General information for g4745.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Glutathione S-transferase 1-1.
Parent gene
- The parent gene g4745 is located on chr_2: 4622082..4622852 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4745 | g4745.t1 | TSS | g4745.t1 | 4622034 | 4622034 |
| chr_2 | g4745 | g4745.t1 | isoform | g4745.t1 | 4622082 | 4622852 |
| chr_2 | g4745 | g4745.t1 | exon | g4745.t1.exon1 | 4622082 | 4622109 |
| chr_2 | g4745 | g4745.t1 | cds | g4745.t1.CDS1 | 4622082 | 4622109 |
| chr_2 | g4745 | g4745.t1 | exon | g4745.t1.exon2 | 4622170 | 4622483 |
| chr_2 | g4745 | g4745.t1 | cds | g4745.t1.CDS2 | 4622170 | 4622483 |
| chr_2 | g4745 | g4745.t1 | exon | g4745.t1.exon3 | 4622538 | 4622852 |
| chr_2 | g4745 | g4745.t1 | cds | g4745.t1.CDS3 | 4622538 | 4622852 |
| chr_2 | g4745 | g4745.t1 | TTS | g4745.t1 | 4622983 | 4622983 |
Sequences
>g4745.t1 Gene=g4745 Length=657
ATGAGTCTAACTTTGTATTATGCGCCTCCGTCGCCTCCATCAAGGACTGTTCTGCTTACT
TTGATGAATTTAGGATTGGATTATGAGTCAAAGCTAATCAATACAATGGCTAGCGAGCAT
AAAACTGAAGAATTTCTTCAACTTAATCCGCAAGGAACAATTCCTGTTGTAGTCCATGAT
GATTTTGTAATGAATGAATCAAGAGCAATTTGTGCTTACTTAGTTAATGCTTTAGCACCA
AATCATTCACTTTATCCTGAAGATCCAAAATTACGGTTCATCATTGATCAACGTATGTTT
TACGATGCTTCTTCATTCACAGCATCCCTCTATACTGCACTCACGCCCATAATGCGCAAA
GGTCAAACAGTTATTCAGCCAGAGCATAAAGACAAAATTATTTCATGCTTGCAAACGCTC
GAGGGATTTTTGGAAGGTTTCGAATGGTTTTCAACAACTGAAAATCCCTGCATTGCAGAT
CTTTCGATTCTAGCATCTTTCTCAACTATTTATCATGCTGGGTTAGATATTGCTGATTTT
CCCAATATTGCTGCATGGTATGAACGTTGTTCGAGTTTAGCCGGCTTCGAAGAAAATGAA
AAAGGAGCAAAAATGTTTGGATTAATGTTGAAGAATAAATTGACAGAGCCATTTTAA
>g4745.t1 Gene=g4745 Length=218
MSLTLYYAPPSPPSRTVLLTLMNLGLDYESKLINTMASEHKTEEFLQLNPQGTIPVVVHD
DFVMNESRAICAYLVNALAPNHSLYPEDPKLRFIIDQRMFYDASSFTASLYTALTPIMRK
GQTVIQPEHKDKIISCLQTLEGFLEGFEWFSTTENPCIADLSILASFSTIYHAGLDIADF
PNIAAWYERCSSLAGFEENEKGAKMFGLMLKNKLTEPF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g4745.t1 | CDD | cd03045 | GST_N_Delta_Epsilon | 3 | 76 | 0.000 |
| 9 | g4745.t1 | CDD | cd03177 | GST_C_Delta_Epsilon | 90 | 207 | 0.000 |
| 6 | g4745.t1 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 1 | 77 | 0.000 |
| 5 | g4745.t1 | Gene3D | G3DSA:1.20.1050.10 | - | 79 | 213 | 0.000 |
| 2 | g4745.t1 | PANTHER | PTHR43969 | GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED | 2 | 212 | 0.000 |
| 1 | g4745.t1 | Pfam | PF02798 | Glutathione S-transferase, N-terminal domain | 1 | 75 | 0.000 |
| 8 | g4745.t1 | ProSiteProfiles | PS50404 | Soluble glutathione S-transferase N-terminal domain profile. | 1 | 82 | 22.188 |
| 7 | g4745.t1 | ProSiteProfiles | PS50405 | Soluble glutathione S-transferase C-terminal domain profile. | 88 | 218 | 14.540 |
| 11 | g4745.t1 | SFLD | SFLDG00358 | Main (cytGST) | 1 | 215 | 0.000 |
| 12 | g4745.t1 | SFLD | SFLDG01153 | Main.4: Theta-like | 1 | 215 | 0.000 |
| 3 | g4745.t1 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 2 | 87 | 0.000 |
| 4 | g4745.t1 | SUPERFAMILY | SSF47616 | GST C-terminal domain-like | 80 | 204 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006749 | glutathione metabolic process | BP |
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed