General information for g4730.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein O-mannosyltransferase 1.
Parent gene
- The parent gene g4730 is located on chr_2: 4458663..4461457 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4730 | g4730.t1 | TSS | g4730.t1 | 4455255 | 4455255 |
| chr_2 | g4730 | g4730.t1 | isoform | g4730.t1 | 4455338 | 4458663 |
| chr_2 | g4730 | g4730.t1 | exon | g4730.t1.exon1 | 4455338 | 4455615 |
| chr_2 | g4730 | g4730.t1 | cds | g4730.t1.CDS1 | 4455338 | 4455615 |
| chr_2 | g4730 | g4730.t1 | exon | g4730.t1.exon2 | 4455681 | 4455982 |
| chr_2 | g4730 | g4730.t1 | cds | g4730.t1.CDS2 | 4455681 | 4455982 |
| chr_2 | g4730 | g4730.t1 | exon | g4730.t1.exon3 | 4456383 | 4456802 |
| chr_2 | g4730 | g4730.t1 | cds | g4730.t1.CDS3 | 4456383 | 4456802 |
| chr_2 | g4730 | g4730.t1 | exon | g4730.t1.exon4 | 4457068 | 4457228 |
| chr_2 | g4730 | g4730.t1 | cds | g4730.t1.CDS4 | 4457068 | 4457228 |
| chr_2 | g4730 | g4730.t1 | exon | g4730.t1.exon5 | 4457386 | 4457842 |
| chr_2 | g4730 | g4730.t1 | cds | g4730.t1.CDS5 | 4457386 | 4457842 |
| chr_2 | g4730 | g4730.t1 | exon | g4730.t1.exon6 | 4457969 | 4458663 |
| chr_2 | g4730 | g4730.t1 | cds | g4730.t1.CDS6 | 4457969 | 4458663 |
| chr_2 | g4730 | g4730.t1 | TTS | g4730.t1 | NA | NA |
Sequences
>g4730.t1 Gene=g4730 Length=2313
ATGGGAATTGAAATGAAAGTGTTGGAAAATTGTGAAAATGTTGTAAGAAATCGAAAAACT
CAAACAAAAATTATTGAAAGTATTGAAAATAAACCAAAATGTGAAATTGAAGAAGTAAAA
CCAAAAAAATCTGACACGCAGGAAAAAGTTGAAATTTTAAGTGAAGTTAAAAATGAAAAT
TTCATGATAAAAATTGAATTTGATCCAATGTCAATTTTTTTATTCATTCTTGCCATCGTC
ACAAGATTCTTTCGACTTGCTGAACCAAAGAATGTTGTTTTTGACGAACTTCACTATGGT
AAATTTGTGTCATTATACATGAAAAATGTGTTCTTTTTCGATATGCAGCCACCATTTGGC
AAGCAATTGATTGCAGGAGTTGCTTATTTTGTTGGATATGATGGAAATTACACATTCAAT
AAAATCGGTAATGATTATACCGATAATGTTCCAATATTTTGGCTTCGTGCACTTCCTGCT
TTTTGTGGATGTTTATTGGTTCCAATTATTTACAATTTACTTCTTCAATTGAAAATTAAT
AGATGGATTGCTGCTATAGGTGGATTACTTGTTATTTTTGATAACGCATTACTGACACAA
TCCAGATTCATACTTATGGAGCCAATGTTAATTCTTTTCTCAACCACTGCGCTGCTTTTT
CTCTTGAGATTTCTCGAGAGTCAAAATTTTACAATTAAATGGTGGTTCAATGGAATAACG
TCTGCAATTTTGTATACATTTTCACTAAGTGTGAAATATGTTGGATTTTATTCATTCTTA
TTGGGAATTTTTCTTATTCTTCGGCATTTTTGGAAAATTTTAAGAAATCAAAATTTGACA
AATTTAAAATTATTTTTTGAGACTTTCATCAAAGGATTGATTTTTATTTGTGTTCCAATT
GGAATTTATTTGTCAATTTTTTACGTTCATTTGAATTTACTTTATCGAGCTGGTCCTCAT
GATTCTGTTATGACTTCTGCTTTTCAAGCTTCATTAGATGGTGGACTTGCTTCAATTACT
CATGGACAGCCATTAAAAGTCGTTCACGGGTCACAAATCACTTTGAGGCATACAAACGGT
CGTCCTTGTTGGCTACACTCTCATGCTAACGTTTATCCTGTAAAATATGAAGATAAAAGA
GGTTCATCACATCAACAGCAGGTAACTTGCTACAGTTTCAAAGACGTTAACAATTGGTGG
ATAGTTAAGCGTCCATCCAAACCAGATTTTTCAATTGGTGAAGAACTCGATGTAATAAAA
GACGGTGATGAAATCCAATTAGTGCATGGTTTAACAAGTAGAGCTCTTAATTCACATGAT
GTTGCAGCGCCAATGTCACCACAATCTCAAGAAGTTTCTTGTTACATCGACTACAATATT
TCAATGCCAGGACAATTACTCTGGAGAGTTGAAATTTTAAATAAGGAAGAAGAAGGAAAT
GTTTGGAATGCAATAAGATCTCAAGTAAGACTTGTTCATGTGACAACAAGCACTGCATTG
AGATTCACTGGAAGACAATTGCCACAATGGGGTTATAATCAACATGAAGTTGCAGCTGAT
AAAAATTTAAATCATCCTGATTCAGTTTGGAATGTTGAAGAACATCGATACACTAAAACA
TCTGATCAAAAAGAACGAGAACGACAGCTTTTAAAATCTGAAATGATTCCATTAGAAGCA
ATTGAGCTTTCATTCTGGCAGAAATTTAAAGAACTTCAAATTAAAATGTTTTGGCAATCG
GAAACAATCCAAAATCACATGTATAGCTCAGAACCAATTGAATGGCCGCTGATGTCGAAA
TCGATCGCTTATTGGTATAAAAAAGACACAAATGCACAAATTCATCTTCTTGGAAATTTA
TTACTTTGGTATTCTTGCTCATTTTCAATTTTTATTTATGCTGCAATTTTTGTTTTTTAT
TTATTGAGAAGAAGACGAAATATTTTTGACCTAAATGAAGAAGAATGGACGAAATTTGAA
AATACTGGTTGTGTTTTGTTTATCGGTTATATTTTCAATTTCTTACCATATTTCTTTGTT
GAAAGAACAATGTTTTTGCACAATTACTTGCCTGCTTTAGTTTTTAAAATTTGTCTTTTA
TGTGCAACAATTGAGCATATTTTAAATATTCTGAAAAAATTTAATTTGAGAATTTTAATT
GTTGCTTACAAGCTTTTAGTTTTGTTTTGGCTCTTATACATCTTACATGTTTTCCAAACT
TTCCTAAGTGTCAGTTATGGTCGAACTTCATTGACGTCTGATATGATTATGAAATTAAGA
TGGAAAGACACTTGGGATTTTATATTTTCATAG
>g4730.t1 Gene=g4730 Length=770
MGIEMKVLENCENVVRNRKTQTKIIESIENKPKCEIEEVKPKKSDTQEKVEILSEVKNEN
FMIKIEFDPMSIFLFILAIVTRFFRLAEPKNVVFDELHYGKFVSLYMKNVFFFDMQPPFG
KQLIAGVAYFVGYDGNYTFNKIGNDYTDNVPIFWLRALPAFCGCLLVPIIYNLLLQLKIN
RWIAAIGGLLVIFDNALLTQSRFILMEPMLILFSTTALLFLLRFLESQNFTIKWWFNGIT
SAILYTFSLSVKYVGFYSFLLGIFLILRHFWKILRNQNLTNLKLFFETFIKGLIFICVPI
GIYLSIFYVHLNLLYRAGPHDSVMTSAFQASLDGGLASITHGQPLKVVHGSQITLRHTNG
RPCWLHSHANVYPVKYEDKRGSSHQQQVTCYSFKDVNNWWIVKRPSKPDFSIGEELDVIK
DGDEIQLVHGLTSRALNSHDVAAPMSPQSQEVSCYIDYNISMPGQLLWRVEILNKEEEGN
VWNAIRSQVRLVHVTTSTALRFTGRQLPQWGYNQHEVAADKNLNHPDSVWNVEEHRYTKT
SDQKERERQLLKSEMIPLEAIELSFWQKFKELQIKMFWQSETIQNHMYSSEPIEWPLMSK
SIAYWYKKDTNAQIHLLGNLLLWYSCSFSIFIYAAIFVFYLLRRRRNIFDLNEEEWTKFE
NTGCVLFIGYIFNFLPYFFVERTMFLHNYLPALVFKICLLCATIEHILNILKKFNLRILI
VAYKLLVLFWLLYILHVFQTFLSVSYGRTSLTSDMIMKLRWKDTWDFIFS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g4730.t1 | Gene3D | G3DSA:2.80.10.50 | - | 344 | 545 | 3.1E-59 |
| 4 | g4730.t1 | PANTHER | PTHR10050 | DOLICHYL-PHOSPHATE-MANNOSE–PROTEIN MANNOSYLTRANSFERASE | 41 | 769 | 2.7E-229 |
| 3 | g4730.t1 | Pfam | PF02366 | Dolichyl-phosphate-mannose-protein mannosyltransferase | 73 | 315 | 1.1E-63 |
| 2 | g4730.t1 | Pfam | PF02815 | MIR domain | 365 | 530 | 8.9E-18 |
| 1 | g4730.t1 | Pfam | PF16192 | C-terminal four TMM region of protein-O-mannosyltransferase | 565 | 765 | 4.8E-53 |
| 10 | g4730.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 66 | - |
| 22 | g4730.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 67 | 84 | - |
| 17 | g4730.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 85 | 152 | - |
| 29 | g4730.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 153 | 175 | - |
| 9 | g4730.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 176 | 181 | - |
| 26 | g4730.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 182 | 198 | - |
| 16 | g4730.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 199 | 203 | - |
| 27 | g4730.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 204 | 225 | - |
| 12 | g4730.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 226 | 231 | - |
| 19 | g4730.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 232 | 248 | - |
