General information for g4405.t27
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative cysteine proteinase CG12163.
Parent gene
- The parent gene g4405 is located on chr_2: 2005157..2008641 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4405 | g4405.t27 | TTS | g4405.t27 | 2001061 | 2001061 |
| chr_2 | g4405 | g4405.t27 | isoform | g4405.t27 | 2001868 | 2002971 |
| chr_2 | g4405 | g4405.t27 | exon | g4405.t27.exon1 | 2001868 | 2002007 |
| chr_2 | g4405 | g4405.t27 | cds | g4405.t27.CDS1 | 2001869 | 2002007 |
| chr_2 | g4405 | g4405.t27 | exon | g4405.t27.exon2 | 2002061 | 2002971 |
| chr_2 | g4405 | g4405.t27 | cds | g4405.t27.CDS2 | 2002061 | 2002431 |
| chr_2 | g4405 | g4405.t27 | TSS | g4405.t27 | NA | NA |
Sequences
>g4405.t27 Gene=g4405 Length=1051
GCTTGGATTAAAGATGGACGTAAAGTTTCAGTTTCTTGCAAAAATGATAAGAAATATAAG
TTGACCCAATCACCATCTAATCAGCGTGTCAAACGTGATAACACGCTTGAAAGAGTTCTT
GGTTTACCATCCAATACTGATGATCATGACGATTTGATAAAAATACTTTCTGAACATTTG
AAGAGACTCGATACTGGAAGTGATGCACAATTTGAATTGGTAAAACTTGAAAAGGTAACT
CAACAAGTAGTAGCTGGAATAAAATATAAAGCAACAGGTATTTTTAAAATTGGCAATGAA
GAGAAAAAATGTGTTATCGATGTATGGCATCGCTCATGGATTAAGGGAGATGAAGGCACT
CAATTAAGCGCTGATTGTGATAAAGGTGCAACAACTTTCAAGACAAAATCTTCTAGAAAA
AGGAGATCAGTTCATCACCACACACACAATCGTCACAATAGACAATCAGTAAGCGATCAT
TTTGATGACCATCATCATCATACTGATAGACATCATCATCAATACTCAGCTACTGAAGAA
ATGAAAGAAATAAAATCTGAAATTTTATTTAACAATTTCATAACTAAATATAATCGTAAA
TATGCCAATGAACTTGAACATAAAATGAGAATGAGAATTTTCAAGAAGAATTTACATAAA
ATTGAAATGTTGAATAAGCATGAACAAGGCACTGCAAAGTATGGAATTACAGAATTCGCT
GATTTAACTGAAAAGGAATACTTGCATAAAACTGGTTTGAGAGTGCGTGAAAGACATGAG
AATGAATTAGAAAATCCAATTGCACATATTCCAGAAGTTGAAGATTTACCAACCGAATTT
GATTGGAGAGATAAATCAGCAGTTACAAGTGTAAAAAATCAAGGAAATTGTGGATCATGC
TGGAGTTTTTCTGTTACAGGAAATATTGAAGGCTTACATGCTATTAAAACTGGAAAACTT
GAAGCTTATTCTGAACAAGAACTTTTGGACTGTGATACAACTGATAATGCTTGCAATGGT
GGTTATATGGATGATGCTTTTAAAGCAATTG
>g4405.t27 Gene=g4405 Length=170
MKEIKSEILFNNFITKYNRKYANELEHKMRMRIFKKNLHKIEMLNKHEQGTAKYGITEFA
DLTEKEYLHKTGLRVRERHENELENPIAHIPEVEDLPTEFDWRDKSAVTSVKNQGNCGSC
WSFSVTGNIEGLHAIKTGKLEAYSEQELLDCDTTDNACNGGYMDDAFKAI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g4405.t27 | CDD | cd02248 | Peptidase_C1A | 97 | 170 | 9.64222E-43 |
| 9 | g4405.t27 | Gene3D | G3DSA:3.90.70.10 | Cysteine proteinases | 1 | 170 | 6.1E-55 |
| 3 | g4405.t27 | PANTHER | PTHR13814:SF16 | CYSTATIN | 9 | 170 | 4.4E-64 |
| 4 | g4405.t27 | PANTHER | PTHR13814 | FETUIN | 9 | 170 | 4.4E-64 |
| 1 | g4405.t27 | Pfam | PF08246 | Cathepsin propeptide inhibitor domain (I29) | 10 | 67 | 4.5E-13 |
| 2 | g4405.t27 | Pfam | PF00112 | Papain family cysteine protease | 96 | 169 | 1.6E-28 |
| 8 | g4405.t27 | ProSitePatterns | PS00139 | Eukaryotic thiol (cysteine) proteases cysteine active site. | 114 | 125 | - |
| 7 | g4405.t27 | SMART | SM00848 | Inhibitor_I29_2 | 10 | 67 | 3.0E-18 |
| 6 | g4405.t27 | SMART | SM00645 | pept_c1 | 96 | 170 | 5.1E-4 |
| 5 | g4405.t27 | SUPERFAMILY | SSF54001 | Cysteine proteinases | 7 | 170 | 9.47E-55 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008234 | cysteine-type peptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
