General information for g4831.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 4d2.
Parent gene
- The parent gene g4831 is located on chr_2: 5164171..5166095 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4831 | g4831.t4 | TSS | g4831.t4 | 5163901 | 5163901 |
| chr_2 | g4831 | g4831.t4 | isoform | g4831.t4 | 5163915 | 5166095 |
| chr_2 | g4831 | g4831.t4 | exon | g4831.t4.exon1 | 5163915 | 5165334 |
| chr_2 | g4831 | g4831.t4 | cds | g4831.t4.CDS1 | 5164195 | 5165334 |
| chr_2 | g4831 | g4831.t4 | exon | g4831.t4.exon2 | 5165946 | 5166095 |
| chr_2 | g4831 | g4831.t4 | cds | g4831.t4.CDS2 | 5165946 | 5166095 |
| chr_2 | g4831 | g4831.t4 | TTS | g4831.t4 | 5166128 | 5166128 |
Sequences
>g4831.t4 Gene=g4831 Length=1570
ATGTGGTTCATTTACGTTGCTCTGTTTATTTTATTTATTTTTATTCTCGATGATTTTCTA
CTTGATAAGCGGCAATTTAAATTAGCTGAAGTTTTCAATGGACCTAAAAGATTGCCACTT
ATTGGAAATGTTAACATTTTCTTAAACCTTAAACCTAAAGGTGAGTAAAAATCAAAGTCC
AATTCAAACTTTCTTTCAAAATCTATATCTTATCATATTTTGCACGGTCTCGTGTGGCTT
TTATCATATTTTAGACTATTATGATTTTGTAAAGGATTTTATGGAAAAATATGCACGCAC
TGGAACTCGTATGGCACGATATTGGGCTGGAAATCAACTTGAGTTGATGGTTGATGACTA
CAAGTTACTAGAGAAAATTATGTCAAATCCAAAGTTCATTTCAAAAAGTTCTCAATATGG
ATTTTTGCATGGAGTGTTGGGTGAAGGACTTTTATTTAGCACAGATCAAAAATGGTTCAA
TCGTCGCAGAATCATCACACCAACTTTTCATTTTAAAATTCTTGAGCAATTTTTTGAAGT
TTTTCGTAAACACAATGAATTGTTAATGGAAGAAATCAAAGAAAAATCAAAGAATGGAAA
ACCAATCAACATTTTTCCAATTGTAACTTCTTCTGTGCTTAATGCACTTTGTGAAACTGC
AATGGGTTATGAAATGAAACCACAAGATTCTGATTATTTAAATGCTGTTCGTGAAGTTGG
TCATATTGTTGCTACAAGATTCTTAAGTCCTTGGCTAAGAATTGAATTTATTTACAATAT
GACAGAAGGAAAAAGAAAGCAAGACAAATGTGCTAAAATTATGCATGAATTTACAACAAA
ATTGATTGAAGAACGAAGAAAATTGTTATCTAAAAGTGAAAAGCAGAATTTATCAAATTT
AGATGATGATGATATTGGTTTAAAGAAGAAAATGTGCTTACTTGATGTTTTACTGCAGTC
AACTGTTGACAATAAACCATTAAGTAATGCAGACATTCAAGAAGAAGTTGACACTTTTAC
TTTTGCCGGACATGATACAACCACAAATTGCATCTGTTTCACACTTTATTTAATTTCAAA
ACATCCAGAAGTAAAGGAAAAATTATTGAAAGAAATTGAAGAAGTTATTGGTGATGGTGA
AGTAACTTTTAAAAGTTTAAATGAATTTAAATATCTTGAATTAGTCATTAAAGAATCACT
TAGACTTTATCCACCTGCACCAGTGATTTCACGAAGATTATATGAAGAAGTTGATTTTGG
TGATTTTGTCGCTCCAGCTAATGCAAATTACAATCTTTGTCTTTATACACTCTTCCGTAA
TTCTGAAGTTTTTGAGAAGCCAAATGAATTTATTCCTGAGAGATTTTTGAAAAATATTCC
ACCATTTGCTTTTGTGCCATTTAGTGCGGTAAGTTTTCTGGGTCAAAAGTTTGCATTGAT
AAACATCAAAAACAATTTAATCAACATTTTACGAAATTTTGAGGTAAATCCAGGAAATGT
TGAACCAACATTTGAAATTAATTTGACAATTAAATGTGATTCGATGCTTGTCGGTTTTAA
ACCAAAATAA
>g4831.t4 Gene=g4831 Length=429
MEKYARTGTRMARYWAGNQLELMVDDYKLLEKIMSNPKFISKSSQYGFLHGVLGEGLLFS
TDQKWFNRRRIITPTFHFKILEQFFEVFRKHNELLMEEIKEKSKNGKPINIFPIVTSSVL
NALCETAMGYEMKPQDSDYLNAVREVGHIVATRFLSPWLRIEFIYNMTEGKRKQDKCAKI
MHEFTTKLIEERRKLLSKSEKQNLSNLDDDDIGLKKKMCLLDVLLQSTVDNKPLSNADIQ
EEVDTFTFAGHDTTTNCICFTLYLISKHPEVKEKLLKEIEEVIGDGEVTFKSLNEFKYLE
LVIKESLRLYPPAPVISRRLYEEVDFGDFVAPANANYNLCLYTLFRNSEVFEKPNEFIPE
RFLKNIPPFAFVPFSAVSFLGQKFALINIKNNLINILRNFEVNPGNVEPTFEINLTIKCD
SMLVGFKPK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g4831.t4 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 1 | 426 | 0 |
| 2 | g4831.t4 | PANTHER | PTHR24291:SF143 | CYTOCHROME P450 4D1-RELATED | 10 | 416 | 0 |
| 3 | g4831.t4 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 10 | 416 | 0 |
| 9 | g4831.t4 | PRINTS | PR00463 | E-class P450 group I signature | 118 | 136 | 0 |
| 8 | g4831.t4 | PRINTS | PR00463 | E-class P450 group I signature | 238 | 255 | 0 |
| 5 | g4831.t4 | PRINTS | PR00463 | E-class P450 group I signature | 258 | 284 | 0 |
| 4 | g4831.t4 | PRINTS | PR00463 | E-class P450 group I signature | 300 | 318 | 0 |
| 7 | g4831.t4 | PRINTS | PR00463 | E-class P450 group I signature | 340 | 364 | 0 |
| 6 | g4831.t4 | PRINTS | PR00463 | E-class P450 group I signature | 379 | 402 | 0 |
| 1 | g4831.t4 | Pfam | PF00067 | Cytochrome P450 | 2 | 411 | 0 |
| 10 | g4831.t4 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 5 | 419 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed