General information for g429.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Isocitrate dehydrogenase [NADP], mitochondrial.
Parent gene
- The parent gene g429 is located on chr_3: 3321162..3327884 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g429 | g429.t5 | TTS | g429.t5 | 3321026 | 3321026 |
| chr_3 | g429 | g429.t5 | isoform | g429.t5 | 3321162 | 3324039 |
| chr_3 | g429 | g429.t5 | exon | g429.t5.exon1 | 3321162 | 3322301 |
| chr_3 | g429 | g429.t5 | cds | g429.t5.CDS1 | 3321162 | 3322301 |
| chr_3 | g429 | g429.t5 | exon | g429.t5.exon2 | 3322446 | 3322513 |
| chr_3 | g429 | g429.t5 | cds | g429.t5.CDS2 | 3322446 | 3322513 |
| chr_3 | g429 | g429.t5 | exon | g429.t5.exon3 | 3323952 | 3324039 |
| chr_3 | g429 | g429.t5 | cds | g429.t5.CDS3 | 3323952 | 3324039 |
| chr_3 | g429 | g429.t5 | TSS | g429.t5 | 3324139 | 3324139 |
Sequences
>g429.t5 Gene=g429 Length=1296
ATGGCTTTTGCATTGAGTAAAATTGCCAAGTGCATCAATAAAAATGCACTTGTCACTCCT
TTATCATCAGCAATGTCATCAAGAAACTACTCTAGTGGAAGCCGAGTTGAAGTAGCAAAA
CCAGTTGTTGAAATGGACGGAGATGAAATGACTCGCATTTTCCCATATGTCAAAGTTGAA
TGTCTCTATTATGATCTTGGTTTGCCATATCGTGATCAGACAGACGATCAAGTTACAATT
GATGCAGCTCATGCAATCTTGAAGCACAATGTAGGCATTAAGTGTGCAACTATCACTCCC
GATGAACAACGTGTTGAGGAATTCAAATTGAAAAAAATGTGGTTGTCACCAAATGGTACC
ATAAGAAACATTTTGGGTGGCACTGTGTTCCGTGAGCCAATTTTGTGCAAAAATATTCCA
CGTCTTGTCCCAGGCTGGACAAATCCTATCGTAATTGGACGTCATGCTTTCGGTGATCAA
TACAGAGCTAAAGATTTAGTAATTTCAAAACCTGGCACAGTTTCACTCAAATTTGTCGGC
GATGACGGTAAAGTTGAAGAGACTGAAGTTTTCCACTTCAAAAATGGAGGTGTAATGCTT
GGCATGTATAACACTGATGATTCAATTGCTGCATTCGCTCATTCATCATTCCAAGTCGCT
TTGCAAAAGGGATGGCCACTTTATATGAGTACCAAAAATACTATTCTTAAGAAATACGAT
GGTCGCTTCAAAGATATCTTCCAAGAAATTTACGAAAAGCACTACGAAGCTAAATTTAAT
GAAGCTAAAATTTGGTATGAACATCGTTTGATTGATGACATGGTTGCACAAGCTTTAAAA
TCATCAGGCAAATTTGTATGGGCTTGTAAAAACTACGATGGTGATGTACAGTCAGATATT
GTTGCCCAAGGTTACGGTAGTTTAGGTTTAATGACAAGTGTTTTGATGTGTCCTGATGGT
AAAACAATTGAAGCTGAAGCTGCTCACGGAACTGTCACACGTCATTTTCGACAACATCAA
CAAGGAAAACCAACAAGCACTAATCCTATTGCTTCAATCTTTGCATGGACTCGAGGTTTC
GAGCATCGTGCTAAACTCGATAACAATCCAGTTCTCGCTAGATTCGCTCAATCACTCGAA
AAAGCATGCATTGACACAGTTGAGTCTGGAAAAATGACAAAAGATTTGGCTGGTTGTATT
CATGGTTTAAGTAACGTTAAAGAAGGCATGTACTTGAACACAGAAGATTTCTTACAAGCT
ATTGCTGAAGAATTGAATCGCTCATGGAAAAAATAA
>g429.t5 Gene=g429 Length=431
MAFALSKIAKCINKNALVTPLSSAMSSRNYSSGSRVEVAKPVVEMDGDEMTRIFPYVKVE
CLYYDLGLPYRDQTDDQVTIDAAHAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGT
IRNILGGTVFREPILCKNIPRLVPGWTNPIVIGRHAFGDQYRAKDLVISKPGTVSLKFVG
DDGKVEETEVFHFKNGGVMLGMYNTDDSIAAFAHSSFQVALQKGWPLYMSTKNTILKKYD
GRFKDIFQEIYEKHYEAKFNEAKIWYEHRLIDDMVAQALKSSGKFVWACKNYDGDVQSDI
VAQGYGSLGLMTSVLMCPDGKTIEAEAAHGTVTRHFRQHQQGKPTSTNPIASIFAWTRGF
EHRAKLDNNPVLARFAQSLEKACIDTVESGKMTKDLAGCIHGLSNVKEGMYLNTEDFLQA
IAEELNRSWKK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g429.t5 | Gene3D | G3DSA:3.40.718.10 | Isopropylmalate Dehydrogenase | 33 | 431 | 1.6E-216 |
| 2 | g429.t5 | PANTHER | PTHR11822:SF21 | ISOCITRATE DEHYDROGENASE [NADP], MITOCHONDRIAL | 34 | 429 | 1.1E-209 |
| 3 | g429.t5 | PANTHER | PTHR11822 | NADP-SPECIFIC ISOCITRATE DEHYDROGENASE | 34 | 429 | 1.1E-209 |
| 6 | g429.t5 | PIRSF | PIRSF000108 | IDH_NADP | 15 | 431 | 1.6E-217 |
| 1 | g429.t5 | Pfam | PF00180 | Isocitrate/isopropylmalate dehydrogenase | 56 | 420 | 6.0E-74 |
| 7 | g429.t5 | ProSitePatterns | PS00470 | Isocitrate and isopropylmalate dehydrogenases signature. | 291 | 310 | - |
| 8 | g429.t5 | SMART | SM01329 | Iso_dh_2 | 41 | 421 | 3.9E-124 |
| 4 | g429.t5 | SUPERFAMILY | SSF53659 | Isocitrate/Isopropylmalate dehydrogenase-like | 35 | 428 | 3.06E-148 |
| 9 | g429.t5 | TIGRFAM | TIGR00127 | nadp_idh_euk: isocitrate dehydrogenase, NADP-dependent | 35 | 428 | 1.3E-206 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004450 | isocitrate dehydrogenase (NADP+) activity | MF |
| GO:0000287 | magnesium ion binding | MF |
| GO:0016616 | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | MF |
| GO:0006102 | isocitrate metabolic process | BP |
| GO:0051287 | NAD binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K00031 (isocitrate dehydrogenase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed