General information for g4841.t20
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine–tRNA ligase, cytoplasmic.
Parent gene
- The parent gene g4841 is located on chr_2: 5203985..5205147 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4841 | g4841.t20 | TSS | g4841.t20 | 5203197 | 5203197 |
| chr_2 | g4841 | g4841.t20 | isoform | g4841.t20 | 5203286 | 5204222 |
| chr_2 | g4841 | g4841.t20 | exon | g4841.t20.exon1 | 5203286 | 5203377 |
| chr_2 | g4841 | g4841.t20 | exon | g4841.t20.exon2 | 5203466 | 5204222 |
| chr_2 | g4841 | g4841.t20 | cds | g4841.t20.CDS1 | 5203549 | 5204220 |
| chr_2 | g4841 | g4841.t20 | TTS | g4841.t20 | 5205040 | 5205040 |
Sequences
>g4841.t20 Gene=g4841 Length=849
ATGGTTTTGGATATTGAGTTCTTCCGTGGTGATAAGGGAAATGACGTGAATAAAGTCCGA
ACTAATCAGGAAAAGAGATTTAAAGATCTGAAATGCGGAATGGAGACAACGAAGATTTAA
AGGCGATCTCTTCAATAAATTGAAAAATTTATGTAGCAAAGAAATTGGAGAACGTATGAA
AAAGAAAGAAGCCGCTGGTAATGAAGATGATCCAGTTTCAGACGAATTAGTAGCAAAACT
TGAAGAACTCAAGATTGATGACTTAAAACCCTTAACAATCGCTCAAATTAAAAAAGTACG
TACTTTTATCGATGAAGCAATTGTCGAAAATGAGAAGAAAATACTCGAGACTGAGTCGAC
TCGAAATAATGCTTTAAGAGAAGTCGGTAATTGGCTCCACGAGTCTGTTCCTGTTAGCAA
TGACGAAGATGAGAACGGAATTGAAAGAACATGGGGTGATTGTGAAATAAAGAAGAAATA
TTCTCATGTTGATTTAATTCATATGATTGATGGAATGAATGGTGAGAAGGGAGCAGTAGT
TAGTGGAAATCGTGGATATTTTTTAACCGGAGCAGCCGTTTTTCTCGAGCAAGCACTTAT
TCAGCATGCACTTCATATTCTTTTCAACAAAGGCTATACACCACTTTACACTCCATTCTT
TATGCGAAAGGATGTTATGCAGGAAGTAGCACAATTGTCTCAATTTGATGAAGAATTATA
CAAGGTTGTTGGAAAAAATCAAGACAAAGCTGATGAAAATGATGAGAAATATTTAATTGC
AACTTCAGAACAGCCAATTGCAGCTTATCATCGTGATGAATGGATTCCAGAAGCTTCATT
GCCCATAAA
>g4841.t20 Gene=g4841 Length=224
MKKKEAAGNEDDPVSDELVAKLEELKIDDLKPLTIAQIKKVRTFIDEAIVENEKKILETE
STRNNALREVGNWLHESVPVSNDEDENGIERTWGDCEIKKKYSHVDLIHMIDGMNGEKGA
VVSGNRGYFLTGAAVFLEQALIQHALHILFNKGYTPLYTPFFMRKDVMQEVAQLSQFDEE
LYKVVGKNQDKADENDEKYLIATSEQPIAAYHRDEWIPEASLPI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g4841.t20 | Gene3D | G3DSA:1.10.287.40 | - | 1 | 71 | 0 |
| 3 | g4841.t20 | Gene3D | G3DSA:3.30.930.10 | Bira Bifunctional Protein; Domain 2 | 72 | 224 | 0 |
| 1 | g4841.t20 | PANTHER | PTHR11778 | SERYL-TRNA SYNTHETASE | 13 | 224 | 0 |
| 2 | g4841.t20 | SUPERFAMILY | SSF55681 | Class II aaRS and biotin synthetases | 100 | 224 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004828 | serine-tRNA ligase activity | MF |
| GO:0000166 | nucleotide binding | MF |
| GO:0006434 | seryl-tRNA aminoacylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
