General information for g4583.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Hormone-sensitive lipase.
Parent gene
- The parent gene g4583 is located on chr_2: 3329847..3333453 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4583 | g4583.t1 | TTS | g4583.t1 | 3328253 | 3328253 |
| chr_2 | g4583 | g4583.t1 | isoform | g4583.t1 | 3328506 | 3333453 |
| chr_2 | g4583 | g4583.t1 | exon | g4583.t1.exon1 | 3328506 | 3328592 |
| chr_2 | g4583 | g4583.t1 | cds | g4583.t1.CDS1 | 3328506 | 3328592 |
| chr_2 | g4583 | g4583.t1 | exon | g4583.t1.exon2 | 3328707 | 3329450 |
| chr_2 | g4583 | g4583.t1 | cds | g4583.t1.CDS2 | 3328707 | 3329450 |
| chr_2 | g4583 | g4583.t1 | exon | g4583.t1.exon3 | 3329520 | 3329738 |
| chr_2 | g4583 | g4583.t1 | cds | g4583.t1.CDS3 | 3329520 | 3329738 |
| chr_2 | g4583 | g4583.t1 | exon | g4583.t1.exon4 | 3329816 | 3329847 |
| chr_2 | g4583 | g4583.t1 | cds | g4583.t1.CDS4 | 3329816 | 3329847 |
| chr_2 | g4583 | g4583.t1 | exon | g4583.t1.exon5 | 3329896 | 3330200 |
| chr_2 | g4583 | g4583.t1 | cds | g4583.t1.CDS5 | 3329896 | 3330200 |
| chr_2 | g4583 | g4583.t1 | exon | g4583.t1.exon6 | 3333095 | 3333453 |
| chr_2 | g4583 | g4583.t1 | cds | g4583.t1.CDS6 | 3333095 | 3333453 |
| chr_2 | g4583 | g4583.t1 | TSS | g4583.t1 | 3333525 | 3333525 |
Sequences
>g4583.t1 Gene=g4583 Length=1746
ATGAAGTCAAAGGAGTTGAAAAAATTTGCAAATTTAAAGTTCAGTTCACATTCGATTGAA
GTCGATCAATTGATATTAAGCGTTCTGGACAGCATTGAATTTTTTAAAACACAAAAGGAC
TCATCACAACTTTCAAATGGCCTCCAGCAATTTAATTTGCATCTCGAGAAATTATCAAAA
ATTGCCAACACTCTTGAAAATGCAGCACGCGAATGTGATTATGACATTAATTCACCGGGT
AATGGATATTGGAGTTATATTCATAGTTTCAATTGTGCCATTAAAATAATGCAAAATACA
TTCAAGCAAATGACCAAAAATCGTGAGAAAATCCTTTTCAACAAAAAATTTTACATCAAA
GAACTTGACCTTTGGAATCAAATTTTCGAGAGTCTTATTGAAATTTGTGAAACACTATTA
CAATTGCGTGAAGGCGGTGATAAGACGGTTTTATTTTCGCAAGACAACAATTGGGTCATT
CTTAATCGTCTTCATTCAACAATTCATCAAAAAGCATTCTATGGCAGTGCACTTGGATTT
CAATGTGCAAAATCTGTGAAGCCACTTGTCAATTTTCTGGCCTCAACTCTCGTCAGTTAC
TCAAAATGGCATTACTCTGATAAACACAAATCAATCAAACTTCTTGATTTTCCTCTTTCA
ACCGCTTCAAACAAGGGCGATGTAGACTTTTGCAAGAGCTTCTGGTTTATGTCTGAGTCA
CCTGCTATGCATACTTTACCAAATGCTTTAAGCGATAGCATGGACATTAACAAAATTTTC
AAAATACCAGCTGAACCTTTAAAAGTATTCAGTGAGAAGTTCCAACAGCACATCGATATT
CCACTTCCGCATAGCCACATTGGAATTCAACCAGTATCTTGCCGTCTTCTTTGTGCACGG
AAACGTGAGGGAATGCTGGGCAAAAAATCGTCATCGAAATCACTGCCAAGGTCAAGAAAT
CTTCTTTTTCATGTTCATGGTGGAGGATGGGCAGCACAAACATCAAAATCACATGAAGTG
TACCTGAGACAATGGGCATCATATCTTGATTGTCCTATTTTATCTATTGATTATCAACTT
GCACCTAAAGCACCATTTCCAAGAGGATTGGAAGATATTTATTACGCCTACTGTTGGGCA
TTGAACAATTTTGAATACCTTGGAACAACTGGAGAAAAGATTGTATTTGCAGGTGATAGT
GCTGGTGCAAATTTGAATTCTGCCCTCATGATCAAATGCATCGAAAATGGTGTGCGAATT
CCTGACGGTCTACTGAACATTTATGGAATTTATCACGTCGATTTTCTTATTGCTCCATCA
GTGTGCCTTTCACTAACTGATCCAATGCTTCCTTTTGGTTTAACATCAAATTTAATCAAA
TGTTATGGAATAGATAAAAATGTTATAAATACCGACATTAATATGAACATTATTGGAGAA
AAAGACATCTTTAAATTTCATGACAGCTATTTATTATCACCTTATAAAGCACCTGAAGAA
ATTCTTAAACAATTTCCACCAACAAGATTTTTATCATCAATTCTTGATCCTCTATTGGAT
GATAATATTGAATTTGGGAAGAAATTGAGAAATTTAGGAGTAAATACAAATGTTGATATT
TTACAGGGATTATATCATGGATTTCTGTATTTTATTAAAATATCTAAAGATTGCCGCGAC
GGTTCACTTATGTGTGCTGCACGCATTAAAGAACTTTTTGAGATGCAACAACAGCAACAG
CATTGA
>g4583.t1 Gene=g4583 Length=581
MKSKELKKFANLKFSSHSIEVDQLILSVLDSIEFFKTQKDSSQLSNGLQQFNLHLEKLSK
IANTLENAARECDYDINSPGNGYWSYIHSFNCAIKIMQNTFKQMTKNREKILFNKKFYIK
ELDLWNQIFESLIEICETLLQLREGGDKTVLFSQDNNWVILNRLHSTIHQKAFYGSALGF
QCAKSVKPLVNFLASTLVSYSKWHYSDKHKSIKLLDFPLSTASNKGDVDFCKSFWFMSES
PAMHTLPNALSDSMDINKIFKIPAEPLKVFSEKFQQHIDIPLPHSHIGIQPVSCRLLCAR
KREGMLGKKSSSKSLPRSRNLLFHVHGGGWAAQTSKSHEVYLRQWASYLDCPILSIDYQL
APKAPFPRGLEDIYYAYCWALNNFEYLGTTGEKIVFAGDSAGANLNSALMIKCIENGVRI
PDGLLNIYGIYHVDFLIAPSVCLSLTDPMLPFGLTSNLIKCYGIDKNVINTDINMNIIGE
KDIFKFHDSYLLSPYKAPEEILKQFPPTRFLSSILDPLLDDNIEFGKKLRNLGVNTNVDI
LQGLYHGFLYFIKISKDCRDGSLMCAARIKELFEMQQQQQH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g4583.t1 | Coils | Coil | Coil | 48 | 68 | - |
| 7 | g4583.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 307 | 577 | 2.2E-49 |
| 4 | g4583.t1 | PANTHER | PTHR23025 | TRIACYLGLYCEROL LIPASE | 28 | 578 | 2.4E-130 |
| 1 | g4583.t1 | Pfam | PF06350 | Hormone-sensitive lipase (HSL) N-terminus | 28 | 210 | 1.5E-29 |
| 2 | g4583.t1 | Pfam | PF06350 | Hormone-sensitive lipase (HSL) N-terminus | 221 | 305 | 1.7E-24 |
| 3 | g4583.t1 | Pfam | PF07859 | alpha/beta hydrolase fold | 323 | 549 | 3.9E-35 |
| 6 | g4583.t1 | ProSitePatterns | PS01173 | Lipolytic enzymes G-D-X-G family, putative histidine active site. | 322 | 338 | - |
| 5 | g4583.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 319 | 552 | 2.04E-39 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016042 | lipid catabolic process | BP |
| GO:0016787 | hydrolase activity | MF |
| GO:0008203 | cholesterol metabolic process | BP |
| GO:0016298 | lipase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01066 (acetyl esterase [EC:3.1.1.-])
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
