General information for g4433.t20
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Ectonucleoside triphosphate diphosphohydrolase 5.
Parent gene
- The parent gene g4433 is located on chr_2: 2138905..2140409 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4433 | g4433.t20 | TSS | g4433.t20 | 2138427 | 2138427 |
| chr_2 | g4433 | g4433.t20 | isoform | g4433.t20 | 2138473 | 2139921 |
| chr_2 | g4433 | g4433.t20 | exon | g4433.t20.exon1 | 2138473 | 2138592 |
| chr_2 | g4433 | g4433.t20 | exon | g4433.t20.exon2 | 2138662 | 2139540 |
| chr_2 | g4433 | g4433.t20 | cds | g4433.t20.CDS1 | 2138988 | 2139540 |
| chr_2 | g4433 | g4433.t20 | exon | g4433.t20.exon3 | 2139604 | 2139921 |
| chr_2 | g4433 | g4433.t20 | cds | g4433.t20.CDS2 | 2139604 | 2139839 |
| chr_2 | g4433 | g4433.t20 | TTS | g4433.t20 | 2140138 | 2140138 |
Sequences
>g4433.t20 Gene=g4433 Length=1317
TAGTTTTTTTAATATCTTTTATTTTTAGTGTTTATACTGATTCACTACATCCCATTGTCG
ATCAGATTGCTACACAGCTTGGCTATTCAGAAAAGCAATATGGTGCAATTATTGATGCTG
GCTCAACTGGCAGTCGTGTGTTAGCTTTTGAATTTCATAAAGCATATCTTGATGGACGAC
TTGTATTAGATAACGAATTATTTAGAGAATTGAAGCCCGGACTATCCTCTTTGACACCGG
ACAAAGGTGCAGAACAAATTACAAGATTACTAAAGGAGGCTAAAGATTTTATTCCTGCCG
AATATAGAAGAAAAACTCCTATCGCTTTAAAAGCTACTGCCGGCTTAAGATTATTAGGTG
CAGCAAAATCTGAAGCAATTCTAGAAAAAATTCGTGAAGTTTTTGCACAATCTGGATTTT
TAATCAACGATGATTCAGTAGAGATTATGGATGGAACTGATGAAGGCATATTCTCATGGT
TCACAGTCAATTATCTCTCAAATCGACTGACATCACGTAACACTGCAGCTGCATTAGATT
TAGGTGGAGGTTCTACACAAGTGACTTACGAATTAAACGAGAATTTGCCAAGCTATAAAG
AATATATTCACACTGTGCCTGTACTCAATTCTGAATTGAATGTTTTTACTAACTCCTATC
TTGGTTTAGGTTTGATGGCGTTACGTCATGCTGTAATTACTTATCAAAAGGCTGATAATC
AAACATCATTTGCAAGTGAATGTATTAATCCCATTATAAAGGATAAAGTTTGGACCTATC
AAAATATCGACTACCTTGTATCTGGAAAAGAAAATAAAAATCAAGCCTCAAATCCAGAAA
ACCCCGAAGTAAATTTTGACCTTTGTGTAGAGTCAGTAAAGCGACAAGTAATGCCATTAT
TAAAACCAAAACCAATTAAATTGCAAGAGCACGTGATTAATGCGTTTTCCTATTATTATG
ATCGTGCCATTGAAGTTGGTTTGGTTGATCCATATTTAGGAGGTGAAAATACTGTTGCAG
ATTTCTCTAAAAAGGCTCGTGAAGTCTGTGCAACAGCTAATGCTGATCAACCATTTATGT
GCTTAGATTTGGTTTACATATCAGTATTACTTCAAGATGGATTCGGTCTCAAACCACAAA
CAAATATAAAGGTATACTGTAATTGTTCATATTATTTAACATTCGTAGTAAATCTTATTA
TATTTATAGCTTTACAAAAAAATAAGAGGACATGAAGTTTCGTGGGCTTTGGGCTGTTTC
TTTCAAAATTATAATATCCTACAGCAAAAAATGAAACGAGTAGCTGGTGTGAATTAG
>g4433.t20 Gene=g4433 Length=262
MDGTDEGIFSWFTVNYLSNRLTSRNTAAALDLGGGSTQVTYELNENLPSYKEYIHTVPVL
NSELNVFTNSYLGLGLMALRHAVITYQKADNQTSFASECINPIIKDKVWTYQNIDYLVSG
KENKNQASNPENPEVNFDLCVESVKRQVMPLLKPKPIKLQEHVINAFSYYYDRAIEVGLV
DPYLGGENTVADFSKKAREVCATANADQPFMCLDLVYISVLLQDGFGLKPQTNIKVYCNC
SYYLTFVVNLIIFIALQKNKRT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g4433.t20 | Gene3D | G3DSA:3.30.420.150 | Exopolyphosphatase. Domain 2 | 2 | 242 | 2.5E-33 |
| 2 | g4433.t20 | PANTHER | PTHR11782:SF35 | ECTONUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 5 | 1 | 240 | 1.1E-46 |
| 3 | g4433.t20 | PANTHER | PTHR11782 | ADENOSINE/GUANOSINE DIPHOSPHATASE | 1 | 240 | 1.1E-46 |
| 1 | g4433.t20 | Pfam | PF01150 | GDA1/CD39 (nucleoside phosphatase) family | 1 | 235 | 5.5E-24 |
| 7 | g4433.t20 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 240 | - |
| 8 | g4433.t20 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 241 | 257 | - |
| 6 | g4433.t20 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 258 | 262 | - |
| 4 | g4433.t20 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 234 | 256 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
