General information for g4139.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Hormone-sensitive lipase.
Parent gene
- The parent gene g4139 is located on chr_3: 30903266..30903657 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g4139 | g4139.t1 | TTS | g4139.t1 | 30899861 | 30899861 |
| chr_3 | g4139 | g4139.t1 | isoform | g4139.t1 | 30900210 | 30903266 |
| chr_3 | g4139 | g4139.t1 | exon | g4139.t1.exon1 | 30900210 | 30900317 |
| chr_3 | g4139 | g4139.t1 | cds | g4139.t1.CDS1 | 30900210 | 30900317 |
| chr_3 | g4139 | g4139.t1 | exon | g4139.t1.exon2 | 30900375 | 30901822 |
| chr_3 | g4139 | g4139.t1 | cds | g4139.t1.CDS2 | 30900375 | 30901822 |
| chr_3 | g4139 | g4139.t1 | exon | g4139.t1.exon3 | 30901896 | 30902172 |
| chr_3 | g4139 | g4139.t1 | cds | g4139.t1.CDS3 | 30901896 | 30902172 |
| chr_3 | g4139 | g4139.t1 | exon | g4139.t1.exon4 | 30902227 | 30902611 |
| chr_3 | g4139 | g4139.t1 | cds | g4139.t1.CDS4 | 30902227 | 30902611 |
| chr_3 | g4139 | g4139.t1 | exon | g4139.t1.exon5 | 30902923 | 30903266 |
| chr_3 | g4139 | g4139.t1 | cds | g4139.t1.CDS5 | 30902923 | 30903266 |
| chr_3 | g4139 | g4139.t1 | TSS | g4139.t1 | 30903663 | 30903663 |
Sequences
>g4139.t1 Gene=g4139 Length=2562
ATGGAAAAGCATGATAAGGCAACAGAAAATTTCATTATATGGGAAGCATTAAAATCGATT
ATTATTAATAATATTGATTATTACAAAAATGATGAGACGACAAATGGATTAAAATTGCAT
GACACATTATTAGAAATAATAGATCAAATTGATGAAGCACGTCCACTTTTCGAAGAAGTT
GAATCTTTTGCCTCTGAATATGATTTAGATGAAAATACACAAGCAAATGGATATCGGAGC
TTTCTGAAAGTTTTCGATTCAGCAATCAATCATACATTAAAAATTATCAAATATGTGACA
GAGAATCGATCGGGTCTTTTGTTCCGTAAAACTGCTTATACAAAGGAAATTGAAGCATGC
AATCACTTGTTGGCCGCTCTGATTACAACATTTCATCAATTAAAACACTTTCACTTATCA
AGCAAAGAGAGAGGAAATTTGTTTCCTGAGCATTCTCCTGATGAAATTTTACAACGTGCT
CAAATGATTGATCAATTTGCATTCTATGGACGATGTTTAGGATTTCAATTTTATAATTCA
GTCAAGGCTGTATTAAAATTTATTTCTATTTCAATGGCATGCTATTCTGAGTCTTATTAT
AGTAACAATGGAAAGTTTATTAAAGCTACAAATTCAATGTTCACTAGTGGAAAATATTAC
TTTGATCCTGAAATTCGCGCGCGAAGAGTTGTTAATTTATCTCAACACGCTTCAGTCGAT
TTTTGCAAGAGTTTTTGGCAACTGGCTGATTTAGATATTGTTTCACATTCAATTCCATTT
GTGAATTATAATACAAAAGTCAATCGTCTTTTTAAAATCAACGCTGAGCCTTTGAAAATT
TCAAAACTAGATGGAGAAAGTATTGAAGTAAATGCACCGGGAACAATTCAACTGAGATTA
ATTAGTTCACATAAACGAGAGGGTATGATAATTGGTGATAAGATAAAGAATAAAGTTTCG
GACCAAATATTGCCTAAATCGAAAGCTCTTATACTACATGCACACGGTGGTGGATTTGTA
GCACAAAGTTCAAAATCTCATTTAGTATATTTACTTGAGTGGGCAGTAAATTTAAATGTT
CCAATACTATCGCTTGATTATAGTCTTTCTCCAGAGTTTCCTTATCCTCGACCATTAGAA
GAAATGTTTTACGCATATTGTTGGGTGTTAAATAATGCTGATAAACTTGGAAGTACAATA
GAAAAAATAATATTTTGTGGAGATTCAGCTGGAGCAAACTTATGTTTAGCTACTTTACTA
AAGGCAATAGATATGAAAATTCGAAAGCCCGATGGACTTTTTCTTGCTTATTGCCCTATT
CTCGTTGCCTTCGACCCTTCACCATCCAGACTATTATGTTTAATGGATCCTTTAATTCCT
ATGGGGTTCTTAATACGATGCTTAAAGGCTTATAGCAATGCTCAATCGATAGAAAACAAT
AATAATGAAGATGCTACTAGTGTTGCATCTAATCTTCAGCAATTAGAAGAAATAAAACGT
GCCAAAGTCGAACATAATGAGGAATTAAAACGGTCTTCATTAAATGTAGATATAGAACAT
GAATTCAGTGAAAATTCGGAAAAATCAGATTCATTTGAGGAAATTTCATGCTTCGAAAGA
CATCAAACTGATTCAGATATTAAAGCTCATATTTCTCAAGTAAGTGATATAGCGAGCAAT
GATACACTTGCTGGCACTTCATTTCTCACAGGAACAGATTTTAAAAACAGCAGAGATACA
ATTGAAGTTGTCAGTCCTATTGATAGTATTCAATCAGCTACGTCACTCGAAGAAGATTCT
CTACCAATAACAATTCATAAAAATGAAAGGACGACTAGTGACGAAATGGCTGATATTGAT
TTGCAGCCGACTACTTCGGAAGATACCACAATTAAAAATAGCAATAATCAGTTTGTTGAT
GAGTTTACGGAAAAATACAAATATATACTTGATACACAAGCCCATGATGATGGCAAAATA
AAACCAGTTTTGCGAAAAGTGTCACGAACGAAAAGTGAAGAGAATATTATTTTCGATGTA
TCGAGAGATACAATAAGTGTTCAAACAATTCAACAAAAAGTGCATCAGGTTGCTTCAACA
TTAGTGCATGCATGTTCAACAACACTCGATCAAATCACAAAAACTAATCGTCCAATTATA
AGAAATTATTCGTGCGATGATGCTGATGAATTTCAAATGCTTGATAATGAAACTGTACCA
CCATCGCTTTCAAATGAATTTCTCTTTACTGTTTCAAAAGACCCTTTTTTGTCGCCTTAT
TATGCTGATGATGAAATGCTCAAGCAATTTCCACCAATAAGATTTTTATCACTTACACTT
GATGCCTGTTTGGACGATTCAATTATGTATGCAAAAAGACTTAAGAAGTTGAATGTCGAT
TATAAATTAGACATTTTAGATGGGCTTCCTCATGGATTTTTAAATTTTTCTAGACTATCA
AAAGATGCACATGAAGGATCAAAAATTGCTATGAAAAGAATTGCTGAATTGTTAAACATT
ACATTGGATGATGAAGACACCAAAGTTAAATATTTTTGTTAA
>g4139.t1 Gene=g4139 Length=853
MEKHDKATENFIIWEALKSIIINNIDYYKNDETTNGLKLHDTLLEIIDQIDEARPLFEEV
ESFASEYDLDENTQANGYRSFLKVFDSAINHTLKIIKYVTENRSGLLFRKTAYTKEIEAC
NHLLAALITTFHQLKHFHLSSKERGNLFPEHSPDEILQRAQMIDQFAFYGRCLGFQFYNS
VKAVLKFISISMACYSESYYSNNGKFIKATNSMFTSGKYYFDPEIRARRVVNLSQHASVD
FCKSFWQLADLDIVSHSIPFVNYNTKVNRLFKINAEPLKISKLDGESIEVNAPGTIQLRL
ISSHKREGMIIGDKIKNKVSDQILPKSKALILHAHGGGFVAQSSKSHLVYLLEWAVNLNV
PILSLDYSLSPEFPYPRPLEEMFYAYCWVLNNADKLGSTIEKIIFCGDSAGANLCLATLL
KAIDMKIRKPDGLFLAYCPILVAFDPSPSRLLCLMDPLIPMGFLIRCLKAYSNAQSIENN
NNEDATSVASNLQQLEEIKRAKVEHNEELKRSSLNVDIEHEFSENSEKSDSFEEISCFER
HQTDSDIKAHISQVSDIASNDTLAGTSFLTGTDFKNSRDTIEVVSPIDSIQSATSLEEDS
LPITIHKNERTTSDEMADIDLQPTTSEDTTIKNSNNQFVDEFTEKYKYILDTQAHDDGKI
KPVLRKVSRTKSEENIIFDVSRDTISVQTIQQKVHQVASTLVHACSTTLDQITKTNRPII
RNYSCDDADEFQMLDNETVPPSLSNEFLFTVSKDPFLSPYYADDEMLKQFPPIRFLSLTL
DACLDDSIMYAKRLKKLNVDYKLDILDGLPHGFLNFSRLSKDAHEGSKIAMKRIAELLNI
TLDDEDTKVKYFC
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g4139.t1 | Coils | Coil | Coil | 488 | 515 | - |
| 8 | g4139.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 262 | 507 | 2.3E-35 |
| 7 | g4139.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 701 | 848 | 1.6E-15 |
| 4 | g4139.t1 | PANTHER | PTHR23025 | TRIACYLGLYCEROL LIPASE | 15 | 838 | 3.4E-171 |
| 5 | g4139.t1 | PANTHER | PTHR23025:SF3 | HORMONE-SENSITIVE LIPASE | 15 | 838 | 3.4E-171 |
| 1 | g4139.t1 | Pfam | PF06350 | Hormone-sensitive lipase (HSL) N-terminus | 14 | 309 | 8.0E-98 |
| 3 | g4139.t1 | Pfam | PF07859 | alpha/beta hydrolase fold | 331 | 442 | 9.4E-25 |
| 2 | g4139.t1 | Pfam | PF07859 | alpha/beta hydrolase fold | 753 | 814 | 2.8E-6 |
| 6 | g4139.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 328 | 836 | 4.24E-42 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016042 | lipid catabolic process | BP |
| GO:0016787 | hydrolase activity | MF |
| GO:0008203 | cholesterol metabolic process | BP |
| GO:0016298 | lipase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K07188 (hormone-sensitive lipase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
