Gene loci information

Transcript annotation

  • This transcript has been annotated as DnaJ homolog subfamily A member 2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g4617 g4617.t2 isoform g4617.t2 3636030 3642665
chr_2 g4617 g4617.t2 exon g4617.t2.exon1 3636030 3636136
chr_2 g4617 g4617.t2 exon g4617.t2.exon2 3640585 3642441
chr_2 g4617 g4617.t2 cds g4617.t2.CDS1 3641302 3642441
chr_2 g4617 g4617.t2 exon g4617.t2.exon3 3642594 3642665
chr_2 g4617 g4617.t2 cds g4617.t2.CDS2 3642594 3642665
chr_2 g4617 g4617.t2 TSS g4617.t2 3642963 3642963
chr_2 g4617 g4617.t2 TTS g4617.t2 NA NA

Sequences

>g4617.t2 Gene=g4617 Length=2036
ATGGATGCGGATAACAAACTATATGAGCTTTTGAATGTATCAAGGAATGCAACTGATACT
GAAATTAAGAAGAGCTATCGAAAGCTGGCAATGAAGTATCATCCTGATAAAAATCCAAAT
CCTGAAGAAGCTGAACGATTCAAAGAAATTACATATGCTTACGAAATCCTCTCAGATCCA
GAGAAGAGAAAAACTTACGATCGTTATGGTGTGAAAGGTTTGCAAGAAGGTGGTGCAGAT
AGTAGCGATATGTTTAGTCATATTTTTGGTTCATTCTTTGGCGGAGGAAGTAGTCGGTCA
TCTGGACCTCAACAATGTGAACCAATTGTAATTCAAGAACTTGTAACGTTAGAAGATCTT
TATGTTGGCGGGCGTGAAACTCCTCGCAAGGTAACAAGAATTGTTTGCTGCTCAAAATGT
AATGGTCAAGGTGGCAAGAATGTGAAAAAGTGCAAGGAATGTAGAGGTACAGGCACAAAA
GTTATTATTCATCAAATGGGATTTATGACTCAACAAATTGCAACTAAATGCAATGATTGT
GATGGTAGTGGCGAGTTTATAGACAAAAAAGATCGTTGTGATGCATGCAAAGGATCAAAA
ACGGTTGAAGAGAAAAAAGAGATTACAATTCATATTGATAAGGGCATGAAACATGGACAG
AAAATCGTATTTCGTGGTGAAGGACATCACATGCCAGATTCAGTTCAGGGCGATATTATT
GTTGTGCTCAAAGAGCAAGCACATGAGACATTTAAGCGTGAAGGAAATGACTTAATATTA
AATGAGACAATTTCCATAACGCAGGCTCTCTGTGGCTTCGACTTAGTGATAAAACATTTA
GATGGTCGAGATCTTCATGTTAAACATGAAGCTGGAAATGTTATGAAAAATGGCGATATT
AAGTGCATCGTTGGAGAAGGGATGCCAATTTATAAAAATCCTTTTGAGAAAGGAGTTCTG
TTCATGAATTTCACTGTCGAATTCCCCACGAGCATGGATGTATCAGTTCTTGCTGAGCTC
GAAAAGTGTTTGCCACCGCGACCAGCCTTTGTTATGCCTGACGGTGAACATGTAGAAGAA
GTCAGTTTGTCCGATTATGATCCGCATGCTAGATCAGGAAGAAAGAAGCATTCAGCAGCT
TACAATTCTGATTCTGAAGACGAAGAAAACGGTGGTGCTGGAGGTCCTGGTGTTCAATGT
CGTGCATCGTAAATTATTTAGTAAAACCTTTTTCAAAACACTAAAAACAGTAAAAGTGTT
GTAATCGCTGGTGAATGGAAATTTTTCGTAAGAAAAAGTATATATTCTGATTTAATTCTC
CATATTTTTTTTTTTATTGCAAATCACCTTCAAGTAACTCATGCTCAAGCACCAAAATGC
GTGAATTTGTAGCGACATTAACATTAATGTCTATTTAGTAAATGCGACTTTTTTGCTTTC
ATATTTTTGATAAATCGAACACAAATAGAATGTTTCAATAATCCTCCTCAAATATTCCAT
AATAATTGTAACATTATTTATAATTAAATTTTATTTTTTGATTTGATAGTGGCAACAAAC
ACTATGCATAATAATTTATTTCAACAAAATCTCTTTGCCCAGTTAGTTAAATATCATTAA
TTTGATATTTAGATAATAAATAAAACAAATTAAAAATTTTAAAAAATAATTGAATGAAGA
AATCTGACGATAAACATAAAACATATATGATTGTGATTTAGCATTGAATGAATGATCGTG
AAGGAAACAAACAACCATGATTAAATTATAAATTCAGTATATAACAATTTATTAATTAAT
GTCTTAGATAACGTACATTAAAAAACACAATAATCCTTCATCATCTTTTATGAACAATTC
CTTTTTCAATCAGATTGATTTCTCTTAAAAGAAAGAAGGAGAAATGTACAAGACGAGAAA
AAGAAAAAAGAGAAACACATGGTTACCAAACTTAAGATTATTATGTCAAATTATGACTCA
TTTTTTAGCTAATTTCAAAAATCTTCTATCTCTTACTCTTTACATACGAAATAAAA

>g4617.t2 Gene=g4617 Length=403
MDADNKLYELLNVSRNATDTEIKKSYRKLAMKYHPDKNPNPEEAERFKEITYAYEILSDP
EKRKTYDRYGVKGLQEGGADSSDMFSHIFGSFFGGGSSRSSGPQQCEPIVIQELVTLEDL
YVGGRETPRKVTRIVCCSKCNGQGGKNVKKCKECRGTGTKVIIHQMGFMTQQIATKCNDC
DGSGEFIDKKDRCDACKGSKTVEEKKEITIHIDKGMKHGQKIVFRGEGHHMPDSVQGDII
VVLKEQAHETFKREGNDLILNETISITQALCGFDLVIKHLDGRDLHVKHEAGNVMKNGDI
KCIVGEGMPIYKNPFEKGVLFMNFTVEFPTSMDVSVLAELEKCLPPRPAFVMPDGEHVEE
VSLSDYDPHARSGRKKHSAAYNSDSEDEENGGAGGPGVQCRAS

Protein features from InterProScan

Transcript Database ID Name Start End E.value
18 g4617.t2 CDD cd06257 DnaJ 8 59 2.03114E-24
19 g4617.t2 CDD cd10719 DnaJ_zf 137 200 1.12518E-17
20 g4617.t2 CDD cd10747 DnaJ_C 201 332 1.57816E-41
15 g4617.t2 Gene3D G3DSA:1.10.287.110 - 3 105 2.0E-34
17 g4617.t2 Gene3D G3DSA:2.60.260.20 Urease metallochaperone UreE 112 241 1.0E-37
14 g4617.t2 Gene3D G3DSA:2.10.230.10 - 137 200 1.0E-37
16 g4617.t2 Gene3D G3DSA:2.60.260.20 Urease metallochaperone UreE 248 330 2.5E-24
5 g4617.t2 Hamap MF_01152 Chaperone protein DnaJ [dnaJ]. 3 361 25.712574
23 g4617.t2 MobiDBLite mobidb-lite consensus disorder prediction 361 403 -
24 g4617.t2 MobiDBLite mobidb-lite consensus disorder prediction 361 384 -
4 g4617.t2 PANTHER PTHR43888 DNAJ-LIKE-2, ISOFORM A-RELATED 3 378 1.3E-123
9 g4617.t2 PRINTS PR00625 DnaJ domain signature 8 26 1.1E-28
7 g4617.t2 PRINTS PR00625 DnaJ domain signature 26 41 1.1E-28
6 g4617.t2 PRINTS PR00625 DnaJ domain signature 42 62 1.1E-28
8 g4617.t2 PRINTS PR00625 DnaJ domain signature 62 81 1.1E-28
3 g4617.t2 Pfam PF00226 DnaJ domain 7 67 6.2E-27
1 g4617.t2 Pfam PF01556 DnaJ C terminal domain 115 329 1.7E-34
2 g4617.t2 Pfam PF00684 DnaJ central domain 137 200 2.7E-14
22 g4617.t2 ProSitePatterns PS00636 Nt-dnaJ domain signature. 47 66 -
26 g4617.t2 ProSiteProfiles PS50076 dnaJ domain profile. 6 70 24.054
25 g4617.t2 ProSiteProfiles PS51188 Zinc finger CR-type profile. 124 205 20.53
21 g4617.t2 SMART SM00271 dnaj_3 5 62 3.0E-29
13 g4617.t2 SUPERFAMILY SSF46565 Chaperone J-domain 6 98 2.09E-33
12 g4617.t2 SUPERFAMILY SSF49493 HSP40/DnaJ peptide-binding domain 109 249 8.76E-13
10 g4617.t2 SUPERFAMILY SSF57938 DnaJ/Hsp40 cysteine-rich domain 134 203 2.09E-15
11 g4617.t2 SUPERFAMILY SSF49493 HSP40/DnaJ peptide-binding domain 251 332 2.22E-17

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006457 protein folding BP
GO:0005524 ATP binding MF
GO:0051082 unfolded protein binding MF
GO:0031072 heat shock protein binding MF
GO:0009408 response to heat BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values