General information for g47.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 6a13.
Parent gene
- The parent gene g47 is located on chr_3: 408986..410519 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g47 | g47.t2 | TSS | g47.t2 | 408948 | 408948 |
| chr_3 | g47 | g47.t2 | isoform | g47.t2 | 408986 | 410519 |
| chr_3 | g47 | g47.t2 | exon | g47.t2.exon1 | 408986 | 409335 |
| chr_3 | g47 | g47.t2 | exon | g47.t2.exon2 | 409391 | 410519 |
| chr_3 | g47 | g47.t2 | cds | g47.t2.CDS1 | 409464 | 410519 |
| chr_3 | g47 | g47.t2 | TTS | g47.t2 | 410706 | 410706 |
Sequences
>g47.t2 Gene=g47 Length=1479
ATGGATTTAATTTTAACTGTGTTTTTGCTTACAATACTTAGTCTTTTGTATTTGTGGGTT
AAAAGACGTTTTAGTTATTGGAAAGATCTTGGTTTTTTGCAAGCTGAAGCATCATTTCCT
TTCGGTTCAATAAAAGGAATGGGAATAAAATTATGCACAGCAGAATTCTTGGATATTTAT
TATAAAAAATTTAAAGGAAAAACACCAGCACTTGGATTTTACAATTTTTTTTCACCACAA
ATTTTACCTATTGAGCCTGAATTTATAAAAGATATTTTAGTGAGAGAATTTCAATAATTT
CATGATCGATCATTTTATTACAATAAAAAAGATGATCCATTGAGTGCTAACATTCTCACG
CTTGAAGGTCAAGAATGGCGGGATCGAAGAGTGAAATTATCGCCTATTTTTACATCAGCA
AAAATGAAAATGATGTTTGAAATTATTGATTCAATTGGAGATAAATTTGTAAATTCACTT
GAAAAAGATATGAAACTTTCAAATATTTGTGAAATGCGTCAATGGTGTCAACGATTTACC
AGTGATAACATCGCTAGCACTGCCTTTGGACTGCAATCGAATTGCCTTGAAGATTCAAAT
TCACAATTTATGAAAATGGGCAGAAGACTTTTTGATCTTTCTACTTTTGAAGTTATAAAA
TTTTTTGCTTCTACAGCTTGGCCTGATTTATCTAGAAAACTTGGAATTTTATTTACTCCA
AAAGAAGCAGGTGATTTCTTCCATCATACTTTTCTTCAAACATTTGAAAATCGAACGAAA
AATCCAATTGAACGTCATGATTTTGTTCAATTGCTGTTAAATTTAAAAGACACATTTACA
CCTACTGAATTGGCAGCAGAAGCATTTTTGGTTTATGCAGGTGGTTTTGAATCAAGTTCA
ACATTGATGCAGTTTACACTTTATGAGCTTGCATTGAATCAAGACATGCAAGATCGTTTG
AGAAAAGAATTGATTGAAGGAATTGAAGAAAATGATGGAAAATTGACTTATGATTTGATT
TTTAGCTTCAAATATCTAAATATGGTTATTCAGGAATCTCTAAGAAAATATCCACCAATT
CCAAATACATTCCGAAAAGCCACAAAAGACTATCAATTTCCTGACACAAATTTAATTATC
CCAAAAGGAACGAATATTATTTTAAACACATATTCGTTACATCGTGATCCAGAGTACTTC
CCAGAACCTGAAAAATTTGATCCAGAAAGGTTTAGTGCTGAAAATGTGAGAAATATTCGG
CCGTATACAATGCTTCCTTTCGCAACAGGTCCACGCAATTGTTTGGGTGAAAGATTCGCT
CTTATGCAATCAAAAATTGGAATTACAAAACTAATAACAAATTTTGTTTTCATGCCATGT
GACAAAACAACTATTCCAATGAAATATGTTCCAAGTGCACCGTTTTTGGCACCTGTAAAT
GGAATTCAATTAAAAGTTGAGAAGTTTAAGAAAGATTAA
>g47.t2 Gene=g47 Length=351
MKMMFEIIDSIGDKFVNSLEKDMKLSNICEMRQWCQRFTSDNIASTAFGLQSNCLEDSNS
QFMKMGRRLFDLSTFEVIKFFASTAWPDLSRKLGILFTPKEAGDFFHHTFLQTFENRTKN
PIERHDFVQLLLNLKDTFTPTELAAEAFLVYAGGFESSSTLMQFTLYELALNQDMQDRLR
KELIEGIEENDGKLTYDLIFSFKYLNMVIQESLRKYPPIPNTFRKATKDYQFPDTNLIIP
KGTNIILNTYSLHRDPEYFPEPEKFDPERFSAENVRNIRPYTMLPFATGPRNCLGERFAL
MQSKIGITKLITNFVFMPCDKTTIPMKYVPSAPFLAPVNGIQLKVEKFKKD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g47.t2 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 1 | 351 | 0 |
| 2 | g47.t2 | PANTHER | PTHR24292 | CYTOCHROME P450 | 1 | 347 | 0 |
| 3 | g47.t2 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 1 | 347 | 0 |
| 7 | g47.t2 | PRINTS | PR00463 | E-class P450 group I signature | 142 | 159 | 0 |
| 11 | g47.t2 | PRINTS | PR00385 | P450 superfamily signature | 153 | 170 | 0 |
| 9 | g47.t2 | PRINTS | PR00463 | E-class P450 group I signature | 162 | 188 | 0 |
| 4 | g47.t2 | PRINTS | PR00463 | E-class P450 group I signature | 206 | 224 | 0 |
| 12 | g47.t2 | PRINTS | PR00385 | P450 superfamily signature | 207 | 218 | 0 |
| 6 | g47.t2 | PRINTS | PR00463 | E-class P450 group I signature | 248 | 272 | 0 |
| 5 | g47.t2 | PRINTS | PR00463 | E-class P450 group I signature | 283 | 293 | 0 |
| 13 | g47.t2 | PRINTS | PR00385 | P450 superfamily signature | 284 | 293 | 0 |
| 8 | g47.t2 | PRINTS | PR00463 | E-class P450 group I signature | 293 | 316 | 0 |
| 10 | g47.t2 | PRINTS | PR00385 | P450 superfamily signature | 293 | 304 | 0 |
| 1 | g47.t2 | Pfam | PF00067 | Cytochrome P450 | 4 | 344 | 0 |
| 14 | g47.t2 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 1 | 346 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
