General information for g421.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as L-threonine 3-dehydrogenase, mitochondrial.
Parent gene
- The parent gene g421 is located on chr_3: 3301722..3305517 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g421 | g421.t2 | TTS | g421.t2 | 3301618 | 3301618 |
| chr_3 | g421 | g421.t2 | isoform | g421.t2 | 3301722 | 3305517 |
| chr_3 | g421 | g421.t2 | exon | g421.t2.exon1 | 3301722 | 3301861 |
| chr_3 | g421 | g421.t2 | cds | g421.t2.CDS1 | 3301722 | 3301861 |
| chr_3 | g421 | g421.t2 | exon | g421.t2.exon2 | 3301920 | 3302074 |
| chr_3 | g421 | g421.t2 | cds | g421.t2.CDS2 | 3301920 | 3302074 |
| chr_3 | g421 | g421.t2 | exon | g421.t2.exon3 | 3302488 | 3302608 |
| chr_3 | g421 | g421.t2 | cds | g421.t2.CDS3 | 3302488 | 3302608 |
| chr_3 | g421 | g421.t2 | exon | g421.t2.exon4 | 3302676 | 3302794 |
| chr_3 | g421 | g421.t2 | cds | g421.t2.CDS4 | 3302676 | 3302794 |
| chr_3 | g421 | g421.t2 | exon | g421.t2.exon5 | 3302849 | 3303239 |
| chr_3 | g421 | g421.t2 | cds | g421.t2.CDS5 | 3302849 | 3302973 |
| chr_3 | g421 | g421.t2 | exon | g421.t2.exon6 | 3305431 | 3305517 |
| chr_3 | g421 | g421.t2 | TSS | g421.t2 | NA | NA |
Sequences
>g421.t2 Gene=g421 Length=1013
GCGAGAGTAGAGAGTAGGTATTGAATGTTTTATAATATTTATATAAAAGGAGCATCTCAT
ATGATGAAGATCATAGAATCTGCGAAGGTGGTTTGGGTCAACTCGGGACACAATGTGCAA
AGCTCTTAAGAGCAAAATATGGAAGAGACAATGTCATATTGTCTGACATTGTTAAACCTA
GTGATGAAATAATTGATGCCGGACCCTACCTCTTTGCTGATATTTTAGACTTTAAAGGTC
TTCAAAAAGCAGTTGTTTCTCATCGCATCGATTGGATAATTCATTTCTCAGCTTTACTTA
GTGCAATTGGTGAACAAAATGTGTCACTTGCTATTCGAGTAAATATAGAAGGCATGCATA
ATATGGTTGAGTTAGCAAAGCAATATAAACTGAGAATTTTCGTACCTTCAACAATTGGTG
CTTTCGGTCCAGATTCTCCTCGAAATCCTACGCCCAATTTGACTATTCAGAGGCCAAGAA
CAATCTATGGAGTTTCAAAAGTTCATGCAGAATTGATGGGCGAGTATTATCATCACAAAT
TTGGAACTGATTTTCGTTGTTTGAGATTTCCTGGTGTAATTAGTGCTGATCCTCCTGGTG
GTGGTACAACTGATTATGCTGTCGCTATTTTCTATGATGCATTGAAAAAAGGTCATTATA
ATTGCTATCTTAAACCTGACACAATGTTGCCAATGATTCACATTAAAGATTGTTTGAGAT
CTTTAATGGAATTTATGGCTGTCCCTGAAGAGAAATTAAAACGAAGAGTTTATAATGTGA
CAGCAATGTCATTTACTCCTGAAGAGTTAGCTGAAAAAATTTCAAAGCATGTGCCACAAC
TTTCAATATCTTATAATCCAGATAGTCGTCAAGCAATTGCTGATGATTGGCCACAGGTTT
TTGATGATTCAGAAGCAAGAGCTGATTGGGGATGGAAGCATGAATATGATTTAGATAGAC
TTGTTGTTGATATTCTTAAAGAAGTTCGTGGACGTTTAAATATGAAACATTAA
>g421.t2 Gene=g421 Length=219
MHNMVELAKQYKLRIFVPSTIGAFGPDSPRNPTPNLTIQRPRTIYGVSKVHAELMGEYYH
HKFGTDFRCLRFPGVISADPPGGGTTDYAVAIFYDALKKGHYNCYLKPDTMLPMIHIKDC
LRSLMEFMAVPEEKLKRRVYNVTAMSFTPEELAEKISKHVPQLSISYNPDSRQAIADDWP
QVFDDSEARADWGWKHEYDLDRLVVDILKEVRGRLNMKH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g421.t2 | Gene3D | G3DSA:3.40.50.720 | - | 1 | 218 | 0 |
| 2 | g421.t2 | PANTHER | PTHR42687 | L-THREONINE 3-DEHYDROGENASE | 1 | 215 | 0 |
| 1 | g421.t2 | Pfam | PF01370 | NAD dependent epimerase/dehydratase family | 3 | 142 | 0 |
| 3 | g421.t2 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 2 | 214 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
