General information for g4101.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Sterol O-acyltransferase 1.
Parent gene
- The parent gene g4101 is located on chr_3: 30582564..30583724 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g4101 | g4101.t1 | TTS | g4101.t1 | 30581898 | 30581898 |
| chr_3 | g4101 | g4101.t1 | isoform | g4101.t1 | 30581961 | 30583724 |
| chr_3 | g4101 | g4101.t1 | exon | g4101.t1.exon1 | 30581961 | 30582488 |
| chr_3 | g4101 | g4101.t1 | cds | g4101.t1.CDS1 | 30581961 | 30582488 |
| chr_3 | g4101 | g4101.t1 | exon | g4101.t1.exon2 | 30582546 | 30583724 |
| chr_3 | g4101 | g4101.t1 | cds | g4101.t1.CDS2 | 30582546 | 30583724 |
| chr_3 | g4101 | g4101.t1 | TSS | g4101.t1 | 30583773 | 30583773 |
Sequences
>g4101.t1 Gene=g4101 Length=1707
ATGGCAGAAAAAAGAGTTCGTCGTTCAAGTTCAATTATTCAAGATGTCGAGTTAGAAGTG
TTAAAAGAAAACTTAAGAAACAAGCACAAACAGATTGTCGAAGAAGTTGATGAAAAAATT
GATAGAATTTTTGAAGAATTTTTTCATGAAGTTAATAAACTAAGTCTTAATCGATTTGAA
GTTGAAAAGTTATTCAGTGATGGACAAGCAAATAAAAGTAAAAATATAAAACAAAAGAAA
CAAACCAAAAGAATCACGAAAAATGATCAAAACTTACCGGATAAATTACCAGAAAAACAT
TTTCAACCGCGCAATTCATTACTCACTGATCTCTTCGAAATTAAACACGTCAAGACAATT
TATCATATTTTTATCGTTATCTTTAACCTTCTACTTCTAAATGTATTTGTGTCCGATTTC
GTAGATACAGGAAAAATTAATATTGGTTTAGATCCGATTATAACGGGTTTTAAAGGTTTT
AAATATGCCATTTTTATCTGGTGCTGGATGCAATTAACAGTTTTTGGTCTCTATCCTGTT
TTTACAACATGGGCTGCCATGACAAGAAAATATTTTTCAAAGCGAGGATTTCTCACAACA
ATCTGGCATTATTGCGGAGTTATTTCAGTTATTGCATATATTTTTGGCTTCATTACCATT
TACACAAAAGCAGTTGTTCATTTCAATTTAGGACAAGCTAGTTCAGTAGCTGTTTTGATG
GAACTTGTGAGATTTGTAATGAAAGCTTATGCATTTATTCGTACAAATGTACCAAGAGCA
TTAAAATTAGGCAGAAAACGTAAATCATCAGAGGAAAATTCATCATCAGACGATAGCATA
TATGATTCAACATTACCAGCAAGTGGAAGCGTTAAAGTCAATGAAAAACAACAGCTGCCA
TACTTTAGGCAATATGTGTACTTCCTATTTGCACCAACACTCATCTATCGTGATGAATAT
CCAAGAAATAAATTAATTCGATGGAATTTTATTCTTGTTTGTTTTCTCGAAGTTGCGTCA
GTAATTTTCATCATGTCATTCATTTCTGAGCGATTCATGTATCCACTATTTCATGCATTT
GGTTCACAATTTTATGAAGTTGGAAGTAAAGAATTGATGGGATCAATTTTTAATAGCATG
TTACCTGGTCTTTTATGTTTCCTTATTGGATTTTATTGTGTACTTCATTCATGGATGAAT
GCATTTGCAGAGTTCCTTAGATTTTCTGATCGACAATTTTATCGCGATTGGTGGAATAGT
ACAAATTTTTCAGTTTATTATCGTACATGGAATGTAATTGTGCATGATTGGCTTTATCAG
TATGTCTACAGAGACCTTTATTTGCACATTTTCAAAAAGAAGCGTGGAATTTGCCAGTTT
TTAGTATTCTGCTTATCAGCAATCTTTCATGAATACATTCTTGGTTTTACCTTCAGATTT
TGTTATCCAATTTTATTCGTAGAATTTCAAGGTGCTGGTGTATTGTTGTTCTTTGCGACA
AGAAAAGATATGAAAACAATTGGAAATTGCTTCATGTGGATGAGTATTGCTATAGGAATG
GGAATATTGCTAAGCTTATATCACATGGAATATTATGCACGCCAGAATTGCTCATGGGAC
AAAGATAAAATTGTCAACTATTTTATTCCTATAAGTTGGAGTTGTAATGGATTAAAAATC
AGTGAAAATTGGAAGATTCAATTTTAA
>g4101.t1 Gene=g4101 Length=568
MAEKRVRRSSSIIQDVELEVLKENLRNKHKQIVEEVDEKIDRIFEEFFHEVNKLSLNRFE
VEKLFSDGQANKSKNIKQKKQTKRITKNDQNLPDKLPEKHFQPRNSLLTDLFEIKHVKTI
YHIFIVIFNLLLLNVFVSDFVDTGKINIGLDPIITGFKGFKYAIFIWCWMQLTVFGLYPV
FTTWAAMTRKYFSKRGFLTTIWHYCGVISVIAYIFGFITIYTKAVVHFNLGQASSVAVLM
ELVRFVMKAYAFIRTNVPRALKLGRKRKSSEENSSSDDSIYDSTLPASGSVKVNEKQQLP
YFRQYVYFLFAPTLIYRDEYPRNKLIRWNFILVCFLEVASVIFIMSFISERFMYPLFHAF
GSQFYEVGSKELMGSIFNSMLPGLLCFLIGFYCVLHSWMNAFAEFLRFSDRQFYRDWWNS
TNFSVYYRTWNVIVHDWLYQYVYRDLYLHIFKKKRGICQFLVFCLSAIFHEYILGFTFRF
CYPILFVEFQGAGVLLFFATRKDMKTIGNCFMWMSIAIGMGILLSLYHMEYYARQNCSWD
KDKIVNYFIPISWSCNGLKISENWKIQF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g4101.t1 | Coils | Coil | Coil | 18 | 42 | - |
| 2 | g4101.t1 | PANTHER | PTHR10408:SF8 | O-ACYLTRANSFERASE | 70 | 548 | 4.6E-153 |
| 3 | g4101.t1 | PANTHER | PTHR10408 | STEROL O-ACYLTRANSFERASE | 70 | 548 | 4.6E-153 |
| 33 | g4101.t1 | PIRSF | PIRSF000439 | Oat_ACAT_DAG_ARE | 2 | 539 | 2.3E-106 |
| 1 | g4101.t1 | Pfam | PF03062 | MBOAT, membrane-bound O-acyltransferase family | 295 | 527 | 6.8E-26 |
| 17 | g4101.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 119 | - |
| 28 | g4101.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 120 | 140 | - |
| 20 | g4101.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 141 | 159 | - |
| 26 | g4101.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 160 | 181 | - |
| 16 | g4101.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 182 | 200 | - |
| 24 | g4101.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 201 | 221 | - |
| 19 | g4101.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 222 | 232 | - |
| 31 | g4101.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 233 | 253 | - |
| 15 | g4101.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 254 | 324 | - |
| 27 | g4101.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 325 | 348 | - |
| 23 | g4101.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 349 | 379 | - |
| 32 | g4101.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 380 | 403 | - |
| 18 | g4101.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 404 | 455 | - |
| 29 | g4101.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 456 | 474 | - |
| 22 | g4101.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 475 | 479 | - |
| 30 | g4101.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 480 | 499 | - |
| 14 | g4101.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 500 | 510 | - |
| 25 | g4101.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 511 | 529 | - |
| 21 | g4101.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 530 | 568 | - |
| 7 | g4101.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 119 | 141 | - |
| 12 | g4101.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 163 | 185 | - |
| 9 | g4101.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 198 | 220 | - |
| 6 | g4101.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 224 | 246 | - |
| 11 | g4101.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 326 | 348 | - |
| 5 | g4101.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 376 | 398 | - |
| 10 | g4101.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 457 | 474 | - |
| 8 | g4101.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 478 | 500 | - |
| 4 | g4101.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 507 | 529 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008374 | O-acyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00637 (sterol O-acyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed