General information for g4841.t35
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine–tRNA ligase, cytoplasmic.
Parent gene
- The parent gene g4841 is located on chr_2: 5203985..5205147 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4841 | g4841.t35 | TSS | g4841.t35 | 5203877 | 5203877 |
| chr_2 | g4841 | g4841.t35 | isoform | g4841.t35 | 5203901 | 5204882 |
| chr_2 | g4841 | g4841.t35 | exon | g4841.t35.exon1 | 5203901 | 5204125 |
| chr_2 | g4841 | g4841.t35 | cds | g4841.t35.CDS1 | 5204035 | 5204125 |
| chr_2 | g4841 | g4841.t35 | exon | g4841.t35.exon2 | 5204177 | 5204697 |
| chr_2 | g4841 | g4841.t35 | cds | g4841.t35.CDS2 | 5204177 | 5204697 |
| chr_2 | g4841 | g4841.t35 | exon | g4841.t35.exon3 | 5204757 | 5204882 |
| chr_2 | g4841 | g4841.t35 | cds | g4841.t35.CDS3 | 5204757 | 5204882 |
| chr_2 | g4841 | g4841.t35 | TTS | g4841.t35 | 5205040 | 5205040 |
Sequences
>g4841.t35 Gene=g4841 Length=872
AGGGAGCAGTAGTTAGTGGAAATCGTGGATATTTTTTAACCGGAGCAGCCGTTTTTCTCG
AGCAAGCACTTATTCAGCATGCACTTCATATTCTTTTCAACAAAGGCTATACACCACTTT
ACACTCCATTCTTTATGCGAAAGGATGTTATGCAGGAAGTAGCACAATTGTCTCAATTTG
ATGAAGAATTATACAAGGTTGTTGGAAAAAATCAAGACAAAGCTGCTTATCATCGTGATG
AATGGATTCCAGAAGCTTCATTGCCCATAAAATATGCTGGCTTATCAACTTGTTTTCGTC
AAGAAGTTGGAAGTCACGGTCGCGATACACGTGGCATCTTTCGTGTACATCAGTTTGAAA
AGATTGAACAATTTGTGCTGACATCACCTCACGACAACAAATCATGGGAAATGATGGATG
AAATGATTGGAAACGCTGAGGAGTTTTGTCAATCATTAGGAATTCCTTATCGCATTGTCA
ACATTGTTTCAGGCGCACTTAATCATGCAGCGTCAAAGAAACTTGATTTAGAAGCTTGGT
TCTGTGGATCAGGCGCTTTTAGAGAACTCGTTTCATGCAGCAATTGTCTCGATTATCAAG
CTCGTCGTCTTCTCGTTCGTTATGGAATGACAAAGAAGATGAATGCAAATATTGATTATG
TGCATATGCTAAATGCAACTATGTGTGCAACTACAAGAGTTATTTGCGCAATTCTTGAAA
CTCATCAAACTGAGACTGGTATTAAGGTTCCAGAAGTATTGCAGAAATACATGCCTGAAA
AATATAAACTTGAAATTCCATTTGTTAAACCAGCTCCAATTGAAGAAGAAGAAAAGAAAA
AGGCAAAGAAGGGCAAAAAAGAAGCAGCATAA
>g4841.t35 Gene=g4841 Length=245
MRKDVMQEVAQLSQFDEELYKVVGKNQDKAAYHRDEWIPEASLPIKYAGLSTCFRQEVGS
HGRDTRGIFRVHQFEKIEQFVLTSPHDNKSWEMMDEMIGNAEEFCQSLGIPYRIVNIVSG
ALNHAASKKLDLEAWFCGSGAFRELVSCSNCLDYQARRLLVRYGMTKKMNANIDYVHMLN
ATMCATTRVICAILETHQTETGIKVPEVLQKYMPEKYKLEIPFVKPAPIEEEEKKKAKKG
KKEAA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g4841.t35 | Gene3D | G3DSA:3.30.930.10 | Bira Bifunctional Protein; Domain 2 | 1 | 229 | 0.000 |
| 2 | g4841.t35 | PANTHER | PTHR11778 | SERYL-TRNA SYNTHETASE | 1 | 224 | 0.000 |
| 5 | g4841.t35 | PRINTS | PR00981 | Seryl-tRNA synthetase signature | 57 | 69 | 0.000 |
| 7 | g4841.t35 | PRINTS | PR00981 | Seryl-tRNA synthetase signature | 69 | 82 | 0.000 |
| 6 | g4841.t35 | PRINTS | PR00981 | Seryl-tRNA synthetase signature | 111 | 124 | 0.000 |
| 4 | g4841.t35 | PRINTS | PR00981 | Seryl-tRNA synthetase signature | 128 | 144 | 0.000 |
| 3 | g4841.t35 | PRINTS | PR00981 | Seryl-tRNA synthetase signature | 146 | 162 | 0.000 |
| 1 | g4841.t35 | Pfam | PF00587 | tRNA synthetase class II core domain (G, H, P, S and T) | 31 | 196 | 0.000 |
| 10 | g4841.t35 | ProSiteProfiles | PS50862 | Aminoacyl-transfer RNA synthetases class-II family profile. | 42 | 206 | 14.471 |
| 8 | g4841.t35 | SUPERFAMILY | SSF55681 | Class II aaRS and biotin synthetases | 1 | 214 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004828 | serine-tRNA ligase activity | MF |
| GO:0006418 | tRNA aminoacylation for protein translation | BP |
| GO:0000166 | nucleotide binding | MF |
| GO:0004812 | aminoacyl-tRNA ligase activity | MF |
| GO:0006434 | seryl-tRNA aminoacylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
