Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative cysteine proteinase CG12163.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g4405 g4405.t25 TTS g4405.t25 2001061 2001061
chr_2 g4405 g4405.t25 isoform g4405.t25 2001763 2005157
chr_2 g4405 g4405.t25 exon g4405.t25.exon1 2001763 2002007
chr_2 g4405 g4405.t25 cds g4405.t25.CDS1 2001764 2002007
chr_2 g4405 g4405.t25 exon g4405.t25.exon2 2002061 2003089
chr_2 g4405 g4405.t25 cds g4405.t25.CDS2 2002061 2003089
chr_2 g4405 g4405.t25 exon g4405.t25.exon3 2003160 2003281
chr_2 g4405 g4405.t25 cds g4405.t25.CDS3 2003160 2003281
chr_2 g4405 g4405.t25 exon g4405.t25.exon4 2005077 2005157
chr_2 g4405 g4405.t25 cds g4405.t25.CDS4 2005077 2005157
chr_2 g4405 g4405.t25 TSS g4405.t25 2005223 2005223

Sequences

>g4405.t25 Gene=g4405 Length=1477
ATGTGGAAATTTAAAATTTTGCTAATTTGTTTAGCAAGTATTATAATAGTTAATGCCACA
GAATGTGAAGATTGTCAAAATCGTGCTGCTCGTCAAATCGGTGTTCCCGGTGGAATCAGT
CCTGTAGAAAACTTTGAAGATGTAAAAATTTATGTTCAAGAAGCTATTGATGAAATTAAT
GATAATGAAGATCCTGATTACATTTTGAAACATATCGTTGAAGCAACCCAACAAGTTGTT
GCAGGCATGAGTTATAAAATTAAAGCAGTGTTTTCCAGAGATGGAAGCGACATTGAATGT
GATTTTGATGTATGGGAGCAAGCTTGGATTAAAGATGGACGTAAAGTTTCAGTTTCTTGC
AAAAATGATAAGAAATATAAGTTGACCCAATCACCATCTAATCAGCGTGTCAAACGTGAT
AACACGCTTGAAAGAGTTCTTGGTTTACCATCCAATACTGATGATCATGACGATTTGATA
AAAATACTTTCTGAACATTTGAAGAGACTCGATACTGGAAGTGATGCACAATTTGAATTG
GTAAAACTTGAAAAGGTAACTCAACAAGTAGTAGCTGGAATAAAATATAAAGCAACAGGT
ATTTTTAAAATTGGCAATGAAGAGAAAAAATGTGTTATCGATGTATGGCATCGCTCATGG
ATTAAGGGAGATGAAGGCACTCAATTAAGCGCTGATTGTGATAAAGGTGCAACAACTTTC
AAGACAAAATCTTCTAGAAAAAGGAGATCAGTTCATCACCACACACACAATCGTCACAAT
AGACAATCAGTAAGCGATCATTTTGATGACCATCATCATCATACTGATAGACATCATCAT
CAATACTCAGCTACTGAAGAAATGAAAGAAATAAAATCTGAAATTTTATTTAACAATTTC
ATAACTAAATATAATCGTAAATATGCCAATGAACTTGAACATAAAATGAGAATGAGAATT
TTCAAGAAGAATTTACATAAAATTGAAATGTTGAATAAGCATGAACAAGGCACTGCAAAG
TATGGAATTACAGAATTCGCTGATTTAACTGAAAAGGAATACTTGCATAAAACTGGTTTG
AGAGTGCGTGAAAGACATGAGAATGAATTAGAAAATCCAATTGCACATATTCCAGAAGTT
GAAGATTTACCAACCGAATTTGATTGGAGAGATAAATCAGCAGTTACAAGTGTAAAAAAT
CAAGGAAATTGTGGATCATGCTGGAGTTTTTCTGTTACAGGAAATATTGAAGGCTTACAT
GCTATTAAAACTGGAAAACTTGAAGCTTATTCTGAACAAGAACTTTTGGACTGTGATACA
ACTGATAATGCTTGCAATGGTGGTTATATGGATGATGCTTTTAAAGCAATTGAAAAAATT
GGTGGTCTAGAATTAGAAGATGAATATCCTTATCAAGCAAGGAAACAAAAGAAATGCTTG
TTTAATGCTACTATGAGTCATGTTAAAGTTAAAGGTG

>g4405.t25 Gene=g4405 Length=492
MWKFKILLICLASIIIVNATECEDCQNRAARQIGVPGGISPVENFEDVKIYVQEAIDEIN
DNEDPDYILKHIVEATQQVVAGMSYKIKAVFSRDGSDIECDFDVWEQAWIKDGRKVSVSC
KNDKKYKLTQSPSNQRVKRDNTLERVLGLPSNTDDHDDLIKILSEHLKRLDTGSDAQFEL
VKLEKVTQQVVAGIKYKATGIFKIGNEEKKCVIDVWHRSWIKGDEGTQLSADCDKGATTF
KTKSSRKRRSVHHHTHNRHNRQSVSDHFDDHHHHTDRHHHQYSATEEMKEIKSEILFNNF
ITKYNRKYANELEHKMRMRIFKKNLHKIEMLNKHEQGTAKYGITEFADLTEKEYLHKTGL
RVRERHENELENPIAHIPEVEDLPTEFDWRDKSAVTSVKNQGNCGSCWSFSVTGNIEGLH
AIKTGKLEAYSEQELLDCDTTDNACNGGYMDDAFKAIEKIGGLELEDEYPYQARKQKKCL
FNATMSHVKVKG

Protein features from InterProScan

Transcript Database ID Name Start End E.value
19 g4405.t25 CDD cd00042 CY 37 120 9.91346E-9
18 g4405.t25 CDD cd02248 Peptidase_C1A 384 492 4.79718E-50
11 g4405.t25 Gene3D G3DSA:3.10.450.10 - 31 124 3.6E-16
10 g4405.t25 Gene3D G3DSA:3.10.450.10 - 142 232 6.4E-11
12 g4405.t25 Gene3D G3DSA:3.90.70.10 Cysteine proteinases 245 492 8.6E-63
26 g4405.t25 MobiDBLite mobidb-lite consensus disorder prediction 239 285 -
28 g4405.t25 MobiDBLite mobidb-lite consensus disorder prediction 242 260 -
27 g4405.t25 MobiDBLite mobidb-lite consensus disorder prediction 261 285 -
4 g4405.t25 PANTHER PTHR13814:SF16 CYSTATIN 296 484 1.4E-77
5 g4405.t25 PANTHER PTHR13814 FETUIN 296 484 1.4E-77
2 g4405.t25 Pfam PF00031 Cystatin domain 37 93 2.9E-6
1 g4405.t25 Pfam PF08246 Cathepsin propeptide inhibitor domain (I29) 297 354 2.7E-12
3 g4405.t25 Pfam PF00112 Papain family cysteine protease 383 488 1.8E-36
14 g4405.t25 Phobius SIGNAL_PEPTIDE Signal peptide region 1 22 -
15 g4405.t25 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 5 -
16 g4405.t25 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 6 17 -
17 g4405.t25 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 18 22 -
13 g4405.t25 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 23 492 -
21 g4405.t25 ProSitePatterns PS00139 Eukaryotic thiol (cysteine) proteases cysteine active site. 401 412 -
25 g4405.t25 SMART SM00043 CY_4 34 121 7.0E-10
24 g4405.t25 SMART SM00043 CY_4 145 234 0.18
23 g4405.t25 SMART SM00848 Inhibitor_I29_2 297 354 3.0E-18
22 g4405.t25 SMART SM00645 pept_c1 383 492 7.3E-10
6 g4405.t25 SUPERFAMILY SSF54403 Cystatin/monellin 32 123 1.04E-13
7 g4405.t25 SUPERFAMILY SSF54403 Cystatin/monellin 155 222 3.19E-8
8 g4405.t25 SUPERFAMILY SSF54001 Cysteine proteinases 290 491 2.0E-61
9 g4405.t25 SignalP_EUK SignalP-noTM SignalP-noTM 1 19 -
20 g4405.t25 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM 1 19 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0008234 cysteine-type peptidase activity MF
GO:0006508 proteolysis BP
GO:0004869 cysteine-type endopeptidase inhibitor activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values