLLQDKGLLLRHYTQNIDTLER
IAGIDEEKLVEAHGTFYKHHCIDCQEEYSMEWAKEEIFKDNVPTCTNCNGVVKPDIVFFG
ENLPEPFYVLPHRDFKQCDLLIIMGTSLTVQPFASLIDFVSDSCVRLLINREKVGKNSGG
GFLRSMIFGEGLCFDLPGNRRDVAYEGDCDDGCLYLADQLGFGDELRDLVKKEHERIDIE
LGKIKRKVSTDDQSKDSSDEKPEEEQTKEKLLKNNENSSKNDVKSSI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g3781.t1 | CDD | cd01408 | SIRT1 | 83 | 336 | 1.44888E-142 |
| 8 | g3781.t1 | Gene3D | G3DSA:3.40.50.1220 | - | 70 | 349 | 5.7E-122 |
| 9 | g3781.t1 | Gene3D | G3DSA:3.30.1600.10 | SIR2/SIRT2 ‘Small Domain’ | 96 | 246 | 5.7E-122 |
| 7 | g3781.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 35 | - |
| 5 | g3781.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 367 | 407 | - |
| 6 | g3781.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 367 | 397 | - |
| 2 | g3781.t1 | PANTHER | PTHR11085:SF3 | NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-2 | 28 | 385 | 1.2E-132 |
| 3 | g3781.t1 | PANTHER | PTHR11085 | HISTONE DEACETYLASE SIR2 FAMILY MEMBER | 28 | 385 | 1.2E-132 |
| 1 | g3781.t1 | Pfam | PF02146 | Sir2 family | 90 | 272 | 6.5E-63 |
| 10 | g3781.t1 | ProSiteProfiles | PS50305 | Sirtuin catalytic domain profile. | 71 | 345 | 50.157 |
| 4 | g3781.t1 | SUPERFAMILY | SSF52467 | DHS-like NAD/FAD-binding domain | 69 | 356 | 3.97E-89 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0070403 | NAD+ binding | MF |
KEGG
Orthology
- This transcript has been annotated with K11412 (NAD+-dependent protein deacetylase sirtuin 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
