General information for g4589.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 6a20.
Parent gene
- The parent gene g4589 is located on chr_2: 3352032..3353091 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4589 | g4589.t2 | TTS | g4589.t2 | 3351404 | 3351404 |
| chr_2 | g4589 | g4589.t2 | isoform | g4589.t2 | 3351505 | 3353091 |
| chr_2 | g4589 | g4589.t2 | exon | g4589.t2.exon1 | 3351505 | 3351681 |
| chr_2 | g4589 | g4589.t2 | exon | g4589.t2.exon2 | 3351737 | 3353091 |
| chr_2 | g4589 | g4589.t2 | cds | g4589.t2.CDS1 | 3351988 | 3353091 |
| chr_2 | g4589 | g4589.t2 | TSS | g4589.t2 | 3353129 | 3353129 |
Sequences
>g4589.t2 Gene=g4589 Length=1532
ATGGAGCTAACAACGCTTCTTTTTACAATTTTTACCGGAATTTCGTTATTTATTTACTAT
TTTATACATCGTCGTTTAAATTATTGGAAGGTCAGAAATGTACCACACATTGAACCAGAA
TTTTTCCATGGAAACGCTCGTGGTGTCGGCAAAACAATATCTCCTGGTAAATTTTTCAAG
GACATGTACTTTAAACTAAAGAATAAAGGTCCAGTAGTTGGCGTATACATGTATGTAGAA
CCAATGGCAATAATAATGGATTTAGATTTAGTGAAAACAATTCTCGTGAAAGAATTTAAT
ACTTTTCCAAATCGTGGACTTTATCATAATGAAAAAGATGATCCAACAAGTGCAAATTTA
GCATCAATTGAAGATGAACAGTGGAAAATTTTAAGAAATAAAATATCACCAACATTCTCC
AGTGGAAAAATTAAAAACATGTTTCCAATAGTAGGTGAAGTAGCTGATAAAATGATTGAA
CGTATTGAAATTGATAGCAAAGAGAAAGGCTCGATTGAAGTGAAAGATATTTTAGCGAGA
TTTACAACAGATGTGATTGGAAGAGTTGGATTTGGAATAGAGTGCAATAGTTTAGATGAT
AACACAACAAAATTCTATCAAATTGGTTTAAAAGCGTTCAGTAATATAAATTTTTTCACA
AGAACTTTGACATCATCTTATCCAGATTTAGCAAGAAAATTACACATTAAAACATCTGAT
CCAGAAGTTGCTGGCTTTTATAAAGATATTGTTGAGCAAACAATCAAATATCGTATAGAT
AATAATATTCAGAGCAATGACTTTATGTCACTTTTAATGAAGATGATGAAAGATAACTCT
TTGACCTTTAATGAAGTCTGGTCACAATCAGTAGTTTTCTTTCTAGCTGGTTATGAAACA
AGTTCTACAGCTTTAAATTATTGTTTATATGAAATGTCTCAAAGAAAAGACATTTGTGAT
AAAGCAAGAGAATCAGTGAAAAAAGCATTAGAAAAACATAACGGAGAATTAAATTATGAT
GCTGTCAATGACATGCAATATATTGAACAATGTGTTAATGGTAAATATTTTTCTTTAAAA
AAAGAGTTTAAAATAAGATTATAAAATTTTTAGAGGCTTTAAGAAAACATCCGCCAGCTA
ATGCTACAAGAAGAATTCCAAGAGAAGATTTTCAAGTGCCAAATAGTAAAATTGTGATTG
AAAAAGGAGTTCATGTAATTATTCCAGTTTGGGGAATCCATTTCGATCCAGAAATCTATC
CAGAACCAGAAACTTATAATCCAGACAGATTTACTCCTGAAGAAGTAGCTAAAAGACATA
ACATGAGCTTTCTACCATTCGGTGAAGGCCCAAGAGTTTGCATTGGTGAAAGATTTGCTA
TGGTCGAAATTAAATTAGCTTTAGCAAAAATTCTCATGAATTTTGAATTTGAGCTTGACT
ACTCAAAAACTCCAGTTCCAATGCCTTATGAAACCAAGAGACTAATTTTGACGGCAGCTA
AAGGAGTTTTTATAAAATTTAGGAAAATTTAA
>g4589.t2 Gene=g4589 Length=367
MELTTLLFTIFTGISLFIYYFIHRRLNYWKVRNVPHIEPEFFHGNARGVGKTISPGKFFK
DMYFKLKNKGPVVGVYMYVEPMAIIMDLDLVKTILVKEFNTFPNRGLYHNEKDDPTSANL
ASIEDEQWKILRNKISPTFSSGKIKNMFPIVGEVADKMIERIEIDSKEKGSIEVKDILAR
FTTDVIGRVGFGIECNSLDDNTTKFYQIGLKAFSNINFFTRTLTSSYPDLARKLHIKTSD
PEVAGFYKDIVEQTIKYRIDNNIQSNDFMSLLMKMMKDNSLTFNEVWSQSVVFFLAGYET
SSTALNYCLYEMSQRKDICDKARESVKKALEKHNGELNYDAVNDMQYIEQCVNGKYFSLK
KEFKIRL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g4589.t2 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 17 | 353 | 4.5E-75 |
| 2 | g4589.t2 | PANTHER | PTHR24292 | CYTOCHROME P450 | 7 | 353 | 1.3E-110 |
| 3 | g4589.t2 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 7 | 353 | 1.3E-110 |
| 5 | g4589.t2 | PRINTS | PR00464 | Group II E-class P450 signature | 125 | 145 | 2.8E-8 |
| 4 | g4589.t2 | PRINTS | PR00464 | Group II E-class P450 signature | 180 | 198 | 2.8E-8 |
| 1 | g4589.t2 | Pfam | PF00067 | Cytochrome P450 | 51 | 353 | 9.4E-48 |
| 10 | g4589.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 11 | g4589.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 22 | - |
| 9 | g4589.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 23 | 367 | - |
| 7 | g4589.t2 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 41 | 353 | 1.83E-58 |
| 6 | g4589.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0004497 | monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
