General information for g4310.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Parent gene
- The parent gene g4310 is located on chr_2: 1123022..1126022 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4310 | g4310.t1 | isoform | g4310.t1 | 1123022 | 1125151 |
| chr_2 | g4310 | g4310.t1 | exon | g4310.t1.exon1 | 1123022 | 1123134 |
| chr_2 | g4310 | g4310.t1 | cds | g4310.t1.CDS1 | 1123022 | 1123134 |
| chr_2 | g4310 | g4310.t1 | exon | g4310.t1.exon2 | 1123327 | 1123419 |
| chr_2 | g4310 | g4310.t1 | cds | g4310.t1.CDS2 | 1123327 | 1123419 |
| chr_2 | g4310 | g4310.t1 | exon | g4310.t1.exon3 | 1123477 | 1123804 |
| chr_2 | g4310 | g4310.t1 | cds | g4310.t1.CDS3 | 1123477 | 1123804 |
| chr_2 | g4310 | g4310.t1 | exon | g4310.t1.exon4 | 1123865 | 1124005 |
| chr_2 | g4310 | g4310.t1 | cds | g4310.t1.CDS4 | 1123865 | 1124005 |
| chr_2 | g4310 | g4310.t1 | exon | g4310.t1.exon5 | 1124078 | 1124461 |
| chr_2 | g4310 | g4310.t1 | cds | g4310.t1.CDS5 | 1124078 | 1124461 |
| chr_2 | g4310 | g4310.t1 | exon | g4310.t1.exon6 | 1124528 | 1124607 |
| chr_2 | g4310 | g4310.t1 | cds | g4310.t1.CDS6 | 1124528 | 1124607 |
| chr_2 | g4310 | g4310.t1 | exon | g4310.t1.exon7 | 1124671 | 1124844 |
| chr_2 | g4310 | g4310.t1 | cds | g4310.t1.CDS7 | 1124671 | 1124844 |
| chr_2 | g4310 | g4310.t1 | exon | g4310.t1.exon8 | 1125064 | 1125151 |
| chr_2 | g4310 | g4310.t1 | cds | g4310.t1.CDS8 | 1125064 | 1125151 |
| chr_2 | g4310 | g4310.t1 | TSS | g4310.t1 | 1126023 | 1126023 |
| chr_2 | g4310 | g4310.t1 | TTS | g4310.t1 | NA | NA |
Sequences
>g4310.t1 Gene=g4310 Length=1401
ATGTTGCGACAACTCATGAGTTATGTTAAAAACTGCAGTAGAAAAAGAAAATATGTTGAA
GATGATGATGAAAAGGGAAAACGAGAAGATCTCTCGAATGGAAATATCTTGAACGGCAAT
ATGGCAGCAAATAATTTTGTCAAACCTTGCCAACTGAAAGCACCTTTAGTTGTGACAATT
GTAGGATTACCCTTCCGCGGCAAATCACTCGCTGCTCATAAAATCTCGAGAAATCTCTTT
TGGAAGGGTGAGAAAGTGAAAGTATTCGAAGTTGAAGAGAGCGCTAATGAGGAAACCCTA
AAAAGCATAAGCGAGTGGTTTGGAAACTCAAATAATGTTGCCATAATCGACGGAATGCAT
TTAACAAAACAATCACGCACCCTTGTTGGCAACTATTGTCGTAAAAATGTCTACCATCAT
CTTATCGTTGAATTTGAATGTGAACCGGACTGTGTTGAAGAAAATATCAACGATACTGCT
GAATTTTATCAAAAGATTGACAACAATCAAGATTGGCTCCAAACGTTCCGTGAGAAGAAT
GCCAAATACAAATCAAGTTTTGAAAAGTGCTTACCACAAGAAGGAGCACTTATTAATGTT
ACAAACTCTGAAAATGTATTAGTTCATTCAGTTAGAGCGAGAGGTGTTCAAGGATTATTA
CAGACAATCATATTGGGTGTGCTATCAAGTCCAGTCATAAAACATCGTGTATTCTACTTT
AGTAGACATGGTGAATCAGAATTCAATGTGCTTGGTCGTATTGGTGGTGACGCAGATTTA
TCACCAAGAGGTAAAAAATATAGTGAAAATCTTGCTAAACAATTAGGTGGTCCAGGAAGT
AGCCCTAATGCACCAAAGCCAAGATTAATATGGACTTCAGAGCTCCAACGTACAATTCAT
ACTGTTCAAAATATTCCCGGACCACGAACAGCTCTCAAAGATCTTAATGAAATAAATGCA
GGTATTTGTGAGGGTTTGACATATGAGGAAATTCAAGAGCGTTTTCCACAAGAATTTGCA
TGGCGTGACGCAGATAAATTGAAATATCGTTATCCCCATGGCGAGAGTTATTTGGATTTA
TTGCAAAGAGTTGATGGTGTCGTTCAAGCACTTTTATTGAACACTGAAGTTTTGGTCGTA
TCACATCAAGCAGTTCTTAGATGCATTTTAGCTTATTTCAAAGGAAGCAAACCAGAAGAA
ATTCCGTACGTCAATGTGCCACTTCATACGCTTTTAATCGTAAAATCACATGGCTATGAT
TTCAAAATTGAAACTGTCCCCTTAAAAGTGGAGTGCGTTGACACATATCGCATGCAGCCA
TCGAATTGCTCTATAATGCGTACTACACAAGAAGCGCTTACCACAGTACCACAACATTTC
GACGCACCGCTTATTAATTAA
>g4310.t1 Gene=g4310 Length=466
MLRQLMSYVKNCSRKRKYVEDDDEKGKREDLSNGNILNGNMAANNFVKPCQLKAPLVVTI
VGLPFRGKSLAAHKISRNLFWKGEKVKVFEVEESANEETLKSISEWFGNSNNVAIIDGMH
LTKQSRTLVGNYCRKNVYHHLIVEFECEPDCVEENINDTAEFYQKIDNNQDWLQTFREKN
AKYKSSFEKCLPQEGALINVTNSENVLVHSVRARGVQGLLQTIILGVLSSPVIKHRVFYF
SRHGESEFNVLGRIGGDADLSPRGKKYSENLAKQLGGPGSSPNAPKPRLIWTSELQRTIH
TVQNIPGPRTALKDLNEINAGICEGLTYEEIQERFPQEFAWRDADKLKYRYPHGESYLDL
LQRVDGVVQALLLNTEVLVVSHQAVLRCILAYFKGSKPEEIPYVNVPLHTLLIVKSHGYD
FKIETVPLKVECVDTYRMQPSNCSIMRTTQEALTTVPQHFDAPLIN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g4310.t1 | CDD | cd07067 | HP_PGM_like | 239 | 428 | 0e+00 |
| 13 | g4310.t1 | Gene3D | G3DSA:3.40.50.300 | - | 54 | 94 | 3e-07 |
| 12 | g4310.t1 | Gene3D | G3DSA:3.40.50.300 | - | 95 | 230 | 0e+00 |
| 14 | g4310.t1 | Gene3D | G3DSA:3.40.50.1240 | - | 236 | 459 | 0e+00 |
| 3 | g4310.t1 | PANTHER | PTHR10606 | 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE | 33 | 93 | 0e+00 |
| 5 | g4310.t1 | PANTHER | PTHR10606:SF65 | 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2, 6-BISPHOSPHATASE-LIKE PROTEIN | 33 | 93 | 0e+00 |
| 4 | g4310.t1 | PANTHER | PTHR10606 | 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE | 92 | 464 | 0e+00 |
| 6 | g4310.t1 | PANTHER | PTHR10606:SF65 | 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2, 6-BISPHOSPHATASE-LIKE PROTEIN | 92 | 464 | 0e+00 |
| 16 | g4310.t1 | PIRSF | PIRSF000709 | 6PFK_fruc_bisph_Ptase | 3 | 98 | 0e+00 |
| 15 | g4310.t1 | PIRSF | PIRSF000709 | 6PFK_fruc_bisph_Ptase | 91 | 455 | 0e+00 |
| 7 | g4310.t1 | PRINTS | PR00991 | 6-phosphofructo-2-kinase family signature | 113 | 127 | 0e+00 |
| 9 | g4310.t1 | PRINTS | PR00991 | 6-phosphofructo-2-kinase family signature | 238 | 260 | 0e+00 |
| 8 | g4310.t1 | PRINTS | PR00991 | 6-phosphofructo-2-kinase family signature | 320 | 336 | 0e+00 |
| 1 | g4310.t1 | Pfam | PF01591 | 6-phosphofructo-2-kinase | 92 | 220 | 2e-07 |
| 2 | g4310.t1 | Pfam | PF00300 | Histidine phosphatase superfamily (branch 1) | 238 | 425 | 0e+00 |
| 18 | g4310.t1 | SMART | SM00855 | PGAM_5 | 237 | 389 | 0e+00 |
| 11 | g4310.t1 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 55 | 188 | 0e+00 |
| 10 | g4310.t1 | SUPERFAMILY | SSF53254 | Phosphoglycerate mutase-like | 238 | 454 | 0e+00 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006003 | fructose 2,6-bisphosphate metabolic process | BP |
| GO:0005524 | ATP binding | MF |
| GO:0006000 | fructose metabolic process | BP |
| GO:0003824 | catalytic activity | MF |
| GO:0003873 | 6-phosphofructo-2-kinase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
