Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Mitochondrial fission 1 protein.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g4525 g4525.t5 TSS g4525.t5 3058998 3058998
chr_2 g4525 g4525.t5 isoform g4525.t5 3059184 3059710
chr_2 g4525 g4525.t5 exon g4525.t5.exon1 3059184 3059413
chr_2 g4525 g4525.t5 cds g4525.t5.CDS1 3059202 3059413
chr_2 g4525 g4525.t5 exon g4525.t5.exon2 3059475 3059532
chr_2 g4525 g4525.t5 cds g4525.t5.CDS2 3059475 3059532
chr_2 g4525 g4525.t5 exon g4525.t5.exon3 3059594 3059710
chr_2 g4525 g4525.t5 cds g4525.t5.CDS3 3059594 3059710
chr_2 g4525 g4525.t5 TTS g4525.t5 3059886 3059886

Sequences

>g4525.t5 Gene=g4525 Length=405
AAATATGAAAAGATTTATATGAGAGAATTATCTGAAAACAAAAAAGCTTCAAATAAAACA
CAATTCGATTTTGCAAGTTGCTTGGTACGTTCTAAATATGGTGCAGATATTCATAATGGA
ATAAAAATGTTCGAGAACTTGAGTAAAGAAGATCCAGAAAATAAACGAGATTATATTTAT
TTCATTGCCATTGCTTATACTAGATTGAAAGATTGGCAAACAGCTCACAAGTATGTGAAA
ACTTTCTTAGAAATCGAACCAAATAATTTACAAGTTTCGTATTTAAATGAACATATTGAA
AAAGAAATGAATAAAGAACTGAAAAAAGAAGTAGCTCTCGCAGGAAGCGTAATATTGGGA
ATTGGAGCAGTAGCAGGACTTGCATTCGCGCTTGCTAAAAAGTAG

>g4525.t5 Gene=g4525 Length=128
MRELSENKKASNKTQFDFASCLVRSKYGADIHNGIKMFENLSKEDPENKRDYIYFIAIAY
TRLKDWQTAHKYVKTFLEIEPNNLQVSYLNEHIEKEMNKELKKEVALAGSVILGIGAVAG
LAFALAKK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
11 g4525.t5 CDD cd12212 Fis1 10 100 4.6205E-33
7 g4525.t5 Gene3D G3DSA:1.25.40.10 - 1 126 2.5E-34
3 g4525.t5 PANTHER PTHR13247 TETRATRICOPEPTIDE REPEAT PROTEIN 11 TPR REPEAT PROTEIN 11 6 125 2.9E-25
4 g4525.t5 PANTHER PTHR13247:SF0 MITOCHONDRIAL FISSION 1 PROTEIN 6 125 2.9E-25
2 g4525.t5 Pfam PF14852 Fis1 N-terminal tetratricopeptide repeat 14 44 1.4E-7
1 g4525.t5 Pfam PF14853 Fis1 C-terminal tetratricopeptide repeat 50 100 3.4E-18
8 g4525.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 104 -
10 g4525.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 105 126 -
9 g4525.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 127 128 -
6 g4525.t5 SUPERFAMILY SSF48452 TPR-like 8 127 9.11E-30
5 g4525.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 104 126 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005515 protein binding MF
GO:0000266 mitochondrial fission BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values