General information for g4612.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chitotriosidase-1.
Parent gene
- The parent gene g4612 is located on chr_2: 3584855..3585990 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4612 | g4612.t1 | TSS | g4612.t1 | 3580889 | 3580889 |
| chr_2 | g4612 | g4612.t1 | isoform | g4612.t1 | 3581014 | 3584855 |
| chr_2 | g4612 | g4612.t1 | exon | g4612.t1.exon1 | 3581014 | 3581077 |
| chr_2 | g4612 | g4612.t1 | cds | g4612.t1.CDS1 | 3581014 | 3581077 |
| chr_2 | g4612 | g4612.t1 | exon | g4612.t1.exon2 | 3582610 | 3582638 |
| chr_2 | g4612 | g4612.t1 | cds | g4612.t1.CDS2 | 3582610 | 3582638 |
| chr_2 | g4612 | g4612.t1 | exon | g4612.t1.exon3 | 3582696 | 3582825 |
| chr_2 | g4612 | g4612.t1 | cds | g4612.t1.CDS3 | 3582696 | 3582825 |
| chr_2 | g4612 | g4612.t1 | exon | g4612.t1.exon4 | 3583126 | 3583644 |
| chr_2 | g4612 | g4612.t1 | cds | g4612.t1.CDS4 | 3583126 | 3583644 |
| chr_2 | g4612 | g4612.t1 | exon | g4612.t1.exon5 | 3583706 | 3584065 |
| chr_2 | g4612 | g4612.t1 | cds | g4612.t1.CDS5 | 3583706 | 3584065 |
| chr_2 | g4612 | g4612.t1 | exon | g4612.t1.exon6 | 3584124 | 3584188 |
| chr_2 | g4612 | g4612.t1 | cds | g4612.t1.CDS6 | 3584124 | 3584188 |
| chr_2 | g4612 | g4612.t1 | exon | g4612.t1.exon7 | 3584244 | 3584855 |
| chr_2 | g4612 | g4612.t1 | cds | g4612.t1.CDS7 | 3584244 | 3584855 |
| chr_2 | g4612 | g4612.t1 | TTS | g4612.t1 | 3585069 | 3585069 |
Sequences
>g4612.t1 Gene=g4612 Length=1779
ATGAGGAAAAGTTTGCTCTTAATTTGCATAATTTTATGCATTGTTTCATCCACAATAGCT
CAAAAACGTGTTGTTTGTTATTGGGATTCTACATCACTTCGTCATGAAAATTTAAAAGGA
TATTTTTCAGTGGAAGACATCGATCCAACAGTTTGCACTCATATAATCTATACGTACATC
CGATTTTTGCAGAGTAATATCATACTTGAGCAAAATATTAATGACACAATTGTTGCCTTC
ATTAACCTCAAACAGAAAAATCCGAATGTGAAGTTGCTGATTGGTGTTGGCGGACCAAAT
CAACCATCACAATATTTCTCGTTAATTGCAAGTACTGAAACAGACAGAGCCTTGTCAGCA
GCAAAAGTGAAAGAATATATTGATAATTTTGGTTTCGATGGTGTTGATCTTGATTGGCAA
TATCCAAATCAAGGTACAGGTTCAACAGAAGCAGATAAAGAGAACTTTGTTTACCTTCTT
ATGACATGGCGTCAAAAATTGACAAATTCAAAAATTTTTTCAATATCAGTTGGACCGAGA
CAGCGAGACATAAGTTTCTCTTATAATATAGCAGAAATACATCAACATGTCGATTTTATC
AATCTCAATACTTACAATTTACGAGGTTCAGCAAATGATGTTGTCACTGCATTTCATTCG
CCATATCGTAAGAAAAGTGGTGAAAGTGGAGAAGAGTCCGAATGGAATGCTGAGTCAATT
GTCGACAATTGGATTGTACGCGGCTGTCCTGCATCGAAATTGACACTCGGTATTGCAACA
TATGGTCGAAGTTACACATTATCGGATATCAGTCAAACTGGCATTGGTGCGCCTGTTGAT
AGCATTGGAAAAGTAAGCTCTTACTTCCGTACCTTGACAAATAATGCAGGAATTTTGCCA
TATGCTGAAATTTGCTACAATGTAAAGAATAATGGTTGGAAAGAAACACTTGATGAAGAA
CATTTTGCATTTTATGCCGTCTTTAATGATGATCAATGGGTTTCATATGACGCAAAAGAA
TCTGTCATTGCAAAAATTCATTTAGCACGAAATAAAAGACTTGGAGGTGTCAATTATGCT
TCTATCGATCGTGATGATTTTTCTAATGAATGTGGTGAGGGAAGTTTTCCACTTATTCGA
ATTGGTTATGATGGAATTGTTGATGATAATATCGAAGATAATCCAATCGATCTAAAGACA
ACATTAGCAACGACAACTTTATCGACAACTCGTCCAGGAGGAATAACGACAACTCCTCGT
CAAATTACAGTAGAAACGACATCTTCAACAACCACTCGTCCTGGAGGAGTTACAACAACA
CAAAAACCTTCTACAACAACAACACAATCTACTACTACGACTTCAGTATTTTCAACGCCA
CCAACAACCTCAGTTTCACCCACATCAACACGCACCACGACGACCCGAATTACGTCGACA
ACTCGTAAACTTCCTGATGAATTAACACCGCCATTACTCACAGGTCCAGCATCAACAGTG
ACCGTAACAAAAATACCCGATGACGAAGTAAAAACCACAAGCAGTTCCGGTACTAAAAGT
ACAACAGTGAAAAGATCTTCTGTTTCAACTCTCGGAACATTTCCAACGCCACCGACAATG
AGCGGGTTGCAAGGAATTTCGTCCACTACAGTAGATACCGCTGAAATTCCAACAACACGA
ACTTCAACTTTACCGTCAACTACAAAAACAACCACAAAGAAAACAACAACCACACATTTT
CCAACTCCACCTGTCACTTCATTTTCAATTTGGCAATAA
>g4612.t1 Gene=g4612 Length=592
MRKSLLLICIILCIVSSTIAQKRVVCYWDSTSLRHENLKGYFSVEDIDPTVCTHIIYTYI
RFLQSNIILEQNINDTIVAFINLKQKNPNVKLLIGVGGPNQPSQYFSLIASTETDRALSA
AKVKEYIDNFGFDGVDLDWQYPNQGTGSTEADKENFVYLLMTWRQKLTNSKIFSISVGPR
QRDISFSYNIAEIHQHVDFINLNTYNLRGSANDVVTAFHSPYRKKSGESGEESEWNAESI
VDNWIVRGCPASKLTLGIATYGRSYTLSDISQTGIGAPVDSIGKVSSYFRTLTNNAGILP
YAEICYNVKNNGWKETLDEEHFAFYAVFNDDQWVSYDAKESVIAKIHLARNKRLGGVNYA
SIDRDDFSNECGEGSFPLIRIGYDGIVDDNIEDNPIDLKTTLATTTLSTTRPGGITTTPR
QITVETTSSTTTRPGGVTTTQKPSTTTTQSTTTTSVFSTPPTTSVSPTSTRTTTTRITST
TRKLPDELTPPLLTGPASTVTVTKIPDDEVKTTSSSGTKSTTVKRSSVSTLGTFPTPPTM
SGLQGISSTTVDTAEIPTTRTSTLPSTTKTTTKKTTTTHFPTPPVTSFSIWQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g4612.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 24 | 380 | 2.3E-96 |
| 9 | g4612.t1 | Gene3D | G3DSA:3.10.50.10 | - | 260 | 336 | 2.3E-96 |
| 16 | g4612.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 425 | 482 | - |
| 17 | g4612.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 509 | 529 | - |
| 2 | g4612.t1 | PANTHER | PTHR11177 | CHITINASE | 12 | 454 | 1.7E-80 |
| 3 | g4612.t1 | PANTHER | PTHR11177:SF360 | CHITINASE 12-RELATED | 12 | 454 | 1.7E-80 |
| 1 | g4612.t1 | Pfam | PF00704 | Glycosyl hydrolases family 18 | 23 | 359 | 1.2E-66 |
| 11 | g4612.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 12 | g4612.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 13 | g4612.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 14 | g4612.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 20 | - |
| 10 | g4612.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 592 | - |
| 15 | g4612.t1 | SMART | SM00636 | 2g34 | 22 | 365 | 5.0E-69 |
| 5 | g4612.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 22 | 384 | 4.11E-63 |
| 4 | g4612.t1 | SUPERFAMILY | SSF54556 | Chitinase insertion domain | 261 | 336 | 2.26E-14 |
| 7 | g4612.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
| 6 | g4612.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 27 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008061 | chitin binding | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed