General information for g3881.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as E3 SUMO-protein ligase PIAS1.
Parent gene
- The parent gene g3881 is located on chr_3: 28604776..28606316 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3881 | g3881.t3 | TSS | g3881.t3 | 28604708 | 28604708 |
| chr_3 | g3881 | g3881.t3 | isoform | g3881.t3 | 28604776 | 28606316 |
| chr_3 | g3881 | g3881.t3 | exon | g3881.t3.exon1 | 28604776 | 28604878 |
| chr_3 | g3881 | g3881.t3 | cds | g3881.t3.CDS1 | 28604816 | 28604878 |
| chr_3 | g3881 | g3881.t3 | exon | g3881.t3.exon2 | 28604939 | 28605259 |
| chr_3 | g3881 | g3881.t3 | cds | g3881.t3.CDS2 | 28604939 | 28605259 |
| chr_3 | g3881 | g3881.t3 | exon | g3881.t3.exon3 | 28605316 | 28605424 |
| chr_3 | g3881 | g3881.t3 | cds | g3881.t3.CDS3 | 28605316 | 28605424 |
| chr_3 | g3881 | g3881.t3 | exon | g3881.t3.exon4 | 28605477 | 28605554 |
| chr_3 | g3881 | g3881.t3 | cds | g3881.t3.CDS4 | 28605477 | 28605554 |
| chr_3 | g3881 | g3881.t3 | exon | g3881.t3.exon5 | 28605616 | 28605784 |
| chr_3 | g3881 | g3881.t3 | cds | g3881.t3.CDS5 | 28605616 | 28605784 |
| chr_3 | g3881 | g3881.t3 | exon | g3881.t3.exon6 | 28605843 | 28606316 |
| chr_3 | g3881 | g3881.t3 | cds | g3881.t3.CDS6 | 28605843 | 28606236 |
| chr_3 | g3881 | g3881.t3 | TTS | g3881.t3 | 28606435 | 28606435 |
Sequences
>g3881.t3 Gene=g3881 Length=1254
AATTACAAACGAACGCGAACCATTAAAATTTCCAAAAGTCATGCCAAACAATATATCTAA
ACCATATGAACTAAAAGTGAGTGATACAAACAAAACAATTCAGCAGTGTTTACCAACGGC
TTCCAATATTGTTCAACTACAAATTAAAAATCTACCTTTTTATGATAAATTAGCAGTGCT
ATTAAAACCAATTCATTTAAATCCTAATCAAACCACATCAACCCAAAAAGCTTTAATTGA
TTTAAGTTTGAAAATTGAACATATTCAAATTATTCAAAAATACCGCCGCAATGATTCTAA
CGGAACAGTTTACGATGCACAAGTGATACTGAGATTCTGTCTTTTTGATACTTCTACTAC
ACAAAACGATAATTTCCCTTTAAATCTCGACCTATTTGTTAATGGTTGTCCTTGTGAACT
TCCCAATTATTTACCATGTCGACCTGGCGATACTCCTAGACGCCCACCACAGCCCATTAA
TATAACTAAATATCTCAAAATCAATCCTGAATCATCAAATCAACTTATAAATAAAATTAT
AGTGCATTGGTCTCAAGAACCTTCAAAAAAGTATATGTTAAGTGCATTTCTAGTGCATAA
ACGAACTTCTGCTGAACTATTTGATCGGATCAAGTTAAGAGATGTAAAGCCACCAAATTA
CACAAGTACACTTATTAAAGAGAAGTTGAAAGATGATGCAGATAATGAAATTGCAACCAC
AATGTTGAGAGCTTCATTGATTTGTCCATTAGCTAAAACAAGAATGAAAACACCTTTTCG
CTCGTTAAGTTGTAAGCATGTTCAATGCTTTGATGGACTTTCATACTTGCAAATGAATGA
ACGCCATCCAAAATGGACTTGTCCAGTGTGCAATAAGGAAATTACATATGAAAGTTTAGT
AATCGATGGATATTTTCTTAGTGTTATCAAATCTATCAATGACAATGAAACAACAGAAAT
TCAATTGCATTCAAATGGATCTTGGTCGAAATTTGAAATGGAAAAAAACAACATAGTGAA
GAAAAAAGCAGCATCAAAAAATATTGATGTAATTGATGTTTGCGATAGTGACGAAGAAGA
TGCACAAAACAATAATCAACCTTGTAATTTAATTAATATTAACGTTTTGATATTTTCCAG
TATTAACTTATTTTCCTTATTCAATTTACGATAGGTTCATCAAATTCTGAGAGAAAGCTA
CTTCAACCATGTCGTGACGGAGATTATATTGATCTTACTTTAGACGACGATTGA
>g3881.t3 Gene=g3881 Length=377
MPNNISKPYELKVSDTNKTIQQCLPTASNIVQLQIKNLPFYDKLAVLLKPIHLNPNQTTS
TQKALIDLSLKIEHIQIIQKYRRNDSNGTVYDAQVILRFCLFDTSTTQNDNFPLNLDLFV
NGCPCELPNYLPCRPGDTPRRPPQPINITKYLKINPESSNQLINKIIVHWSQEPSKKYML
SAFLVHKRTSAELFDRIKLRDVKPPNYTSTLIKEKLKDDADNEIATTMLRASLICPLAKT
RMKTPFRSLSCKHVQCFDGLSYLQMNERHPKWTCPVCNKEITYESLVIDGYFLSVIKSIN
DNETTEIQLHSNGSWSKFEMEKNNIVKKKAASKNIDVIDVCDSDEEDAQNNNQPCNLINI
NVLIFSSINLFSLFNLR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g3881.t3 | Gene3D | G3DSA:2.60.120.780 | - | 32 | 187 | 2.4E-38 |
| 7 | g3881.t3 | Gene3D | G3DSA:3.30.40.10 | Zinc/RING finger domain | 189 | 317 | 4.8E-40 |
| 3 | g3881.t3 | PANTHER | PTHR10782 | ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN | 33 | 347 | 2.6E-73 |
| 4 | g3881.t3 | PANTHER | PTHR10782:SF11 | E3 SUMO-PROTEIN LIGASE PIAS1 | 33 | 347 | 2.6E-73 |
| 2 | g3881.t3 | Pfam | PF14324 | PINIT domain | 33 | 185 | 1.0E-18 |
| 1 | g3881.t3 | Pfam | PF02891 | MIZ/SP-RING zinc finger | 232 | 279 | 2.0E-21 |
| 11 | g3881.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 356 | - |
| 12 | g3881.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 357 | 376 | - |
| 10 | g3881.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 377 | 377 | - |
| 9 | g3881.t3 | ProSiteProfiles | PS51466 | PINIT domain profile. | 22 | 188 | 28.308 |
| 8 | g3881.t3 | ProSiteProfiles | PS51044 | Zinc finger SP-RING-type profile. | 220 | 297 | 41.243 |
| 5 | g3881.t3 | SUPERFAMILY | SSF57850 | RING/U-box | 230 | 298 | 2.39E-7 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008270 | zinc ion binding | MF |
| GO:0019789 | SUMO transferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04706 (E3 SUMO-protein ligase PIAS1 [EC:2.3.2.-])
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed