General information for g4399.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Phospholipase A2 group XV.
Parent gene
- The parent gene g4399 is located on chr_2: 1971798..1973006 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4399 | g4399.t5 | TSS | g4399.t5 | 1970119 | 1970119 |
| chr_2 | g4399 | g4399.t5 | isoform | g4399.t5 | 1970234 | 1971138 |
| chr_2 | g4399 | g4399.t5 | exon | g4399.t5.exon1 | 1970234 | 1970378 |
| chr_2 | g4399 | g4399.t5 | cds | g4399.t5.CDS1 | 1970234 | 1970378 |
| chr_2 | g4399 | g4399.t5 | exon | g4399.t5.exon2 | 1970436 | 1970679 |
| chr_2 | g4399 | g4399.t5 | cds | g4399.t5.CDS2 | 1970436 | 1970679 |
| chr_2 | g4399 | g4399.t5 | exon | g4399.t5.exon3 | 1970738 | 1970868 |
| chr_2 | g4399 | g4399.t5 | cds | g4399.t5.CDS3 | 1970738 | 1970868 |
| chr_2 | g4399 | g4399.t5 | exon | g4399.t5.exon4 | 1970943 | 1971138 |
| chr_2 | g4399 | g4399.t5 | cds | g4399.t5.CDS4 | 1970943 | 1971136 |
| chr_2 | g4399 | g4399.t5 | TTS | g4399.t5 | 1971936 | 1971936 |
Sequences
>g4399.t5 Gene=g4399 Length=716
ATGCGAGAAATTGCAATATTGTTTTTTGGATTTTTAATTTTGCAAATTAATGCACAATTT
TTGCCATGGTTTTTGGAACAAAATTATGTCAATCCGCCTAATAATAACAACAAGCCTTCA
AAGCCAAGATCACCAGTTATTTTCGTTCCGGGAGATGGTGGTACACAAGTGGATGCAAAG
CTCAATAAACCATCAACTAGGCATATTTTCTGTACAAAAACGACAAAAGACTATTTCAAT
GTATGGCTTAATTTAGAACTTATGGCACCATTAATTATTGACTGCTGGACAGATAATGTA
AAACTTTATTACAATAATGAAACTCGAACAACAAGTAACTCGCCAGGTGTTGAATTAAGA
ATCCCAAGATGGGGTGATCCTGAAGTTGTTGAATGGATCGATCCATCAAAAAACCATATT
GGTGCATATTTCAAATCTATTGCAAATGGTCTCGTTCAAAATGGCTACGTCAGAAATGTT
TCAATTCGTGGTGCACCATATGATTTCAGAAAGGGACCATCTGAATTGGGTCAATATTTC
ATTGATTTAAAACAACTCGTAGAAGAAACATATGAGATGAATTCGCAAGTGCCTGTAACT
TTAATTGCACATTCAATGGGCGCACCGATTTTGATGATTTTCTTGCAACAACAAAATGAA
AAATGGAAGGAAAAGTATATCGCTCGAATGATCACAATTGCAGGTGCTTATGGTGG
>g4399.t5 Gene=g4399 Length=238
MREIAILFFGFLILQINAQFLPWFLEQNYVNPPNNNNKPSKPRSPVIFVPGDGGTQVDAK
LNKPSTRHIFCTKTTKDYFNVWLNLELMAPLIIDCWTDNVKLYYNNETRTTSNSPGVELR
IPRWGDPEVVEWIDPSKNHIGAYFKSIANGLVQNGYVRNVSIRGAPYDFRKGPSELGQYF
IDLKQLVEETYEMNSQVPVTLIAHSMGAPILMIFLQQQNEKWKEKYIARMITIAGAYG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g4399.t5 | Gene3D | G3DSA:3.40.50.1820 | - | 90 | 238 | 7.0E-44 |
| 2 | g4399.t5 | PANTHER | PTHR11440:SF47 | GROUP XV PHOSPHOLIPASE A2 | 37 | 235 | 9.0E-69 |
| 3 | g4399.t5 | PANTHER | PTHR11440 | LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED | 37 | 235 | 9.0E-69 |
| 1 | g4399.t5 | Pfam | PF02450 | Lecithin:cholesterol acyltransferase | 78 | 237 | 4.6E-42 |
| 8 | g4399.t5 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 9 | g4399.t5 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 10 | g4399.t5 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 14 | - |
| 11 | g4399.t5 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 18 | - |
| 7 | g4399.t5 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 238 | - |
| 4 | g4399.t5 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 41 | 235 | 4.05E-14 |
| 5 | g4399.t5 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008374 | O-acyltransferase activity | MF |
| GO:0006629 | lipid metabolic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
