Gene loci information

Transcript annotation

  • This transcript has been annotated as UDP-N-acetylglucosamine–dolichyl-phosphate N-acetylglucosaminephosphotransferase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g3847 g3847.t13 TSS g3847.t13 28345844 28345844
chr_3 g3847 g3847.t13 isoform g3847.t13 28346366 28350080
chr_3 g3847 g3847.t13 exon g3847.t13.exon1 28346366 28346938
chr_3 g3847 g3847.t13 cds g3847.t13.CDS1 28346529 28346938
chr_3 g3847 g3847.t13 exon g3847.t13.exon2 28349579 28350080
chr_3 g3847 g3847.t13 cds g3847.t13.CDS2 28349579 28350080
chr_3 g3847 g3847.t13 TTS g3847.t13 28350108 28350108

Sequences

>g3847.t13 Gene=g3847 Length=1075
TCCTGAAGCATTTGGTGTGGTAACTGGTTGTGTGTTCTTAATCGCACTATTTTGCTTTAT
TCCAATACCATTTGGTCATATATTTTTTGAGGGAAAAACTGATGTGAACTTTCCACATCA
TGAATTTGTGGAATTAATTGCAGCTCTGTTATCAATTTGTTGTATGATTTTACTTGGTTT
TGCTGATGATGTACTTGATCTACGATGGCGTCATAAATTATTATTACCAACTATTGCCTC
ATTACCTCTCTTAATTGTATATTATGTGAATTTCAATTCAACAACTATAATTTTACCAAA
GATAGTTCATAACATTATATCGAAATCAATCGATATTGGATTTCTCTATTACATTTATAT
GGGAATGCTTGCAGTTTTCTGCACAAATGCTATCAATATTTTAGCAGGAATAAATGGATT
AGAAGTGGGTCAATCTATCATCATCTCAAGCTGTGTCATCATTTTTAACATGATTCAAAT
TATCAATGACTCGGTTGCAGAGAAACATCAACTCTCATTATATATTATGCTTCCTTACTT
GGCTACAACTTTAGCTTTGTGGAAATTTAACAAATACCCTTCACAAGTGTTTGTAGGTGA
CACGTTCAACTATTTATCTGGAATGACATTTGCTGTGGTTGGCATCTTAGGACACTTTAG
CAAAACAATTTTACTCTTTTTCATACCACAAATATTGAATTTCTTATATTCAGTGCCACA
ACTTTTTCATTTTATTCCATGTCCTCGTCATCGACTTCCTAAATTTGATCCCGAGACTGG
TCTTTTAAACTACAGCAAAACAACTTTTAAAGAAAATGAATTGAATTCTTTGGGACGTTT
AATATTTGTTATACTTAAAACACTGAAATTGATAAAGTGGGAAAAGAACGAAGACGACGG
TATAATTATATGCAACAATCTTACAATCATCAATTTCTTTATCCTATTTACTGGACCAAT
TCATGAAGAAAAATTGAACAACTATATTCTTGTTTTTCAAATTATTTGTTCATGTTTTGC
ATTTCTAATTCGATATCCATTGGCGCAATATTTTTATAGTGACAATCTGTCATAG

>g3847.t13 Gene=g3847 Length=303
MILLGFADDVLDLRWRHKLLLPTIASLPLLIVYYVNFNSTTIILPKIVHNIISKSIDIGF
LYYIYMGMLAVFCTNAINILAGINGLEVGQSIIISSCVIIFNMIQIINDSVAEKHQLSLY
IMLPYLATTLALWKFNKYPSQVFVGDTFNYLSGMTFAVVGILGHFSKTILLFFIPQILNF
LYSVPQLFHFIPCPRHRLPKFDPETGLLNYSKTTFKENELNSLGRLIFVILKTLKLIKWE
KNEDDGIIICNNLTIINFFILFTGPIHEEKLNNYILVFQIICSCFAFLIRYPLAQYFYSD
NLS

Protein features from InterProScan

Transcript Database ID Name Start End E.value
27 g3847.t13 CDD cd06855 GT_GPT_euk 1 211 3.8558E-106
2 g3847.t13 PANTHER PTHR10571 UDP-N-ACETYLGLUCOSAMINE–DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE 1 298 1.8E-121
1 g3847.t13 Pfam PF00953 Glycosyl transferase family 4 1 163 6.8E-36
16 g3847.t13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 19 -
20 g3847.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 20 39 -
13 g3847.t13 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 40 59 -
22 g3847.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 60 83 -
18 g3847.t13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 84 88 -
21 g3847.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 89 107 -
11 g3847.t13 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 108 118 -
23 g3847.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 119 136 -
15 g3847.t13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 137 141 -
25 g3847.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 142 162 -
12 g3847.t13 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 163 168 -
24 g3847.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 169 191 -
14 g3847.t13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 192 245 -
19 g3847.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 246 262 -
10 g3847.t13 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 263 273 -
26 g3847.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 274 293 -
17 g3847.t13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 294 303 -
6 g3847.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 20 37 -
9 g3847.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 58 80 -
5 g3847.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 85 104 -
4 g3847.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 117 136 -
7 g3847.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 151 173 -
3 g3847.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 247 266 -
8 g3847.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 276 298 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0003975 UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity MF
GO:0016021 integral component of membrane CC
GO:0006487 protein N-linked glycosylation BP
GO:0006488 dolichol-linked oligosaccharide biosynthetic process BP
GO:0008963 phospho-N-acetylmuramoyl-pentapeptide-transferase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values