General information for g4310.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Parent gene
- The parent gene g4310 is located on chr_2: 1123022..1126022 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4310 | g4310.t3 | isoform | g4310.t3 | 1121062 | 1124461 |
| chr_2 | g4310 | g4310.t3 | exon | g4310.t3.exon1 | 1121062 | 1122503 |
| chr_2 | g4310 | g4310.t3 | cds | g4310.t3.CDS1 | 1122469 | 1122503 |
| chr_2 | g4310 | g4310.t3 | exon | g4310.t3.exon2 | 1123327 | 1123419 |
| chr_2 | g4310 | g4310.t3 | cds | g4310.t3.CDS2 | 1123327 | 1123419 |
| chr_2 | g4310 | g4310.t3 | exon | g4310.t3.exon3 | 1123477 | 1123804 |
| chr_2 | g4310 | g4310.t3 | cds | g4310.t3.CDS3 | 1123477 | 1123804 |
| chr_2 | g4310 | g4310.t3 | exon | g4310.t3.exon4 | 1123865 | 1124005 |
| chr_2 | g4310 | g4310.t3 | cds | g4310.t3.CDS4 | 1123865 | 1124005 |
| chr_2 | g4310 | g4310.t3 | exon | g4310.t3.exon5 | 1124078 | 1124461 |
| chr_2 | g4310 | g4310.t3 | cds | g4310.t3.CDS5 | 1124078 | 1124449 |
| chr_2 | g4310 | g4310.t3 | TSS | g4310.t3 | NA | NA |
| chr_2 | g4310 | g4310.t3 | TTS | g4310.t3 | NA | NA |
Sequences
>g4310.t3 Gene=g4310 Length=2388
ATAATCGACGGAATGCATTTAACAAAACAATCACGCACCCTTGTTGGCAACTATTGTCGT
AAAAATGTCTACCATCATCTTATCGTTGAATTTGAATGTGAACCGGACTGTGTTGAAGAA
AATATCAACGATACTGCTGAATTTTATCAAAAGATTGACAACAATCAAGATTGGCTCCAA
ACGTTCCGTGAGAAGAATGCCAAATACAAATCAAGTTTTGAAAAGTGCTTACCACAAGAA
GGAGCACTTATTAATGTTACAAACTCTGAAAATGTATTAGTTCATTCAGTTAGAGCGAGA
GGTGTTCAAGGATTATTACAGACAATCATATTGGGTGTGCTATCAAGTCCAGTCATAAAA
CATCGTGTATTCTACTTTAGTAGACATGGTGAATCAGAATTCAATGTGCTTGGTCGTATT
GGTGGTGACGCAGATTTATCACCAAGAGGTAAAAAATATAGTGAAAATCTTGCTAAACAA
TTAGGTGGTCCAGGAAGTAGCCCTAATGCACCAAAGCCAAGATTAATATGGACTTCAGAG
CTCCAACGTACAATTCATACTGTTCAAAATATTCCCGGACCACGAACAGCTCTCAAAGAT
CTTAATGAAATAAATGCAGGTATTTGTGAGGGTTTGACATATGAGGAAATTCAAGAGCGT
TTTCCACAAGAATTTGCATGGCGTGACGCAGATAAATTGAAATATCGTTATCCCCATGGC
GAGAGTTATTTGGATTTATTGCAAAGAGTTGATGGTGTCGTTCAAGCACTTTTATTGAAC
ACTGAAGTTTTGGTCGTATCACATCAAGCAGTTCTTAGATGCATTTTAGCTTATTTCAAA
GGAAGCAAACCAGAAGAAATTCCGTACGTCAATGTGCCACTTCATACGCTTTTAATCGTA
AAATCACATGGCTATGATTTCAAAATTGAAACTGTCCCCTTAAAAGACATAGCGTTTATT
GACAAGACTTTATTTCTATAGATAGACAAAGAACAAGTCAACAAAATTAATTTGAATTCA
TATTTTAAAATAAAATTTTCTCCCAATGAGAAATGCAAGAAATTTTTACACAAAACATTT
AAGTTTAAGGAAAATTTTTACGCATGAAAAATTGGCAAATAGAATGTAATAATATTTCAA
TCAAAAATAAGTTTTAAGAAAACAACTTTAATTTGACCGAAGTCTCTTTTAGATTTTTCC
TCTAAATGACATTCAAGTTTCGATAGCACGTTTTTTTTCTTCAAGGTCATTTCTCGTCGA
GAATATCATGAGAAATGAGAGAAAGAGTGGGGAAATGAAAGAAAATTTCACTTTCTTTCT
TTTTTTATTTGCCATATGCTCTGATGCTTTTTACATCTCATATTTAAGATAGAAGACAAA
AAAATTATTTATAAATAATGAAGTGCACAACATATTTTTTGCTTAAAATTTATCACAATT
TAAGAGAGTAAAATAAAATTGATAATAAATTGGAACTTTAGATTACCTGATCCAAACACA
CAAAAATGAATTAAATAATAGAAAAAAGAGTATGATATTTAAATTATAAGTACATACATA
CACAAGTTAAACTTCCAAAAAACGAAAAATAATTGATGTCTATGCAAAAATTCTATTTAA
GAAAATGTTACTACTCAAGTAGTTAATTGATTAAAAAGAATATTTGAATAAAAACCTTTA
ATCGAGTCAATTAACTTTTATGCACACTAATAGCTTCTTGTGATGCACAAAAAATGAGAA
TTTTTTGCGTAGGCAGCTTTTGAAATAAGTTTTTTATTTTCATTAATGAAAAGTTACGCA
ATGCAAATATGTCTACATCGATGAAAAATAAAACCATAAAATTTTAATGAATCAGGATGA
TAGAAACAGACACAAATAAACTACATAACCACTAACTGCCAATCGTTATCGTCAAAAAAC
AGATTTAAAATTACACAGAGAGAAAACAAAAGAAAAAAATATTAAACACAATCACATTTC
TAAGAGACAAAAAAACTTGCAATTTTAAAATGGTTAGAAAAAAATGTTTTAAATTATTTC
AGGAGAACTCCAAAAAATGTCATTATCAATTAGAGTCATAATTAACATCCTTTAACATAG
AAATAGAAATACAGATATAAATAAAACAATTTATCTCTTTTTATACAAAGAAAGCTTATT
TCTAATTATGTAAAGATGAGGAAGATATTTGAAATGAATTTCATAATGAACGAAGAAGAA
GGAGATGGTGAAAATGGATAAATGAATTTAATTTTAGTTTTTTTACATTGCTATTGTGTT
AGTTTTTTGTGATAATTCAATAACATGAAAAAATTTCAAATAAAAAATTTACCTACATAA
TATTTGAAAGAGACAAACATAATTGAAATAAAATTATATCCATATATT
>g4310.t3 Gene=g4310 Length=322
MHLTKQSRTLVGNYCRKNVYHHLIVEFECEPDCVEENINDTAEFYQKIDNNQDWLQTFRE
KNAKYKSSFEKCLPQEGALINVTNSENVLVHSVRARGVQGLLQTIILGVLSSPVIKHRVF
YFSRHGESEFNVLGRIGGDADLSPRGKKYSENLAKQLGGPGSSPNAPKPRLIWTSELQRT
IHTVQNIPGPRTALKDLNEINAGICEGLTYEEIQERFPQEFAWRDADKLKYRYPHGESYL
DLLQRVDGVVQALLLNTEVLVVSHQAVLRCILAYFKGSKPEEIPYVNVPLHTLLIVKSHG
YDFKIETVPLKDIAFIDKTLFL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g4310.t3 | CDD | cd07067 | HP_PGM_like | 121 | 310 | 0e+00 |
| 7 | g4310.t3 | Gene3D | G3DSA:3.40.50.300 | - | 1 | 112 | 2e-07 |
| 8 | g4310.t3 | Gene3D | G3DSA:3.40.50.1240 | - | 118 | 318 | 0e+00 |
| 2 | g4310.t3 | PANTHER | PTHR10606 | 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE | 1 | 311 | 0e+00 |
| 3 | g4310.t3 | PANTHER | PTHR10606:SF65 | 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2, 6-BISPHOSPHATASE-LIKE PROTEIN | 1 | 311 | 0e+00 |
| 9 | g4310.t3 | PIRSF | PIRSF000709 | 6PFK_fruc_bisph_Ptase | 1 | 319 | 0e+00 |
| 5 | g4310.t3 | PRINTS | PR00991 | 6-phosphofructo-2-kinase family signature | 120 | 142 | 0e+00 |
| 4 | g4310.t3 | PRINTS | PR00991 | 6-phosphofructo-2-kinase family signature | 202 | 218 | 0e+00 |
| 1 | g4310.t3 | Pfam | PF00300 | Histidine phosphatase superfamily (branch 1) | 120 | 307 | 0e+00 |
| 11 | g4310.t3 | SMART | SM00855 | PGAM_5 | 119 | 271 | 0e+00 |
| 6 | g4310.t3 | SUPERFAMILY | SSF53254 | Phosphoglycerate mutase-like | 120 | 311 | 0e+00 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006003 | fructose 2,6-bisphosphate metabolic process | BP |
| GO:0005524 | ATP binding | MF |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