| 14 | g4730.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 249 | 253 | - |
| 23 | g4730.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 254 | 271 | - |
| 8 | g4730.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 272 | 291 | - |
| 28 | g4730.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 292 | 315 | - |
| 13 | g4730.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 316 | 620 | - |
| 21 | g4730.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 621 | 642 | - |
| 11 | g4730.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 643 | 662 | - |
| 25 | g4730.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 663 | 680 | - |
| 15 | g4730.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 681 | 691 | - |
| 24 | g4730.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 692 | 711 | - |
| 7 | g4730.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 712 | 717 | - |
| 20 | g4730.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 718 | 738 | - |
| 18 | g4730.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 739 | 770 | - |
| 46 | g4730.t1 | ProSiteProfiles | PS50919 | MIR domain profile. | 344 | 405 | 12.004 |
| 44 | g4730.t1 | ProSiteProfiles | PS50919 | MIR domain profile. | 416 | 473 | 10.805 |
| 45 | g4730.t1 | ProSiteProfiles | PS50919 | MIR domain profile. | 479 | 535 | 11.319 |
| 43 | g4730.t1 | SMART | SM00472 | mir_2 | 344 | 405 | 4.5E-15 |
| 41 | g4730.t1 | SMART | SM00472 | mir_2 | 416 | 473 | 1.9E-8 |
| 42 | g4730.t1 | SMART | SM00472 | mir_2 | 479 | 535 | 2.8E-4 |
| 5 | g4730.t1 | SUPERFAMILY | SSF82109 | MIR domain | 342 | 533 | 6.28E-53 |
| 32 | g4730.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 111 | 133 | - |
| 39 | g4730.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 153 | 175 | - |
| 38 | g4730.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 182 | 199 | - |
| 40 | g4730.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 203 | 225 | - |
| 37 | g4730.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 232 | 249 | - |
| 35 | g4730.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 254 | 271 | - |
| 34 | g4730.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 292 | 314 | - |
| 36 | g4730.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 620 | 642 | - |
| 31 | g4730.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 663 | 685 | - |
| 33 | g4730.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 689 | 711 | - |
| 30 | g4730.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 718 | 740 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0006493 | protein O-linked glycosylation | BP |
| GO:0000030 | mannosyltransferase activity | MF |
| GO:0005789 | endoplasmic reticulum membrane | CC |
KEGG
Orthology
- This transcript has been annotated with K00728 (dolichyl-phosphate-mannose-protein mannosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
