General information for g4433.t14
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Ectonucleoside triphosphate diphosphohydrolase 5.
Parent gene
- The parent gene g4433 is located on chr_2: 2138905..2140409 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4433 | g4433.t14 | TSS | g4433.t14 | 2137054 | 2137054 |
| chr_2 | g4433 | g4433.t14 | isoform | g4433.t14 | 2137761 | 2139213 |
| chr_2 | g4433 | g4433.t14 | exon | g4433.t14.exon1 | 2137761 | 2137790 |
| chr_2 | g4433 | g4433.t14 | cds | g4433.t14.CDS1 | 2137761 | 2137790 |
| chr_2 | g4433 | g4433.t14 | exon | g4433.t14.exon2 | 2137902 | 2138044 |
| chr_2 | g4433 | g4433.t14 | cds | g4433.t14.CDS2 | 2137902 | 2138044 |
| chr_2 | g4433 | g4433.t14 | exon | g4433.t14.exon3 | 2138501 | 2138592 |
| chr_2 | g4433 | g4433.t14 | cds | g4433.t14.CDS3 | 2138501 | 2138592 |
| chr_2 | g4433 | g4433.t14 | exon | g4433.t14.exon4 | 2138662 | 2139213 |
| chr_2 | g4433 | g4433.t14 | cds | g4433.t14.CDS4 | 2138662 | 2139212 |
| chr_2 | g4433 | g4433.t14 | TTS | g4433.t14 | 2140138 | 2140138 |
Sequences
>g4433.t14 Gene=g4433 Length=817
ATGAAACATTGTGAAGACGAATTTGAACTTCTCATTCAAGAGAATGGTGATAAACAGGGA
ATAAGAAGAAAGATTCATGAAAAGCACAATAATAAAGCAAATCAAAATGCAGGACTGGTT
ACTAGTTTCTTATGTTTGGTCGCTACTGTAACGATATTAATAATATTTTTCAGTGTTTAT
ACTGATTCACTACATCCCATTGTCGATCAGATTGCTACACAGCTTGGCTATTCAGAAAAG
CAATATGGTGCAATTATTGATGCTGGCTCAACTGGCAGTCGTGTGTTAGCTTTTGAATTT
CATAAAGCATATCTTGATGGACGACTTGTATTAGATAACGAATTATTTAGAGAATTGAAG
CCCGGACTATCCTCTTTGACACCGGACAAAGGTGCAGAACAAATTACAAGATTACTAAAG
GAGGCTAAAGATTTTATTCCTGCCGAATATAGAAGAAAAACTCCTATCGCTTTAAAAGCT
ACTGCCGGCTTAAGATTATTAGGTGCAGCAAAATCTGAAGCAATTCTAGAAAAAATTCGT
GAAGTTTTTGCACAATCTGGATTTTTAATCAACGATGATTCAGTAGAGATTATGGATGGA
ACTGATGAAGGCATATTCTCATGGTTCACAGTCAATTATCTCTCAAATCGACTGACATCA
CGTAACACTGCAGCTGCATTAGATTTAGGTGGAGGTTCTACACAAGTGACTTACGAATTA
AACGAGAATTTGCCAAGCTATAAAGAATATATTCACACTGTGCCTGTACTCAATTCTGAA
TTGAATGTTTTTACTAACTCCTATCTTGGTTTAGGTT
>g4433.t14 Gene=g4433 Length=272
MKHCEDEFELLIQENGDKQGIRRKIHEKHNNKANQNAGLVTSFLCLVATVTILIIFFSVY
TDSLHPIVDQIATQLGYSEKQYGAIIDAGSTGSRVLAFEFHKAYLDGRLVLDNELFRELK
PGLSSLTPDKGAEQITRLLKEAKDFIPAEYRRKTPIALKATAGLRLLGAAKSEAILEKIR
EVFAQSGFLINDDSVEIMDGTDEGIFSWFTVNYLSNRLTSRNTAAALDLGGGSTQVTYEL
NENLPSYKEYIHTVPVLNSELNVFTNSYLGLG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g4433.t14 | CDD | cd00012 | NBD_sugar-kinase_HSP70_actin | 84 | 241 | 1.16719E-5 |
| 6 | g4433.t14 | Gene3D | G3DSA:3.30.420.40 | - | 70 | 200 | 6.1E-25 |
| 5 | g4433.t14 | Gene3D | G3DSA:3.30.420.150 | Exopolyphosphatase. Domain 2 | 201 | 272 | 7.8E-23 |
| 2 | g4433.t14 | PANTHER | PTHR11782:SF83 | NTPASE, ISOFORM F | 44 | 272 | 1.4E-57 |
| 3 | g4433.t14 | PANTHER | PTHR11782 | ADENOSINE/GUANOSINE DIPHOSPHATASE | 44 | 272 | 1.4E-57 |
| 1 | g4433.t14 | Pfam | PF01150 | GDA1/CD39 (nucleoside phosphatase) family | 78 | 272 | 4.9E-48 |
| 7 | g4433.t14 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 36 | - |
| 9 | g4433.t14 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 37 | 60 | - |
| 8 | g4433.t14 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 61 | 272 | - |
| 4 | g4433.t14 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 38 | 60 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01510 (apyrase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed