General information for g4919.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Tyrosine-protein kinase hopscotch.
Parent gene
- The parent gene g4919 is located on chr_2: 5960936..5962852 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4919 | g4919.t1 | isoform | g4919.t1 | 5958110 | 5962852 |
| chr_2 | g4919 | g4919.t1 | exon | g4919.t1.exon1 | 5958110 | 5958358 |
| chr_2 | g4919 | g4919.t1 | cds | g4919.t1.CDS1 | 5958110 | 5958358 |
| chr_2 | g4919 | g4919.t1 | exon | g4919.t1.exon2 | 5958458 | 5958810 |
| chr_2 | g4919 | g4919.t1 | cds | g4919.t1.CDS2 | 5958458 | 5958810 |
| chr_2 | g4919 | g4919.t1 | exon | g4919.t1.exon3 | 5958897 | 5959089 |
| chr_2 | g4919 | g4919.t1 | cds | g4919.t1.CDS3 | 5958897 | 5959089 |
| chr_2 | g4919 | g4919.t1 | exon | g4919.t1.exon4 | 5959362 | 5960384 |
| chr_2 | g4919 | g4919.t1 | cds | g4919.t1.CDS4 | 5959362 | 5960384 |
| chr_2 | g4919 | g4919.t1 | exon | g4919.t1.exon5 | 5960448 | 5960877 |
| chr_2 | g4919 | g4919.t1 | cds | g4919.t1.CDS5 | 5960448 | 5960877 |
| chr_2 | g4919 | g4919.t1 | exon | g4919.t1.exon6 | 5960936 | 5961321 |
| chr_2 | g4919 | g4919.t1 | cds | g4919.t1.CDS6 | 5960936 | 5961321 |
| chr_2 | g4919 | g4919.t1 | exon | g4919.t1.exon7 | 5961385 | 5961702 |
| chr_2 | g4919 | g4919.t1 | cds | g4919.t1.CDS7 | 5961385 | 5961702 |
| chr_2 | g4919 | g4919.t1 | exon | g4919.t1.exon8 | 5962343 | 5962852 |
| chr_2 | g4919 | g4919.t1 | cds | g4919.t1.CDS8 | 5962343 | 5962852 |
| chr_2 | g4919 | g4919.t1 | TSS | g4919.t1 | NA | NA |
| chr_2 | g4919 | g4919.t1 | TTS | g4919.t1 | NA | NA |
Sequences
>g4919.t1 Gene=g4919 Length=3462
ATGTGGGCAATGGAAACTCGAGAAATCAAAAATTATATATTGAATTACAAAACTGGCAAA
TTTAAAGAGATAAAGCATGACAAGGACGAGACATGTGAAGATTTTTGTAAAGAGTTATGC
CGCAGATGGAATTTTCCACCTTTAGTGCAATTGTTGTTTGGCCTTCGTCTACATGGTACT
AAAATATGGCTTGGAAGTGCACGTCAACTTGTTGCTGATAAACATTATGAATTCAGAATT
CGAATCAAAATTCCTAAATTAGCTGATCTAAACAAGCATGATAAGAACACGTTTGACTAC
TTTTATCATCAAGTACGCTACGATGTACTCCATAATGATATACCTGGACTTATCAATGAG
AATACAAGAAACAAAATTCTTGGTCTTTGTGTCACAGACATGTATGTTGAGATGTTAGAA
AATGAAAATAAAGACTATCCGAATGAGACAAGCAGAAAAAAGGCACGTGAGGAGAAAATG
AAATATTTGAATGTAAACTACAAGAATTACATTCCAAGATTTTTATATGAAAAACATTGG
GTAATATTGCAAATGCGGATCAAAAAATCACTGAAAGGCGTTGATTATAAACATGATCCT
CTTTATGTTAAAACGTCATATATACAACAAGTCGATTCAATGGCACCAAACTATTTGATA
GAAGAATATTGGGGTAAAATTGCATACCCGCAAGAAGATCATATGAGACAAGGAACATGT
CGTGTAAGACTTCAAATTGCACCTTACGACAAAGAACAACCTGGATTGAGAATGCATTAT
GCATATAAAGATACTTGGCGACATATTTCAACATTTGCTGATTTTTATGCTATTCAAATT
GATGCAGATGCTAAGCAAGTGAGATTAGAAATTCAAAACTCACCTCAAGGTTTCCCTATA
ACGATGGATAGTATTGAAGAAATTGAATCATTTGTGAATTGTATGAATATCTATTATCGA
TTGACAGTAAAGTGGACTTGGTATCTCTGTGAGTTGCTTAAATCGCCATCACTTGATTTT
CTTAACAAATATAAAATTCACGGCCCAATTGGTGGAGGATATTCTTATAATAGAATAAAA
GAAATTGGAAAGGGAGTTGGCACATATATCATTCGACAATGTGAAAAAGAATTTGACATA
TATTACATTGATATTTTAACCAAAAAAAATCAAAGTTGTGAAACATTTAAAATAAATGGT
GCTGCAGAAAAATGGCAACTTTATGATAATGAAAATAACGAAATTTCAGCAGAATTTGAT
AATCTTGTGTCACTTGCAAAAAGCATACCCGTTGAAAGTGGTGTCTATAATCGATTACCA
CCGTCATGTTATGAAAAGCCACCTCTATTGCTTCTATGCCAAACTAATATTAAATCTGTA
CCTGCTTCAGCGACGAATACAACGATGATCACAACAACTCAAAATTCACCAGGTGGTTTG
AGAACACAACGACCAGTCGTATTCACTAACGAAGATTTTAGAATGTATATTCCGAGTACA
CGTGAAATTAATGATGAAGCATTTGAGCAGAGAAAAGCTGAATATCGTGATAGAAATACT
GAAGTTACATTGAAACTTTTAAAGACTACCGAGAAATTGACTGAATTTCATTTATTAGCT
GACAAGTGGTCAAAATTAGATATTTCTGAAATAGTAAAATTGAATGGAATTATTCTCAAT
CCAGTTGCTCTTGTTCTTGAACCATTAAAATATGGACCTTTAGATATATTTTTAAGAACA
CACGAATTTAGAAGACAAGTTGTTCCACTAAATCTTGTCGAGACTGCATATTCATTAGCA
AGAGCACTCCACTACTTGCAAGAAAAGCAAATCGTTCACGGTAGAATAAAATGTTCCAGT
TTAGAAGTGATTAAATTTGATCCAGGAAATTCATTTGAAGTAAAACTCGGTGATCCCGGT
TTACCGCGTGATTTGAAAGTTCGTGATGTGCCATGGATTCCGATTGAAGATTATGATGAT
CTCAATAATAGCAGAAACAATTTGAAAGCTGACATATGGGCATATGCCACAACATTATGG
GAGATATTTTCTAGAGGAGAATCTCCATTCACAGAACTTTCAAAAGTTCCAAATATTACT
GAATTTTTCCGAAGAGGAGATCGACTACCAAAGCCAAAAGAATGTGAATTACTGCCAAGA
ATTTATGAGATAATGAAATCAGGATGGGAAGTTGAACCTGAAAAAAGATTTGCACCACAA
ACAATTTTTTCACCATTGCTTGATATAAGTAGGAATTTGTCACGACATTATGAAAGTCCT
ATAAGTTCAAATCCTCGAAGTAATGGAACAATGCAAAGAATGAATGGAGGCATTCGCAAT
GGTCGTCCAAGTTCTTCAAATTCAATGTTTTATAATAATGGAAGTTTAGTTTCTAATGAA
ACTGATCAAACATATATAAGTAGTTTAATGCCAGGAACACTGCACACGAATGCTTATATT
GATGGTGGATCAAGTATAGACAATTCAAGTCAAATATCATTACTTAATGGACATTCTATT
GCCTCTAATGGTTCACATTCAACAACTAATGCATTTATTGAACATAATTTTGATGGTGAA
TGTATGGAGTTAGATGATAATAGAAAATTATCGTTTAGAGGATATATAGGAAGTGGCAAT
TTTGGAGTGGTTTATAAGGGAACTATTGGACCATTAATATTTAATCCTATGGAAGATGAA
GAAGAGGAAGTTGCCATTAAATGCTTTAAACCAATTGATTCATTAAACCAAGCGAAAGAT
TTTCTGCGTGAAGTCAGAATGATGAAAGCTTTAAATCATGAAAATATTGTTAAAATTTAC
GATTTTCATGAAGATTGGCTACTTATAATAATGGAGTATATGTCAGGTGGATCTCTTCAA
GAATTTGTTGCAATACATCGACATGAATTAACAGTTGATGATATACTACAATTTGCTTTA
CATATTGCAAAAGGAATGCACTACTTAGAACAAAATAAAATTGTTCATCGAGATTTGGCT
GCGCGCAATGTGTTAGTGACGAGAAATAGTTCACTTCTATTGTCTGATACTGTTTGTAAA
ATTGCCGATTTTGGTTTGGCTCAATTTACTAATCATTATGGATATTATGAGTCTACAAAT
AACAGAGATCTTCCTCTGCAATGGTATGCTCCAGAAACAATTAGTTGCTTAAAATTTAGC
TCAAAAAATGATGTATGGTCGTTTGGAATAACATTATGGGAAATGTTTTCATTTGGAGAT
ACCCCAAGACTTGTTCCTAAATCAGATTTTAAAGGTGAAGATTTGTTGCAGGCACTTGAA
AAAGGTGAACGTCTTAAATGTCCTAAACATTGTCCTCAAAATATTTATGAAGAACTGATG
CGTGATGTATGTTGGAGTTATAATTCTGATAAAAGGCCAAATTTTGCTGGCATTATTGAA
AAAATTCGAAATCTTTTAATTAGGAATGGTGAATTAGTTTAA
>g4919.t1 Gene=g4919 Length=1153
MWAMETREIKNYILNYKTGKFKEIKHDKDETCEDFCKELCRRWNFPPLVQLLFGLRLHGT
KIWLGSARQLVADKHYEFRIRIKIPKLADLNKHDKNTFDYFYHQVRYDVLHNDIPGLINE
NTRNKILGLCVTDMYVEMLENENKDYPNETSRKKAREEKMKYLNVNYKNYIPRFLYEKHW
VILQMRIKKSLKGVDYKHDPLYVKTSYIQQVDSMAPNYLIEEYWGKIAYPQEDHMRQGTC
RVRLQIAPYDKEQPGLRMHYAYKDTWRHISTFADFYAIQIDADAKQVRLEIQNSPQGFPI
TMDSIEEIESFVNCMNIYYRLTVKWTWYLCELLKSPSLDFLNKYKIHGPIGGGYSYNRIK
EIGKGVGTYIIRQCEKEFDIYYIDILTKKNQSCETFKINGAAEKWQLYDNENNEISAEFD
NLVSLAKSIPVESGVYNRLPPSCYEKPPLLLLCQTNIKSVPASATNTTMITTTQNSPGGL
RTQRPVVFTNEDFRMYIPSTREINDEAFEQRKAEYRDRNTEVTLKLLKTTEKLTEFHLLA
DKWSKLDISEIVKLNGIILNPVALVLEPLKYGPLDIFLRTHEFRRQVVPLNLVETAYSLA
RALHYLQEKQIVHGRIKCSSLEVIKFDPGNSFEVKLGDPGLPRDLKVRDVPWIPIEDYDD
LNNSRNNLKADIWAYATTLWEIFSRGESPFTELSKVPNITEFFRRGDRLPKPKECELLPR
IYEIMKSGWEVEPEKRFAPQTIFSPLLDISRNLSRHYESPISSNPRSNGTMQRMNGGIRN
GRPSSSNSMFYNNGSLVSNETDQTYISSLMPGTLHTNAYIDGGSSIDNSSQISLLNGHSI
ASNGSHSTTNAFIEHNFDGECMELDDNRKLSFRGYIGSGNFGVVYKGTIGPLIFNPMEDE
EEEVAIKCFKPIDSLNQAKDFLREVRMMKALNHENIVKIYDFHEDWLLIIMEYMSGGSLQ
EFVAIHRHELTVDDILQFALHIAKGMHYLEQNKIVHRDLAARNVLVTRNSSLLLSDTVCK
IADFGLAQFTNHYGYYESTNNRDLPLQWYAPETISCLKFSSKNDVWSFGITLWEMFSFGD
TPRLVPKSDFKGEDLLQALEKGERLKCPKHCPQNIYEELMRDVCWSYNSDKRPNFAGIIE
KIRNLLIRNGELV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g4919.t1 | CDD | cd14473 | FERM_B-lobe | 97 | 211 | 5.40464E-6 |
| 13 | g4919.t1 | CDD | cd00192 | PTKc | 875 | 1143 | 1.28556E-100 |
| 11 | g4919.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 509 | 759 | 2.2E-29 |
| 10 | g4919.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 864 | 1147 | 4.0E-74 |
| 3 | g4919.t1 | PANTHER | PTHR45807:SF7 | TYROSINE-PROTEIN KINASE HOPSCOTCH | 16 | 1144 | 2.8E-206 |
| 4 | g4919.t1 | PANTHER | PTHR45807 | TYROSINE-PROTEIN KINASE HOPSCOTCH | 16 | 1144 | 2.8E-206 |
| 5 | g4919.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 951 | 964 | 6.6E-17 |
| 6 | g4919.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 988 | 1006 | 6.6E-17 |
| 7 | g4919.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 1063 | 1085 | 6.6E-17 |
| 2 | g4919.t1 | Pfam | PF07714 | Protein tyrosine and serine/threonine kinase | 514 | 741 | 4.4E-32 |
| 1 | g4919.t1 | Pfam | PF07714 | Protein tyrosine and serine/threonine kinase | 872 | 1141 | 1.4E-79 |
| 15 | g4919.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 876 | 907 | - |
| 14 | g4919.t1 | ProSitePatterns | PS00109 | Tyrosine protein kinases specific active-site signature. | 994 | 1006 | - |
| 18 | g4919.t1 | ProSiteProfiles | PS50057 | FERM domain profile. | 7 | 326 | 17.46 |
| 19 | g4919.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 497 | 747 | 14.662 |
| 20 | g4919.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 870 | 1146 | 35.269 |
| 17 | g4919.t1 | SMART | SM00295 | B41_5 | 3 | 223 | 0.0079 |
| 16 | g4919.t1 | SMART | SM00219 | tyrkin_6 | 870 | 1142 | 6.8E-100 |
| 9 | g4919.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 512 | 757 | 7.57E-26 |
| 8 | g4919.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 868 | 1143 | 6.01E-67 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005856 | cytoskeleton | CC |
| GO:0004713 | protein tyrosine kinase activity | MF |
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K04447 (Janus kinase 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
