General information for g4831.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 4d2.
Parent gene
- The parent gene g4831 is located on chr_2: 5164171..5166095 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4831 | g4831.t3 | TSS | g4831.t3 | 5163901 | 5163901 |
| chr_2 | g4831 | g4831.t3 | isoform | g4831.t3 | 5163915 | 5166095 |
| chr_2 | g4831 | g4831.t3 | exon | g4831.t3.exon1 | 5163915 | 5164074 |
| chr_2 | g4831 | g4831.t3 | cds | g4831.t3.CDS1 | 5163915 | 5164074 |
| chr_2 | g4831 | g4831.t3 | exon | g4831.t3.exon2 | 5164169 | 5165334 |
| chr_2 | g4831 | g4831.t3 | cds | g4831.t3.CDS2 | 5164169 | 5165334 |
| chr_2 | g4831 | g4831.t3 | exon | g4831.t3.exon3 | 5165946 | 5166095 |
| chr_2 | g4831 | g4831.t3 | cds | g4831.t3.CDS3 | 5165946 | 5166095 |
| chr_2 | g4831 | g4831.t3 | TTS | g4831.t3 | 5166128 | 5166128 |
Sequences
>g4831.t3 Gene=g4831 Length=1476
ATGTGGTTCATTTACGTTGCTCTGTTTATTTTATTTATTTTTATTCTCGATGATTTTCTA
CTTGATAAGCGGCAATTTAAATTAGCTGAAGTTTTCAATGGACCTAAAAGATTGCCACTT
ATTGGAAATGTTAACATTTTCTTAAACCTTAAACCTAAAGACTATTATGATTTTGTAAAG
GATTTTATGGAAAAATATGCACGCACTGGAACTCGTATGGCACGATATTGGGCTGGAAAT
CAACTTGAGTTGATGGTTGATGACTACAAGTTACTAGAGAAAATTATGTCAAATCCAAAG
TTCATTTCAAAAAGTTCTCAATATGGATTTTTGCATGGAGTGTTGGGTGAAGGACTTTTA
TTTAGCACAGATCAAAAATGGTTCAATCGTCGCAGAATCATCACACCAACTTTTCATTTT
AAAATTCTTGAGCAATTTTTTGAAGTTTTTCGTAAACACAATGAATTGTTAATGGAAGAA
ATCAAAGAAAAATCAAAGAATGGAAAACCAATCAACATTTTTCCAATTGTAACTTCTTCT
GTGCTTAATGCACTTTGTGAAACTGCAATGGGTTATGAAATGAAACCACAAGATTCTGAT
TATTTAAATGCTGTTCGTGAAGTTGGTCATATTGTTGCTACAAGATTCTTAAGTCCTTGG
CTAAGAATTGAATTTATTTACAATATGACAGAAGGAAAAAGAAAGCAAGACAAATGTGCT
AAAATTATGCATGAATTTACAACAAAATTGATTGAAGAACGAAGAAAATTGTTATCTAAA
AGTGAAAAGCAGAATTTATCAAATTTAGATGATGATGATATTGGTTTAAAGAAGAAAATG
TGCTTACTTGATGTTTTACTGCAGTCAACTGTTGACAATAAACCATTAAGTAATGCAGAC
ATTCAAGAAGAAGTTGACACTTTTACTTTTGCCGGACATGATACAACCACAAATTGCATC
TGTTTCACACTTTATTTAATTTCAAAACATCCAGAAGTAAAGGAAAAATTATTGAAAGAA
ATTGAAGAAGTTATTGGTGATGGTGAAGTAACTTTTAAAAGTTTAAATGAATTTAAATAT
CTTGAATTAGTCATTAAAGAATCACTTAGACTTTATCCACCTGCACCAGTGATTTCACGA
AGATTATATGAAGAAGTTGATTTTGGTGATTTTGTCGCTCCAGCTAATGCAAATTACAAT
CTTTGTCTTTATACACTCTTCCGTAATTCTGAAGTTTTTGAGAAGCCAAATGAATTTATT
CCTGAGAGATTTTTGAAAAATATTCCACCATTTGCTTTTGTGCCATTTAGTGCGGTAAGT
TTTCTGGGTCAAAAGTTTGCATTGATAAACATCAAAAACAATTTAATCAACATTTTACGA
AATTTTGAGGTAAATCCAGGAAATGTTGAACCAACATTTGAAATTAATTTGACAATTAAA
TGTGATTCGATGCTTGTCGGTTTTAAACCAAAATAA
>g4831.t3 Gene=g4831 Length=491
MWFIYVALFILFIFILDDFLLDKRQFKLAEVFNGPKRLPLIGNVNIFLNLKPKDYYDFVK
DFMEKYARTGTRMARYWAGNQLELMVDDYKLLEKIMSNPKFISKSSQYGFLHGVLGEGLL
FSTDQKWFNRRRIITPTFHFKILEQFFEVFRKHNELLMEEIKEKSKNGKPINIFPIVTSS
VLNALCETAMGYEMKPQDSDYLNAVREVGHIVATRFLSPWLRIEFIYNMTEGKRKQDKCA
KIMHEFTTKLIEERRKLLSKSEKQNLSNLDDDDIGLKKKMCLLDVLLQSTVDNKPLSNAD
IQEEVDTFTFAGHDTTTNCICFTLYLISKHPEVKEKLLKEIEEVIGDGEVTFKSLNEFKY
LELVIKESLRLYPPAPVISRRLYEEVDFGDFVAPANANYNLCLYTLFRNSEVFEKPNEFI
PERFLKNIPPFAFVPFSAVSFLGQKFALINIKNNLINILRNFEVNPGNVEPTFEINLTIK
CDSMLVGFKPK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g4831.t3 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 31 | 488 | 4.7E-99 |
| 2 | g4831.t3 | PANTHER | PTHR24291:SF143 | CYTOCHROME P450 4D1-RELATED | 8 | 478 | 7.2E-124 |
| 3 | g4831.t3 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 8 | 478 | 7.2E-124 |
| 6 | g4831.t3 | PRINTS | PR00463 | E-class P450 group I signature | 180 | 198 | 2.3E-18 |
| 7 | g4831.t3 | PRINTS | PR00463 | E-class P450 group I signature | 300 | 317 | 2.3E-18 |
| 5 | g4831.t3 | PRINTS | PR00463 | E-class P450 group I signature | 320 | 346 | 2.3E-18 |
| 4 | g4831.t3 | PRINTS | PR00463 | E-class P450 group I signature | 362 | 380 | 2.3E-18 |
| 9 | g4831.t3 | PRINTS | PR00463 | E-class P450 group I signature | 402 | 426 | 2.3E-18 |
| 8 | g4831.t3 | PRINTS | PR00463 | E-class P450 group I signature | 441 | 464 | 2.3E-18 |
| 1 | g4831.t3 | Pfam | PF00067 | Cytochrome P450 | 34 | 473 | 5.6E-86 |
| 14 | g4831.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 15 | g4831.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 16 | g4831.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 16 | - |
| 17 | g4831.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 21 | - |
| 13 | g4831.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 491 | - |
| 11 | g4831.t3 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 34 | 481 | 1.57E-92 |
| 10 | g4831.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 2 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed