General information for g4971.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable cytochrome P450 12a5, mitochondrial.
Parent gene
- The parent gene g4971 is located on chr_2: 6215794..6216554 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4971 | g4971.t3 | TSS | g4971.t3 | 6214628 | 6214628 |
| chr_2 | g4971 | g4971.t3 | isoform | g4971.t3 | 6214668 | 6215918 |
| chr_2 | g4971 | g4971.t3 | exon | g4971.t3.exon1 | 6214668 | 6214963 |
| chr_2 | g4971 | g4971.t3 | exon | g4971.t3.exon2 | 6215019 | 6215248 |
| chr_2 | g4971 | g4971.t3 | cds | g4971.t3.CDS1 | 6215154 | 6215248 |
| chr_2 | g4971 | g4971.t3 | exon | g4971.t3.exon3 | 6215305 | 6215580 |
| chr_2 | g4971 | g4971.t3 | cds | g4971.t3.CDS2 | 6215305 | 6215580 |
| chr_2 | g4971 | g4971.t3 | exon | g4971.t3.exon4 | 6215639 | 6215918 |
| chr_2 | g4971 | g4971.t3 | cds | g4971.t3.CDS3 | 6215639 | 6215816 |
| chr_2 | g4971 | g4971.t3 | TTS | g4971.t3 | 6216616 | 6216616 |
Sequences
>g4971.t3 Gene=g4971 Length=1082
ATGTCAAAAAAAATTTTAGTCCAGCAATCTGTTTTTATCTTTCGACGCTTACTTAATACT
ACGTCTACTTCATTTAACCTTGATGTTCAATCGACTGATGTAAAACCTTTTTCACAAGTT
CCATCATTGTCTGCATTTGAAATCATAGCAAGAAATTTACCAGGTGGTAAATTTTATAAC
AAATCAATGAAAGATTTGCACTTAATAATGAATGCTGAGTTCGGCAATATAGTTCGATTT
GCTGGTATATTTGGCCGACCACCATTATTATTTGTTTATGATGCAAATGACTTCGAACAT
TGAGAACTGAAGGTGTATGGCCAGAAAGAATTGCATTAGAATCACTTGCTTACTATAGGA
AAACAAAGCGACCAGATATTTACAATGAATATTCTGGCTTGTTTTCAGCTCAGGGAGAAG
AATGGCATAAAATGAGAACAATAACTAATCCGATTTTGATGCAACCAAAAATTATTAAAC
TTTATTGCAAACAAGTTGATGAAATAGCAAAGGAATTTGTTGGAATCATGCGGAAATTAA
GGGATGAGAAATGTGAAATGCCTGCTGATTTTAGCAATTACATAAATTCATGGTCGTTAG
AGTCTATTGTTGCTATTGCTTTAGAAAGACGTTTAAATATACTGAGTGGGTCCTCAAATG
ATGAAAAAGCTCAAGAGCTCATCAAAAATATTCGTCTCTTCTTTGAAAAATCTTTCCTTT
ATGATGTACTTCCTTCAGTTTGGCGTTACTATGAAACGAAAGGTTTCAAGAATTTCCTGA
AAGTATATGATACAATAACAAACATTGTGCTTCACTACATAGATATTGCAATAAAGGAAA
TGGAAAAAAGTGAGTCATCTTCTTTGAATCATGAAGATAGCATATTACGGAAACTTTTGA
AGATTGACAAAAAAGCGGCAGTTGTGATGGCGAGTGATATGTTGTTAGGTAAAAAGATCT
CAAATGTGAAATTCATTTGATTGAAATTTTTTATTCTTATTTATCCATTTAGCTGGTGTT
GATACAGTAAGATGTTATATACATGTAAAATCATAACTAAAGAAAAATATTTTTTTATAA
AA
>g4971.t3 Gene=g4971 Length=182
MRTITNPILMQPKIIKLYCKQVDEIAKEFVGIMRKLRDEKCEMPADFSNYINSWSLESIV
AIALERRLNILSGSSNDEKAQELIKNIRLFFEKSFLYDVLPSVWRYYETKGFKNFLKVYD
TITNIVLHYIDIAIKEMEKSESSSLNHEDSILRKLLKIDKKAAVVMASDMLLGKKISNVK
FI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g4971.t3 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 1 | 174 | 0 |
| 2 | g4971.t3 | PANTHER | PTHR24291:SF155 | CYTOCHROME P450 301B1 | 2 | 173 | 0 |
| 3 | g4971.t3 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 2 | 173 | 0 |
| 1 | g4971.t3 | Pfam | PF00067 | Cytochrome P450 | 1 | 157 | 0 |
| 4 | g4971.t3 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 2 | 158 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
