General information for g4991.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Peroxisomal membrane protein PEX14.
Parent gene
- The parent gene g4991 is located on chr_2: 6340521..6340963 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4991 | g4991.t1 | TSS | g4991.t1 | 6339886 | 6339886 |
| chr_2 | g4991 | g4991.t1 | isoform | g4991.t1 | 6339996 | 6340963 |
| chr_2 | g4991 | g4991.t1 | exon | g4991.t1.exon1 | 6339996 | 6340019 |
| chr_2 | g4991 | g4991.t1 | cds | g4991.t1.CDS1 | 6339996 | 6340019 |
| chr_2 | g4991 | g4991.t1 | exon | g4991.t1.exon2 | 6340078 | 6340392 |
| chr_2 | g4991 | g4991.t1 | cds | g4991.t1.CDS2 | 6340078 | 6340392 |
| chr_2 | g4991 | g4991.t1 | exon | g4991.t1.exon3 | 6340511 | 6340963 |
| chr_2 | g4991 | g4991.t1 | cds | g4991.t1.CDS3 | 6340511 | 6340963 |
| chr_2 | g4991 | g4991.t1 | TTS | g4991.t1 | 6341147 | 6341147 |
Sequences
>g4991.t1 Gene=g4991 Length=792
ATGGATGTCCCTCGTGAATCGGCAATATCGACTGCTGTAAATTTTTTAAGAAATAAAAAC
GTATCGAGAAGTAGTATTTTACAAAAAAGAAACTTTCTGGCCAGTAAAGGCCTTACTGAC
GAGGAAATACAGCAAGCATTTGAAAGAGTCGGAATATTTGCCAAAGTTAATACACTTGAT
AGTGCAATGGACGAAACAACTATAAATATCCCACAATCACAACCACTTGTACATCATTCC
TACAAGCATCAAATGACAACATTTGAAAAAGTAAAAGATGTTTTGAGTTCTATGGCATTG
ATCTCGGGAATTGCATACGCGATTTACATGTTTTATAAGAAATATATTGAGCCATTTTTA
TTCGGAAGAAAGAAGAAGGGCGATAAAATAGACGAACTTGCTAAAACAACAAAAGCAATA
AATGGAGAACTTATCAGAGTGCGAGAAGAGATAAATCTTCAAAAACAAACACAGGAATCT
TCAAACAAACTTATTCAAAACACACTGCAAACGTTCAAAACAGAGATTGAAACGATCAAA
GGCATTTTGCTGAATCATAGAAATTTTTCCTCTGCTCCGTCTTCCTCTGGCATCAATTTA
CCGAAAATTCCCTCATGGCAATTGAGCAGTAACAAATCGAAGAATGATGAAAAGCGAAGT
GATCATGATGATGATAAACATTCAAACTCAAGTGAAAACGAGACAGAGGTCATTGTCGAG
GAAAAAAACCAGAAACAGAACCAGAAACAGCGATCTCCGGCCAATAGTGATTCTTCGCTT
GAAATTATGTAA
>g4991.t1 Gene=g4991 Length=263
MDVPRESAISTAVNFLRNKNVSRSSILQKRNFLASKGLTDEEIQQAFERVGIFAKVNTLD
SAMDETTINIPQSQPLVHHSYKHQMTTFEKVKDVLSSMALISGIAYAIYMFYKKYIEPFL
FGRKKKGDKIDELAKTTKAINGELIRVREEINLQKQTQESSNKLIQNTLQTFKTEIETIK
GILLNHRNFSSAPSSSGINLPKIPSWQLSSNKSKNDEKRSDHDDDKHSNSSENETEVIVE
EKNQKQNQKQRSPANSDSSLEIM
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g4991.t1 | Gene3D | G3DSA:1.10.10.10 | winged helix repressor DNA binding domain | 1 | 58 | 2.4E-20 |
| 5 | g4991.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 194 | 263 | - |
| 6 | g4991.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 194 | 211 | - |
| 4 | g4991.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 212 | 247 | - |
| 7 | g4991.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 248 | 263 | - |
| 2 | g4991.t1 | PANTHER | PTHR23058 | PEROXISOMAL MEMBRANE PROTEIN PEX14 | 4 | 252 | 1.7E-24 |
| 1 | g4991.t1 | Pfam | PF04695 | Pex14 N-terminal domain | 5 | 49 | 6.9E-14 |
| 10 | g4991.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 93 | - |
| 11 | g4991.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 94 | 112 | - |
| 9 | g4991.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 113 | 263 | - |
| 3 | g4991.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 94 | 112 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
| GO:0016560 | protein import into peroxisome matrix, docking | BP |
| GO:0005778 | peroxisomal membrane | CC |
KEGG
Orthology
- This transcript has been annotated with K13343 (peroxin-14)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
