General information for g4889.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Alpha-N-acetylglucosaminidase.
Parent gene
- The parent gene g4889 is located on chr_2: 5823212..5825202 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4889 | g4889.t1 | TSS | g4889.t1 | 5820685 | 5820685 |
| chr_2 | g4889 | g4889.t1 | isoform | g4889.t1 | 5820775 | 5823212 |
| chr_2 | g4889 | g4889.t1 | exon | g4889.t1.exon1 | 5820775 | 5821002 |
| chr_2 | g4889 | g4889.t1 | cds | g4889.t1.CDS1 | 5820775 | 5821002 |
| chr_2 | g4889 | g4889.t1 | exon | g4889.t1.exon2 | 5821116 | 5823090 |
| chr_2 | g4889 | g4889.t1 | cds | g4889.t1.CDS2 | 5821116 | 5823090 |
| chr_2 | g4889 | g4889.t1 | exon | g4889.t1.exon3 | 5823151 | 5823212 |
| chr_2 | g4889 | g4889.t1 | cds | g4889.t1.CDS3 | 5823151 | 5823212 |
| chr_2 | g4889 | g4889.t1 | TTS | g4889.t1 | 5823348 | 5823348 |
Sequences
>g4889.t1 Gene=g4889 Length=2265
ATGAAGCATACTATTTTCATCATAGTATCTCTACTATTCTTCACAATTAATTCAATAACT
TGTAAGTTGGATAATGAGGACGATGAGTTAGCAAGAATAATAAGACCAATAACAGATGAA
AAAACACAACAGCAGGCAGCGTTAAATGTAATTAGACGCTTAATTCATGAGCGTGCTGAT
AATGTAGCGATAAAAGTAAACTTTAAATTGCCTGTCAATTATTTTAAGATTCGTAAAACA
AACAACTCGGAAATGTTGAGAATTGAGGCGAGTAGTGGAGTTGCCGCAACTAAAGCTTTT
CATTACTATCTCAAACATTTTTGTGGTGTTCATATCTCATGGGATGGCCATCAGTTAAAA
ACATTAGAGCAACTCGATGAATTTCCTGCAGTTAATATTGAAGTTGAGGCATCAAGTAAA
ATTGTTTATTATCAAAATGTTTGCACTCATAGTTATAGTTATTCTTTTTGGAAATTCGAA
CAATGGCGAGAACACATTGATTTTATTGCACTATCAGGAATTACATTGACATTGGCTCCG
TTTCAAGAGGACGTTTGGACAGAAATTTATCGTGAATATGGAATGACTCAGGATGAAATT
GATGAACATTTAGCTGGTGTTGGTTTCTTTGCATGGCAACGAATGGGAAATATACGTGGA
TGGGGAGGAAAATTATCAAAAAATTTCATAGAATTTGCATCTAATTTGCAACAACAAATA
ATTACTGCACTTAATGAGCTTGGAATTTCTGTTGCGATTCAAGCTTTTGATGGACATTTG
CCAATTCATTTTAAAACAATTTTCCCTAACGCCTCATTTTCATCTACTACACAATGGAAC
GATTTTCCGCCGCAATATTGCTGTCCATTATTTATTGAACCAATTGATCCGCTTTTTAGA
GAGATTGGTGAAAAGTTTTTACGTGCAATGATTCAAAGGTATGGCACAAATCACATTTAT
TTTAGTGATCCATTCAACGAAGTTCAACCGCGAATTTCTGATGCTAATTATCTTCGCAAT
GTTTCGCTTTCAATCTATTCTGGTATGCAAGCTGTCGATTCGAATGCAATTTGGTTGCTT
CAGGGATGGTTTCTTGTTAATAATCCAGACTTTTGGAATGATGAAATCACAAAAGCTTTT
CTAACTGCTGTTCCACATAATAAACTTCTCATACTGGACCTTCAAAGTGAACAACATCCT
CAATATAATCGTACAAGTTCATTTTATGGTCAGAAATTTATTTGGTGTATGCTTCACAAT
TTCGGTGGAACTTTAGGCATGCATGGTTCAATTCGTCTAATGAATGTAGAAATTCCACGT
GCTGCTAATATGGAAAATTCATCAATGATTGGCGTTGGAATCACACCGGAAGGAATTCAT
CAAAATTACGTCGTTTATGAGTTTGCATTAGAAAAAGCGTGGGATTATCAAGAGATTAAT
CATAAGAAATGGATAAAAAATTATGCTCGAAATCGTTATGGACTTGTTTCTCATGAAACG
ACATCAATAGAGCACGCTTGGCTGCTTCTTCTGAAAAGTGTTTATGCATTCGAAGGTCTT
CAAAACATTCGAGGCAAATACGTCTATTGTAGACGTCCTTCTTTACGTATTCAAGTATGG
AGATGGTATGATGAAAAATTGGTAATAAAAGCACTTGGAAAATTTTTGGCTATAAATGAA
AGCGATTTGTTAAATGACCATTTATATGAACGCGATTTAGTCGATTTGACGCGCCAATTG
CTGCAAATTCAGGCAGAATTTATCTATACGACTCTCGTCGAGACTTTCAAAAAGAAAGAT
TTGAGAAAATTTGCAATTGCTTCTGAAATGTTTTTGGAATTATTGAGGGATTTAGATAGT
CTACTTGCGACACATGATGATTTTCTTTTGGGTAAATTTCTCGAGGATGCAAAAGCAATG
GCATTGGATGATGACGAACGGAAACAATTGGAATTTAATGCTCGAAATCAAATCACTTTA
TGGGGACCTGACGGGCAAATTCTTGATTATGCTACAAAACAATGGAATGGAATCGTGATG
GATTACTATCTTCCAAGATGGACACTCTTTATACAGCAGCTCAACATTTCATTACAGACA
AATATTGCATTCAATCAATCAAAATTCCAACAAGATGTATTTAATCAAGTTGAATATCCC
TTTAATGAAAATAACGCAAAGTTTTATCCAAGCAAAGCAAATGGAAATGTAGTCAACAAA
GCAAAAGAACTGTTTCACAAATGGAAAAGAATTACTTTTAATTAA
>g4889.t1 Gene=g4889 Length=754
MKHTIFIIVSLLFFTINSITCKLDNEDDELARIIRPITDEKTQQQAALNVIRRLIHERAD
NVAIKVNFKLPVNYFKIRKTNNSEMLRIEASSGVAATKAFHYYLKHFCGVHISWDGHQLK
TLEQLDEFPAVNIEVEASSKIVYYQNVCTHSYSYSFWKFEQWREHIDFIALSGITLTLAP
FQEDVWTEIYREYGMTQDEIDEHLAGVGFFAWQRMGNIRGWGGKLSKNFIEFASNLQQQI
ITALNELGISVAIQAFDGHLPIHFKTIFPNASFSSTTQWNDFPPQYCCPLFIEPIDPLFR
EIGEKFLRAMIQRYGTNHIYFSDPFNEVQPRISDANYLRNVSLSIYSGMQAVDSNAIWLL
QGWFLVNNPDFWNDEITKAFLTAVPHNKLLILDLQSEQHPQYNRTSSFYGQKFIWCMLHN
FGGTLGMHGSIRLMNVEIPRAANMENSSMIGVGITPEGIHQNYVVYEFALEKAWDYQEIN
HKKWIKNYARNRYGLVSHETTSIEHAWLLLLKSVYAFEGLQNIRGKYVYCRRPSLRIQVW
RWYDEKLVIKALGKFLAINESDLLNDHLYERDLVDLTRQLLQIQAEFIYTTLVETFKKKD
LRKFAIASEMFLELLRDLDSLLATHDDFLLGKFLEDAKAMALDDDERKQLEFNARNQITL
WGPDGQILDYATKQWNGIVMDYYLPRWTLFIQQLNISLQTNIAFNQSKFQQDVFNQVEYP
FNENNAKFYPSKANGNVVNKAKELFHKWKRITFN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g4889.t1 | Gene3D | G3DSA:3.30.379.10 | Chitobiase | 19 | 136 | 2.3E-14 |
| 10 | g4889.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 139 | 476 | 6.5E-112 |
| 9 | g4889.t1 | Gene3D | G3DSA:1.20.120.670 | - | 478 | 753 | 1.8E-72 |
| 4 | g4889.t1 | PANTHER | PTHR12872 | ALPHA-N-ACETYLGLUCOSAMINIDASE | 11 | 748 | 1.9E-239 |
| 5 | g4889.t1 | PANTHER | PTHR12872:SF1 | ALPHA-N-ACETYLGLUCOSAMINIDASE | 11 | 748 | 1.9E-239 |
| 1 | g4889.t1 | Pfam | PF12971 | Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain | 46 | 124 | 6.5E-17 |
| 2 | g4889.t1 | Pfam | PF05089 | Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain | 143 | 475 | 6.7E-122 |
| 3 | g4889.t1 | Pfam | PF12972 | Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain | 484 | 747 | 5.9E-73 |
| 12 | g4889.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 13 | g4889.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 14 | g4889.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 14 | - |
| 15 | g4889.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 18 | - |
| 11 | g4889.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 754 | - |
| 6 | g4889.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 151 | 373 | 8.43E-6 |
| 7 | g4889.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
- This transcript has been annotated with K01205 (alpha-N-acetylglucosaminidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
