General information for g3914.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Aminopeptidase N.
Parent gene
- The parent gene g3914 is located on chr_3: 28856161..28860535 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3914 | g3914.t1 | TSS | g3914.t1 | 28855395 | 28855395 |
| chr_3 | g3914 | g3914.t1 | isoform | g3914.t1 | 28856161 | 28860535 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon1 | 28856161 | 28856427 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS1 | 28856161 | 28856427 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon2 | 28857054 | 28857746 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS2 | 28857054 | 28857746 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon3 | 28857806 | 28857835 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS3 | 28857806 | 28857835 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon4 | 28857897 | 28857907 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS4 | 28857897 | 28857907 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon5 | 28857976 | 28858084 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS5 | 28857976 | 28858084 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon6 | 28858163 | 28858189 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS6 | 28858163 | 28858189 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon7 | 28858257 | 28858694 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS7 | 28858257 | 28858694 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon8 | 28858799 | 28858957 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS8 | 28858799 | 28858957 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon9 | 28859105 | 28859323 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS9 | 28859105 | 28859323 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon10 | 28859385 | 28859662 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS10 | 28859385 | 28859662 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon11 | 28859722 | 28859814 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS11 | 28859722 | 28859814 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon12 | 28859869 | 28860177 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS12 | 28859869 | 28860177 |
| chr_3 | g3914 | g3914.t1 | exon | g3914.t1.exon13 | 28860229 | 28860535 |
| chr_3 | g3914 | g3914.t1 | cds | g3914.t1.CDS13 | 28860229 | 28860535 |
| chr_3 | g3914 | g3914.t1 | TTS | g3914.t1 | 28861098 | 28861098 |
Sequences
>g3914.t1 Gene=g3914 Length=2940
ATGCGTTGGTTGGAAATTGCATTATGCGTGTTCTTTATATACAACAATGTAATCACAGCA
AATCCAACTGCACAAAAGGAAGAAGAGGAAAAGCAGCCGGTAAAAGAAGAACAGCAAACA
TTGATTGCCGATGAGAATGATGAGAAGAAAGTTTATGACAATTACCGTTTGCCACTTTCA
ATCACACCAGATAATTATAAATTGGAAATTTTCACACATCTCAATGATTCTGAAGGATTT
GCATTCAAGGGCATTGTTGAAATTACGCTAACTGTGCACGAGTTTGCCGATAACATCACG
TTGCACGCAAAAAATCTGACAATACCGACAATGAATATTGAGTTACGCGATGTTACTGAA
AATGAAAATGTTAAGATTAAAAGTGTGGAGCTTATGCCTAAGCATGATTTTCTCATCATC
TATCCACAATCAATTCTATCGAAATTTAGAAAATATGTCGTGAAAATTCCATTTGTTGGC
TCACTTGATACTGACTTAGTTGGTTATTATAGAAGTTCATATTTGGATAAAGCAGCTAAT
AAAAGAATTTGGCTATCAGTTACACAATTTGAACCAATTCATGCACGTGAAGCTTTTCCA
TGCTTTGATGAGCCTGAAATGAAAGCAACATTCGATATTTCGCTTGCACATCATAAAATG
TATAATTCACTGAGCAATATGCCGCTGAAAGAGACAAAAGAAGTTGAAAGTGTGAAAGAT
TTTGTTAAGAATACATTTGAGACTTCAGTAAAAATGAGCACATACCTTGTTGCTTATTGT
GTCTCTGATTTTGAGTACAAAGAATCTGTTGTGAAAATGAAAGATGATGTAAAATTCAGA
ATTTATGCTCGGCGTGATGCAATGGAACAAGTTGATTATGCAGCTGAAATTGGACCAAAA
GTGCTGAAATTTTATGAAGATTATTTTGATATTAAATATCCTTTACCAAAAGTAGACATG
ATTGCAGTACCTGATTTTGCTGCTGGTGCTATGGAAAATTGGGGTCTCATAACATACCGT
GAAACAGCTCTTTTATATCAATCAAATGCATCAACATTATCAGCAAAATATCGAGTTGCA
GAAGTTGTTGCACATGAATTAGCACATCAGTGGTTCGGAAACCTTTGTACTATGAAGTGG
TGGAGTGATCTTTGGTTAAATGAAGGATTTGCAACATATGTCGCAAGTTTAGGTGTAGCA
CATTTGCATCCAGAATGGAAATCTTTAGATGAAGAGTCAGTGGATAACACTTTGGATATT
TTCAAAGTTGATGCCTTAAAAGCAAGTCATCCAGTCTCTGTAACAATCGGTCATCCAAAT
CAGATCTCACAAATTTTCGATGCTATTAGTTACTCAAAAGGTTCAGTTTTAATTCGCATG
ATGCACAAGTTTCTGGGTGAAAAAGCTTTCCGCTTTGGTGTTTCAAATTATTTAAAGAAA
CATGCATATGCAAATGCTGAACAAGATGACCTTTGGCACTCACTTACTGCTAGTGCTCAT
GAAGAGCAATCATTACCAACATCAATTAGTGTTAAAGATATTATGGACACTTGGACTTTA
CAAGTTGGTTATCCGGTAATTGATGTAAAGAGAGATTATGACACAAACTCAGCTACAATA
ACTCAATCAAGATATCTTTCTGATCGCTACACAACAAGAAGTGATTTAGACTTTTGCTGG
TGGATTCCTTTGACTTATACATTTGGTGAAAATAGAAATTTTACTAGTTCATCTGCACAA
GATTGGATGACATGCAATAAAAACAAAGAAACTGAAGAGAAAAAGATTGAAAATCTTCCT
GATAGTAAGCACTGGGCTATTTTCAATATTCAACTTGCTGGTCTATACAAAATAAAATAT
GATGCTGACAATTACAAGCTGATTGTTGATACTCTAAATAGTGATGAATTTGAGACCATT
CACGTTATTAATAGAGCCCAATTGATTGATGATGCAATGGATTTAGCATGGACAGGACAA
CAAGATTATGGAATTGCACTTGATATGATTAATTATTTAAAACAAGAAAGCGAGTATATT
CCATGGAAAGCAGCTCTTGATAATCTTCGTATCGTCAACCGTTTGTTGATTCGTTCGCAA
CTTTATGGAGTTTTCAAGGCTTACATTCGACACATTTTGAAACCAATTTATGCAAAAGTT
GGCGGCATTAGCAAAGTACCAGATCAAACTCAACTTGCTTCAGTTAAACATCAATCAATG
ATTTGCGCTTGGTCATGTCGATTTGATGTTGGCGATTGTGTCAAAAAGTCAGTTGAATTT
TTCAATAATTGGATGATTGAAACTGAACCTGATCATATTAATCCAATTCCATTAAATCTC
CGACCAGTCATTTATTGTACAGCAATTCGTCTTGGCAATGAAAACCACTGGAAATTTTTA
TGGCAACGATACGTTAATAGCAATGTTGGTGCAGAAAAGTCAATGATAATTTCAGCATTG
TCATGTTCTCGAGAACAATGGGTCTTAAGTCGCTATCTCGAATGGTCACTTAATTCTACT
TTGGTGAGAAAACAAGATGCAAGTTTTGTCTTTAGTGGAATTGCAAGAGAGGAAATTGGT
TTCTTTTTGGCCAAAAGCTTCTTCTTTGATCGAATTGATGATATTTATCAAACCTTGTAT
CCAGATACTTCTCGATTATCACGATATATTAAACCACTAGCTGAACAAATGTCAACACAA
AAAGAATTGACTGAATTGAAAGATTTAGTGTCAATAAAATCGAAAGCATTTGAGAAATCT
TCACAAGGCGTCAGACAAGCATTGGAGACAGTTGAATTAAATAATCAGTGGAAAATTAAC
AATTATCTTCAACTTGCGTCACGTCTCAATCATATGAGAATGAGAAATTTTGATTACGAA
GATGAAATTGCTGAGGAAAATAATGAAAAAAAATCTGCTACTGAACTCAGCATGGCATGA
>g3914.t1 Gene=g3914 Length=979
MRWLEIALCVFFIYNNVITANPTAQKEEEEKQPVKEEQQTLIADENDEKKVYDNYRLPLS
ITPDNYKLEIFTHLNDSEGFAFKGIVEITLTVHEFADNITLHAKNLTIPTMNIELRDVTE
NENVKIKSVELMPKHDFLIIYPQSILSKFRKYVVKIPFVGSLDTDLVGYYRSSYLDKAAN
KRIWLSVTQFEPIHAREAFPCFDEPEMKATFDISLAHHKMYNSLSNMPLKETKEVESVKD
FVKNTFETSVKMSTYLVAYCVSDFEYKESVVKMKDDVKFRIYARRDAMEQVDYAAEIGPK
VLKFYEDYFDIKYPLPKVDMIAVPDFAAGAMENWGLITYRETALLYQSNASTLSAKYRVA
EVVAHELAHQWFGNLCTMKWWSDLWLNEGFATYVASLGVAHLHPEWKSLDEESVDNTLDI
FKVDALKASHPVSVTIGHPNQISQIFDAISYSKGSVLIRMMHKFLGEKAFRFGVSNYLKK
HAYANAEQDDLWHSLTASAHEEQSLPTSISVKDIMDTWTLQVGYPVIDVKRDYDTNSATI
TQSRYLSDRYTTRSDLDFCWWIPLTYTFGENRNFTSSSAQDWMTCNKNKETEEKKIENLP
DSKHWAIFNIQLAGLYKIKYDADNYKLIVDTLNSDEFETIHVINRAQLIDDAMDLAWTGQ
QDYGIALDMINYLKQESEYIPWKAALDNLRIVNRLLIRSQLYGVFKAYIRHILKPIYAKV
GGISKVPDQTQLASVKHQSMICAWSCRFDVGDCVKKSVEFFNNWMIETEPDHINPIPLNL
RPVIYCTAIRLGNENHWKFLWQRYVNSNVGAEKSMIISALSCSREQWVLSRYLEWSLNST
LVRKQDASFVFSGIAREEIGFFLAKSFFFDRIDDIYQTLYPDTSRLSRYIKPLAEQMSTQ
KELTELKDLVSIKSKAFEKSSQGVRQALETVELNNQWKINNYLQLASRLNHMRMRNFDYE
DEIAEENNEKKSATELSMA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 24 | g3914.t1 | CDD | cd09601 | M1_APN-Q_like | 65 | 520 | 0.0 |
| 18 | g3914.t1 | Coils | Coil | Coil | 949 | 976 | - |
| 15 | g3914.t1 | Gene3D | G3DSA:2.60.40.1730 | tricorn interacting facor f3 domain | 47 | 264 | 4.6E-66 |
| 16 | g3914.t1 | Gene3D | G3DSA:1.10.390.60 | - | 270 | 523 | 2.1E-108 |
| 14 | g3914.t1 | Gene3D | G3DSA:2.60.40.1910 | - | 524 | 620 | 9.3E-24 |
| 17 | g3914.t1 | Gene3D | G3DSA:1.25.50.20 | - | 621 | 957 | 6.6E-98 |
| 4 | g3914.t1 | PANTHER | PTHR11533:SF253 | AMINOPEPTIDASE | 40 | 952 | 0.0 |
| 5 | g3914.t1 | PANTHER | PTHR11533 | PROTEASE M1 ZINC METALLOPROTEASE | 40 | 952 | 0.0 |
| 9 | g3914.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 196 | 211 | 2.6E-31 |
| 6 | g3914.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 246 | 261 | 2.6E-31 |
| 10 | g3914.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 326 | 336 | 2.6E-31 |
| 8 | g3914.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 362 | 377 | 2.6E-31 |
| 7 | g3914.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 381 | 393 | 2.6E-31 |
| 3 | g3914.t1 | Pfam | PF17900 | Peptidase M1 N-terminal domain | 63 | 256 | 3.2E-47 |
| 2 | g3914.t1 | Pfam | PF01433 | Peptidase family M1 domain | 293 | 518 | 3.9E-82 |
| 1 | g3914.t1 | Pfam | PF11838 | ERAP1-like C-terminal domain | 605 | 932 | 3.0E-74 |
| 20 | g3914.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 21 | g3914.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 22 | g3914.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 14 | - |
| 23 | g3914.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 19 | - |
| 19 | g3914.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 979 | - |
| 12 | g3914.t1 | SUPERFAMILY | SSF63737 | Leukotriene A4 hydrolase N-terminal domain | 52 | 263 | 1.57E-48 |
| 11 | g3914.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 275 | 529 | 2.64E-86 |
| 13 | g3914.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K11140 (aminopeptidase N)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
