Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Mitochondrial fission 1 protein.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g4525 g4525.t1 TSS g4525.t1 3058998 3058998
chr_2 g4525 g4525.t1 isoform g4525.t1 3059065 3059710
chr_2 g4525 g4525.t1 exon g4525.t1.exon1 3059065 3059109
chr_2 g4525 g4525.t1 cds g4525.t1.CDS1 3059065 3059109
chr_2 g4525 g4525.t1 exon g4525.t1.exon2 3059184 3059413
chr_2 g4525 g4525.t1 cds g4525.t1.CDS2 3059184 3059413
chr_2 g4525 g4525.t1 exon g4525.t1.exon3 3059475 3059532
chr_2 g4525 g4525.t1 cds g4525.t1.CDS3 3059475 3059532
chr_2 g4525 g4525.t1 exon g4525.t1.exon4 3059594 3059710
chr_2 g4525 g4525.t1 cds g4525.t1.CDS4 3059594 3059710
chr_2 g4525 g4525.t1 TTS g4525.t1 3059886 3059886

Sequences

>g4525.t1 Gene=g4525 Length=450
ATGGAGGAAATGTTAAATGTTACAGTGACAGCACAAGAATTAGAAAAATATGAAAAGATT
TATATGAGAGAATTATCTGAAAACAAAAAAGCTTCAAATAAAACACAATTCGATTTTGCA
AGTTGCTTGGTACGTTCTAAATATGGTGCAGATATTCATAATGGAATAAAAATGTTCGAG
AACTTGAGTAAAGAAGATCCAGAAAATAAACGAGATTATATTTATTTCATTGCCATTGCT
TATACTAGATTGAAAGATTGGCAAACAGCTCACAAGTATGTGAAAACTTTCTTAGAAATC
GAACCAAATAATTTACAAGTTTCGTATTTAAATGAACATATTGAAAAAGAAATGAATAAA
GAACTGAAAAAAGAAGTAGCTCTCGCAGGAAGCGTAATATTGGGAATTGGAGCAGTAGCA
GGACTTGCATTCGCGCTTGCTAAAAAGTAG

>g4525.t1 Gene=g4525 Length=149
MEEMLNVTVTAQELEKYEKIYMRELSENKKASNKTQFDFASCLVRSKYGADIHNGIKMFE
NLSKEDPENKRDYIYFIAIAYTRLKDWQTAHKYVKTFLEIEPNNLQVSYLNEHIEKEMNK
ELKKEVALAGSVILGIGAVAGLAFALAKK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
13 g4525.t1 CDD cd12212 Fis1 8 121 3.52949E-36
8 g4525.t1 Coils Coil Coil 14 34 -
7 g4525.t1 Coils Coil Coil 146 149 -
6 g4525.t1 Gene3D G3DSA:1.25.40.10 - 1 147 2.0E-39
3 g4525.t1 PANTHER PTHR13247 TETRATRICOPEPTIDE REPEAT PROTEIN 11 TPR REPEAT PROTEIN 11 7 146 3.4E-28
4 g4525.t1 PANTHER PTHR13247:SF0 MITOCHONDRIAL FISSION 1 PROTEIN 7 146 3.4E-28
12 g4525.t1 PIRSF PIRSF008835 TPR_repeat_11_Fis1 1 149 1.3E-39
2 g4525.t1 Pfam PF14852 Fis1 N-terminal tetratricopeptide repeat 35 65 1.8E-7
1 g4525.t1 Pfam PF14853 Fis1 C-terminal tetratricopeptide repeat 71 121 4.7E-18
9 g4525.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 125 -
11 g4525.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 126 147 -
10 g4525.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 148 149 -
5 g4525.t1 SUPERFAMILY SSF48452 TPR-like 1 148 1.87E-33
14 g4525.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 125 147 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005515 protein binding MF
GO:0000266 mitochondrial fission BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values