General information for g4012.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Acrosin.
Parent gene
- The parent gene g4012 is located on chr_3: 29516275..29517043 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g4012 | g4012.t1 | isoform | g4012.t1 | 29515456 | 29517043 |
| chr_3 | g4012 | g4012.t1 | exon | g4012.t1.exon1 | 29515456 | 29516275 |
| chr_3 | g4012 | g4012.t1 | cds | g4012.t1.CDS1 | 29515456 | 29516275 |
| chr_3 | g4012 | g4012.t1 | exon | g4012.t1.exon2 | 29516362 | 29516451 |
| chr_3 | g4012 | g4012.t1 | cds | g4012.t1.CDS2 | 29516362 | 29516451 |
| chr_3 | g4012 | g4012.t1 | exon | g4012.t1.exon3 | 29516515 | 29516656 |
| chr_3 | g4012 | g4012.t1 | cds | g4012.t1.CDS3 | 29516515 | 29516656 |
| chr_3 | g4012 | g4012.t1 | exon | g4012.t1.exon4 | 29516716 | 29516757 |
| chr_3 | g4012 | g4012.t1 | cds | g4012.t1.CDS4 | 29516716 | 29516757 |
| chr_3 | g4012 | g4012.t1 | exon | g4012.t1.exon5 | 29516818 | 29517043 |
| chr_3 | g4012 | g4012.t1 | cds | g4012.t1.CDS5 | 29516818 | 29517043 |
| chr_3 | g4012 | g4012.t1 | TSS | g4012.t1 | 29517808 | 29517808 |
| chr_3 | g4012 | g4012.t1 | TTS | g4012.t1 | NA | NA |
Sequences
>g4012.t1 Gene=g4012 Length=1320
ATGCCACGTGCTCTAGCAATTCCTACTGTCAATCAAGTTTGTCATTTAGCTGGTTGGGGA
GTCACTCATGAAATTCGAGTTCGTCCTCATCCAATTTTACTTCGAGTTGAGCTAGATATT
ATAAGCATAGAATTCTGCAATGGAACGAATTCTTATAATGGAAATATTCCTACATTTTCT
TTCTGTGCAGGAACAGTTGATGGCTCAAGAGACGCATGTTTCGGAGACTCAGGCGGAGGT
TTGATTTGTAATAATCAAATTGCTGGAGTTGTTTCATTTGGCTTTGGCTGTGGAAGAAGG
AATTTTCCTGGAGTTTACGTTGCTGTTTCACCATTTAATTTATGGATTCAAGAATGCATA
AACTCAAATCTTCCTCATGAAGAAATTCCTAGACCGCCAGTTAGACCTAGCTCTGTTTCA
GCAAATTCACCATTGAAAATTCAGTTGCTTATCTTAGTCACATTTGCGATAGTAAAACTC
TCTCGAAATTTAAACAACACTAATCAAATTACTTCATTTAGTTGCATTCTCGTTAATCAA
AATTTCCTGAATTATTTATTCTTCAATTCCAAAAAAATGGAAGAAGATTTTGAAAAACTT
TCAATTGAAAAATATAATAAACAGCAAAAATTACTTGAGAAAATTGCAGCCTCATTAAAA
CATCAAGATGAAAAAAAATTAGCAAGAGATTCTTTAGAAACACTTTCAGTATTATCAAAT
CATAGTACAACACCATATACAAAAACCATCAGTCAAACAATTGCAGAATTGAAGCAGTAT
ACTAAAGATCAACAACAAAAATCAAAAGAAATTGATGAGAAACTTGAGAAAATTCACGAA
ACGCTTGAAAATTTTGATAATATTAATATTAAAATTAAACCAGAAGAGAAGAATATTGAA
GCTAGTGATTCAAAAACAGTTTCATATAAAACTCGTGTGCATCAGATAAAAGTTGAAGGT
TTAAATCATGAAGAAAGTTCTTCTGAATTATGGGTGCCTTATTCAACAGCTAAAAAAATT
AAACCACCAACTATAAGAGAAACAAAGGCTTCAAAATTGAAGACAAACTTCTTAAAAAAT
AAATTTTTTGATGATCAAAATTCTCAATTAAAATCAGAAACTTTAAAATCAAAATTGAAT
AGCGTGAAGAAACCAACTAATAAAATTGAAACGCCAAAAGTTAATAAGAAAGTTCAAAAT
GTGAACCAATCAGTCAAAAAAATTCTTGATAATGCAAAAGAAAAACACAAGAATAATTAT
TTACAAAACAATATGAAAACTGTTTCATTAAATGTTCAAAATTTACAATTGAGTGGTTGA
>g4012.t1 Gene=g4012 Length=439
MPRALAIPTVNQVCHLAGWGVTHEIRVRPHPILLRVELDIISIEFCNGTNSYNGNIPTFS
FCAGTVDGSRDACFGDSGGGLICNNQIAGVVSFGFGCGRRNFPGVYVAVSPFNLWIQECI
NSNLPHEEIPRPPVRPSSVSANSPLKIQLLILVTFAIVKLSRNLNNTNQITSFSCILVNQ
NFLNYLFFNSKKMEEDFEKLSIEKYNKQQKLLEKIAASLKHQDEKKLARDSLETLSVLSN
HSTTPYTKTISQTIAELKQYTKDQQQKSKEIDEKLEKIHETLENFDNINIKIKPEEKNIE
ASDSKTVSYKTRVHQIKVEGLNHEESSSELWVPYSTAKKIKPPTIRETKASKLKTNFLKN
KFFDDQNSQLKSETLKSKLNSVKKPTNKIETPKVNKKVQNVNQSVKKILDNAKEKHKNNY
LQNNMKTVSLNVQNLQLSG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g4012.t1 | CDD | cd00190 | Tryp_SPc | 2 | 118 | 1.35732E-35 |
| 6 | g4012.t1 | Coils | Coil | Coil | 202 | 222 | - |
| 8 | g4012.t1 | Coils | Coil | Coil | 257 | 277 | - |
| 7 | g4012.t1 | Coils | Coil | Coil | 395 | 415 | - |
| 5 | g4012.t1 | Gene3D | G3DSA:2.40.10.10 | - | 8 | 110 | 5.8E-30 |
| 2 | g4012.t1 | PANTHER | PTHR24264 | TRYPSIN-RELATED | 5 | 123 | 8.5E-31 |
| 3 | g4012.t1 | PANTHER | PTHR24264:SF20 | ZMP:0000001088 | 5 | 123 | 8.5E-31 |
| 1 | g4012.t1 | Pfam | PF00089 | Trypsin | 6 | 116 | 4.0E-24 |
| 11 | g4012.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 1 | 121 | 16.491 |
| 10 | g4012.t1 | SMART | SM00020 | trypsin_2 | 1 | 116 | 1.7E-4 |
| 4 | g4012.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 7 | 125 | 4.57E-33 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed