General information for g4310.t6
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Parent gene
- The parent gene g4310 is located on chr_2: 1123022..1126022 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4310 | g4310.t6 | isoform | g4310.t6 | 1122503 | 1126022 |
| chr_2 | g4310 | g4310.t6 | exon | g4310.t6.exon1 | 1122503 | 1123134 |
| chr_2 | g4310 | g4310.t6 | cds | g4310.t6.CDS1 | 1123022 | 1123134 |
| chr_2 | g4310 | g4310.t6 | exon | g4310.t6.exon2 | 1123327 | 1123419 |
| chr_2 | g4310 | g4310.t6 | cds | g4310.t6.CDS2 | 1123327 | 1123419 |
| chr_2 | g4310 | g4310.t6 | exon | g4310.t6.exon3 | 1123477 | 1123804 |
| chr_2 | g4310 | g4310.t6 | cds | g4310.t6.CDS3 | 1123477 | 1123804 |
| chr_2 | g4310 | g4310.t6 | exon | g4310.t6.exon4 | 1123865 | 1124005 |
| chr_2 | g4310 | g4310.t6 | cds | g4310.t6.CDS4 | 1123865 | 1124005 |
| chr_2 | g4310 | g4310.t6 | exon | g4310.t6.exon5 | 1124078 | 1124461 |
| chr_2 | g4310 | g4310.t6 | cds | g4310.t6.CDS5 | 1124078 | 1124461 |
| chr_2 | g4310 | g4310.t6 | exon | g4310.t6.exon6 | 1124528 | 1124607 |
| chr_2 | g4310 | g4310.t6 | cds | g4310.t6.CDS6 | 1124528 | 1124607 |
| chr_2 | g4310 | g4310.t6 | exon | g4310.t6.exon7 | 1124671 | 1124844 |
| chr_2 | g4310 | g4310.t6 | cds | g4310.t6.CDS7 | 1124671 | 1124812 |
| chr_2 | g4310 | g4310.t6 | exon | g4310.t6.exon8 | 1125803 | 1126022 |
| chr_2 | g4310 | g4310.t6 | TSS | g4310.t6 | 1126023 | 1126023 |
| chr_2 | g4310 | g4310.t6 | TTS | g4310.t6 | NA | NA |
Sequences
>g4310.t6 Gene=g4310 Length=2052
GTTTTCTCGAGTCGATGTTGGAATGAAGACAGAAAATTTGCATAGCTCTCAAAATTGCCT
GAGATCCATTAATTATATCAAATTTTCACCACCAGACTGCTTTGTGTTTGCTTAAAACCA
ATGATTAGTGAATTACTGAAGTATTGTGCCTAAAAATAAGTTATATTGGCGTGTTTGTTC
GGGTCAGAACGAAGCAGAAATGAAATTTTTTGATATTTCGATCTCTCGAATGGAAATATC
TTGAACGGCAATATGGCAGCAAATAATTTTGTCAAACCTTGCCAACTGAAAGCACCTTTA
GTTGTGACAATTGTAGGATTACCCTTCCGCGGCAAATCACTCGCTGCTCATAAAATCTCG
AGAAATCTCTTTTGGAAGGGTGAGAAAGTGAAAGTATTCGAAGTTGAAGAGAGCGCTAAT
GAGGAAACCCTAAAAAGCATAAGCGAGTGGTTTGGAAACTCAAATAATGTTGCCATAATC
GACGGAATGCATTTAACAAAACAATCACGCACCCTTGTTGGCAACTATTGTCGTAAAAAT
GTCTACCATCATCTTATCGTTGAATTTGAATGTGAACCGGACTGTGTTGAAGAAAATATC
AACGATACTGCTGAATTTTATCAAAAGATTGACAACAATCAAGATTGGCTCCAAACGTTC
CGTGAGAAGAATGCCAAATACAAATCAAGTTTTGAAAAGTGCTTACCACAAGAAGGAGCA
CTTATTAATGTTACAAACTCTGAAAATGTATTAGTTCATTCAGTTAGAGCGAGAGGTGTT
CAAGGATTATTACAGACAATCATATTGGGTGTGCTATCAAGTCCAGTCATAAAACATCGT
GTATTCTACTTTAGTAGACATGGTGAATCAGAATTCAATGTGCTTGGTCGTATTGGTGGT
GACGCAGATTTATCACCAAGAGGTAAAAAATATAGTGAAAATCTTGCTAAACAATTAGGT
GGTCCAGGAAGTAGCCCTAATGCACCAAAGCCAAGATTAATATGGACTTCAGAGCTCCAA
CGTACAATTCATACTGTTCAAAATATTCCCGGACCACGAACAGCTCTCAAAGATCTTAAT
GAAATAAATGCAGGTATTTGTGAGGGTTTGACATATGAGGAAATTCAAGAGCGTTTTCCA
CAAGAATTTGCATGGCGTGACGCAGATAAATTGAAATATCGTTATCCCCATGGCGAGAGT
TATTTGGATTTATTGCAAAGAGTTGATGGTGTCGTTCAAGCACTTTTATTGAACACTGAA
GTTTTGGTCGTATCACATCAAGCAGTTCTTAGATGCATTTTAGCTTATTTCAAAGGAAGC
AAACCAGAAGAAATTCCGTACGTCAATGTGCCACTTCATACGCTTTTAATCGTAAAATCA
CATGGCTATGATTTCAAAATTGAAACTGTCCCCTTAAAAGTGGAGTGCGTTGACACATAT
CGCATGCAGCCATCGAATTGCTCTATAATGCGTACTACACAAGAAGCGCTTACCACAGTA
CCACAACATTTCGACGCACCGCTTATTAATTAACATAAGGAGGACTATGAATCTTCAAGA
GCGCCAGCTAAGCAAATTAAATTAAGCAATTGAACTGCAAACTCATTACACATGATTCAC
TATTTTATTTTTTCGCTTCAATGCAAATGAGAGAAAGACTTTACAGACAGTCTAAAAAAT
ATTGAAAAACAAAAATAACTTTCATTATCATTCTTCTATTATTAATCGTTCTTCTGTCTT
CGTAGTTTTAATTGATTTGACAAGCAAAAAACCTTTTTATTGTGTTTTTAAATAGTACTT
TTTGTTTTTTCCTTATCATAATTCAAAATTGCATTTTTTTGATTTTTTCTTTTCTGCCAA
TTTAAATAATCAATTTTTTTTTCAAACTACATATCGCTCTATATTGTAGCACATACTAAT
TTTAGTTTTTCTTTTCTTTTTTAAAACTGTAGTAATTTTGATTTTTTTAATAATTAAAAT
TTAATTTAATTTGATTGTGAGTTGAATTTTTAGTTATATAATCAATGTATTTCTATATTT
TTTATTCATAGA
>g4310.t6 Gene=g4310 Length=426
MAANNFVKPCQLKAPLVVTIVGLPFRGKSLAAHKISRNLFWKGEKVKVFEVEESANEETL
KSISEWFGNSNNVAIIDGMHLTKQSRTLVGNYCRKNVYHHLIVEFECEPDCVEENINDTA
EFYQKIDNNQDWLQTFREKNAKYKSSFEKCLPQEGALINVTNSENVLVHSVRARGVQGLL
QTIILGVLSSPVIKHRVFYFSRHGESEFNVLGRIGGDADLSPRGKKYSENLAKQLGGPGS
SPNAPKPRLIWTSELQRTIHTVQNIPGPRTALKDLNEINAGICEGLTYEEIQERFPQEFA
WRDADKLKYRYPHGESYLDLLQRVDGVVQALLLNTEVLVVSHQAVLRCILAYFKGSKPEE
IPYVNVPLHTLLIVKSHGYDFKIETVPLKVECVDTYRMQPSNCSIMRTTQEALTTVPQHF
DAPLIN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g4310.t6 | CDD | cd07067 | HP_PGM_like | 199 | 388 | 0e+00 |
| 11 | g4310.t6 | Gene3D | G3DSA:3.40.50.300 | - | 14 | 54 | 3e-07 |
| 10 | g4310.t6 | Gene3D | G3DSA:3.40.50.300 | - | 55 | 190 | 0e+00 |
| 12 | g4310.t6 | Gene3D | G3DSA:3.40.50.1240 | - | 196 | 419 | 0e+00 |
| 3 | g4310.t6 | PANTHER | PTHR10606 | 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE | 5 | 424 | 0e+00 |
| 4 | g4310.t6 | PANTHER | PTHR10606:SF65 | 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2, 6-BISPHOSPHATASE-LIKE PROTEIN | 5 | 424 | 0e+00 |
| 14 | g4310.t6 | PIRSF | PIRSF000709 | 6PFK_fruc_bisph_Ptase | 3 | 58 | 0e+00 |
| 13 | g4310.t6 | PIRSF | PIRSF000709 | 6PFK_fruc_bisph_Ptase | 52 | 415 | 0e+00 |
| 7 | g4310.t6 | PRINTS | PR00991 | 6-phosphofructo-2-kinase family signature | 73 | 87 | 0e+00 |
| 6 | g4310.t6 | PRINTS | PR00991 | 6-phosphofructo-2-kinase family signature | 198 | 220 | 0e+00 |
| 5 | g4310.t6 | PRINTS | PR00991 | 6-phosphofructo-2-kinase family signature | 280 | 296 | 0e+00 |
| 1 | g4310.t6 | Pfam | PF01591 | 6-phosphofructo-2-kinase | 52 | 180 | 1e-07 |
| 2 | g4310.t6 | Pfam | PF00300 | Histidine phosphatase superfamily (branch 1) | 198 | 385 | 0e+00 |
| 16 | g4310.t6 | SMART | SM00855 | PGAM_5 | 197 | 349 | 0e+00 |
| 9 | g4310.t6 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 15 | 148 | 0e+00 |
| 8 | g4310.t6 | SUPERFAMILY | SSF53254 | Phosphoglycerate mutase-like | 198 | 414 | 0e+00 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006003 | fructose 2,6-bisphosphate metabolic process | BP |
| GO:0005524 | ATP binding | MF |
| GO:0006000 | fructose metabolic process | BP |
| GO:0003824 | catalytic activity | MF |
| GO:0003873 | 6-phosphofructo-2-kinase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
