General information for g4139.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Hormone-sensitive lipase.
Parent gene
- The parent gene g4139 is located on chr_3: 30903266..30903657 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g4139 | g4139.t2 | TTS | g4139.t2 | 30899861 | 30899861 |
| chr_3 | g4139 | g4139.t2 | isoform | g4139.t2 | 30900210 | 30902172 |
| chr_3 | g4139 | g4139.t2 | exon | g4139.t2.exon1 | 30900210 | 30901822 |
| chr_3 | g4139 | g4139.t2 | cds | g4139.t2.CDS1 | 30900210 | 30901822 |
| chr_3 | g4139 | g4139.t2 | exon | g4139.t2.exon2 | 30901896 | 30902172 |
| chr_3 | g4139 | g4139.t2 | cds | g4139.t2.CDS2 | 30901896 | 30901977 |
| chr_3 | g4139 | g4139.t2 | TSS | g4139.t2 | NA | NA |
Sequences
>g4139.t2 Gene=g4139 Length=1890
AGTTTTTGGCAACTGGCTGATTTAGATATTGTTTCACATTCAATTCCATTTGTGAATTAT
AATACAAAAGTCAATCGTCTTTTTAAAATCAACGCTGAGCCTTTGAAAATTTCAAAACTA
GATGGAGAAAGTATTGAAGTAAATGCACCGGGAACAATTCAACTGAGATTAATTAGTTCA
CATAAACGAGAGGGTATGATAATTGGTGATAAGATAAAGAATAAAGTTTCGGACCAAATA
TTGCCTAAATCGAAAGCTCTTATACTACATGCACACGGTGGTGGATTTGTAGCACAAAGT
TCAAAATCTCATTTAGTATATTTACTTGAGTGGGCAGTAAATTTAAATGTTCCAATACTA
TCGCTTGATTATAGTCTTTCTCCAGAGTTTCCTTATCCTCGACCATTAGAAGAAATGTTT
TACGCATATTGTTGGGTGTTAAATAATGCTGATAAACTTGGAAGTACAATAGAAAAAATA
ATATTTTGTGGAGATTCAGCTGGAGCAAACTTATGTTTAGCTACTTTACTAAAGGCAATA
GATATGAAAATTCGAAAGCCCGATGGACTTTTTCTTGCTTATTGCCCTATTCTCGTTGCC
TTCGACCCTTCACCATCCAGACTATTATGTTTAATGGATCCTTTAATTCCTATGGGGTTC
TTAATACGATGCTTAAAGGCTTATAGCAATGCTCAATCGATAGAAAACAATAATAATGAA
GATGCTACTAGTGTTGCATCTAATCTTCAGCAATTAGAAGAAATAAAACGTGCCAAAGTC
GAACATAATGAGGAATTAAAACGGTCTTCATTAAATGTAGATATAGAACATGAATTCAGT
GAAAATTCGGAAAAATCAGATTCATTTGAGGAAATTTCATGCTTCGAAAGACATCAAACT
GATTCAGATATTAAAGCTCATATTTCTCAAGTAAGTGATATAGCGAGCAATGATACACTT
GCTGGCACTTCATTTCTCACAGGAACAGATTTTAAAAACAGCAGAGATACAATTGAAGTT
GTCAGTCCTATTGATAGTATTCAATCAGCTACGTCACTCGAAGAAGATTCTCTACCAATA
ACAATTCATAAAAATGAAAGGACGACTAGTGACGAAATGGCTGATATTGATTTGCAGCCG
ACTACTTCGGAAGATACCACAATTAAAAATAGCAATAATCAGTTTGTTGATGAGTTTACG
GAAAAATACAAATATATACTTGATACACAAGCCCATGATGATGGCAAAATAAAACCAGTT
TTGCGAAAAGTGTCACGAACGAAAAGTGAAGAGAATATTATTTTCGATGTATCGAGAGAT
ACAATAAGTGTTCAAACAATTCAACAAAAAGTGCATCAGGTTGCTTCAACATTAGTGCAT
GCATGTTCAACAACACTCGATCAAATCACAAAAACTAATCGTCCAATTATAAGAAATTAT
TCGTGCGATGATGCTGATGAATTTCAAATGCTTGATAATGAAACTGTACCACCATCGCTT
TCAAATGAATTTCTCTTTACTGTTTCAAAAGACCCTTTTTTGTCGCCTTATTATGCTGAT
GATGAAATGCTCAAGCAATTTCCACCAATAAGATTTTTATCACTTACACTTGATGCCTGT
TTGGACGATTCAATTATGTATGCAAAAAGACTTAAGAAGTTGAATGTCGATTATAAATTA
GACATTTTAGATGGGCTTCCTCATGGATTTTTAAATTTTTCTAGAGTAAGTTTGACTTTT
ATGAATTTTTATTATTATGAGTGTCATGTTATTTTTATGCAGCTATCAAAAGATGCACAT
GAAGGATCAAAAATTGCTATGAAAAGAATTGCTGAATTGTTAAACATTACATTGGATGAT
GAAGACACCAAAGTTAAATATTTTTGTTAA
>g4139.t2 Gene=g4139 Length=564
MIIGDKIKNKVSDQILPKSKALILHAHGGGFVAQSSKSHLVYLLEWAVNLNVPILSLDYS
LSPEFPYPRPLEEMFYAYCWVLNNADKLGSTIEKIIFCGDSAGANLCLATLLKAIDMKIR
KPDGLFLAYCPILVAFDPSPSRLLCLMDPLIPMGFLIRCLKAYSNAQSIENNNNEDATSV
ASNLQQLEEIKRAKVEHNEELKRSSLNVDIEHEFSENSEKSDSFEEISCFERHQTDSDIK
AHISQVSDIASNDTLAGTSFLTGTDFKNSRDTIEVVSPIDSIQSATSLEEDSLPITIHKN
ERTTSDEMADIDLQPTTSEDTTIKNSNNQFVDEFTEKYKYILDTQAHDDGKIKPVLRKVS
RTKSEENIIFDVSRDTISVQTIQQKVHQVASTLVHACSTTLDQITKTNRPIIRNYSCDDA
DEFQMLDNETVPPSLSNEFLFTVSKDPFLSPYYADDEMLKQFPPIRFLSLTLDACLDDSI
MYAKRLKKLNVDYKLDILDGLPHGFLNFSRVSLTFMNFYYYECHVIFMQLSKDAHEGSKI
AMKRIAELLNITLDDEDTKVKYFC
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g4139.t2 | Coils | Coil | Coil | 180 | 207 | - |
| 6 | g4139.t2 | Gene3D | G3DSA:3.40.50.1820 | - | 10 | 199 | 4.2E-35 |
| 7 | g4139.t2 | Gene3D | G3DSA:3.40.50.1820 | - | 387 | 514 | 1.9E-14 |
| 3 | g4139.t2 | PANTHER | PTHR23025 | TRIACYLGLYCEROL LIPASE | 15 | 549 | 1.2E-102 |
| 4 | g4139.t2 | PANTHER | PTHR23025:SF3 | HORMONE-SENSITIVE LIPASE | 15 | 549 | 1.2E-102 |
| 1 | g4139.t2 | Pfam | PF07859 | alpha/beta hydrolase fold | 23 | 134 | 4.8E-25 |
| 2 | g4139.t2 | Pfam | PF07859 | alpha/beta hydrolase fold | 445 | 506 | 1.5E-6 |
| 5 | g4139.t2 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 20 | 510 | 4.36E-42 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
