General information for g5012.t8
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as hypothetical.
Parent gene
- The parent gene g5012 is located on chr_2: 6463528..6463962 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5012 | g5012.t8 | TSS | g5012.t8 | 6444628 | 6444628 |
| chr_2 | g5012 | g5012.t8 | isoform | g5012.t8 | 6445003 | 6452748 |
| chr_2 | g5012 | g5012.t8 | exon | g5012.t8.exon1 | 6445003 | 6445052 |
| chr_2 | g5012 | g5012.t8 | cds | g5012.t8.CDS1 | 6445003 | 6445052 |
| chr_2 | g5012 | g5012.t8 | exon | g5012.t8.exon2 | 6449432 | 6449697 |
| chr_2 | g5012 | g5012.t8 | cds | g5012.t8.CDS2 | 6449432 | 6449697 |
| chr_2 | g5012 | g5012.t8 | exon | g5012.t8.exon3 | 6450668 | 6450698 |
| chr_2 | g5012 | g5012.t8 | cds | g5012.t8.CDS3 | 6450668 | 6450698 |
| chr_2 | g5012 | g5012.t8 | exon | g5012.t8.exon4 | 6451055 | 6451099 |
| chr_2 | g5012 | g5012.t8 | cds | g5012.t8.CDS4 | 6451055 | 6451099 |
| chr_2 | g5012 | g5012.t8 | exon | g5012.t8.exon5 | 6451173 | 6451207 |
| chr_2 | g5012 | g5012.t8 | cds | g5012.t8.CDS5 | 6451173 | 6451207 |
| chr_2 | g5012 | g5012.t8 | exon | g5012.t8.exon6 | 6451668 | 6451763 |
| chr_2 | g5012 | g5012.t8 | cds | g5012.t8.CDS6 | 6451668 | 6451763 |
| chr_2 | g5012 | g5012.t8 | exon | g5012.t8.exon7 | 6451823 | 6451848 |
| chr_2 | g5012 | g5012.t8 | cds | g5012.t8.CDS7 | 6451823 | 6451848 |
| chr_2 | g5012 | g5012.t8 | exon | g5012.t8.exon8 | 6452048 | 6452085 |
| chr_2 | g5012 | g5012.t8 | cds | g5012.t8.CDS8 | 6452048 | 6452085 |
| chr_2 | g5012 | g5012.t8 | exon | g5012.t8.exon9 | 6452612 | 6452748 |
| chr_2 | g5012 | g5012.t8 | cds | g5012.t8.CDS9 | 6452612 | 6452747 |
| chr_2 | g5012 | g5012.t8 | TTS | g5012.t8 | NA | NA |
Sequences
>g5012.t8 Gene=g5012 Length=724
ATGGGCTCAAGACAAGTGGTAGCCAATAAAATGGCAAATCCACAATCATGGTGGTACTTA
GCATTATTAATCTCATTTATGATTGTAAATACAAGTGCTCAAATGTGGAATGAAGTAGAG
AGCAGTGGTATAGGATTAACAGGAAGATCGAGGAATAGGCAGCCATTTAGAGAAAATGTA
CAAAGAGTGCCAGACTCTTATGATATTGTAGACACTGCCAGTGCACAAGTCAGAGGCCCA
CCTGCTAAAAATTGTACAGGCTCTGGTTGTTGTATACCAAAATGTTTTGCAGAAAAAGGA
AATAGAGGATTACCAGGAGTTCCTGGATATCCTGGGCCCAAAGTTAAGTATGCAAGGATT
TGCGGGTTCCGAAGGTCTACCTGGAATGAAAGGACAAAAGGGGGAACCAGGTCCGACTGG
CCCAATGAATTAAGAAAAGAAATGTTGCTAATTAATGAATATTTATTAGGAATTAATGGA
CAAATGGGTCCTCCTGGAATGCCCGGACTTCCTGGACTTGATGGTTGTAATGGAACTGAT
GGATTACCTGGGCTTCCGGGATTACAAGGAAATCCAGGCCCAAGAGGTTATCCTGGACCA
TCGGGTATTAAAGGAGAAAAGGGTGAACCTGCAAATATAGACTATACAAATTATGAAAAA
GGACAAAAGGGTGAACCAGGATTTGACGGAAGAATCGGAAATCCCGGTTTGCCAGGCCAG
CCAG
>g5012.t8 Gene=g5012 Length=241
MGSRQVVANKMANPQSWWYLALLISFMIVNTSAQMWNEVESSGIGLTGRSRNRQPFRENV
QRVPDSYDIVDTASAQVRGPPAKNCTGSGCCIPKCFAEKGNRGLPGVPGYPGPKVKYARI
CGFRRSTWNERTKGGTRSDWPNELRKEMLLINEYLLGINGQMGPPGMPGLPGLDGCNGTD
GLPGLPGLQGNPGPRGYPGPSGIKGEKGEPANIDYTNYEKGQKGEPGFDGRIGNPGLPGQ
P
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g5012.t8 | MobiDBLite | mobidb-lite | consensus disorder prediction | 191 | 241 | - |
| 2 | g5012.t8 | PANTHER | PTHR24023:SF1005 | COLLAGEN ALPHA-1(IV) CHAIN | 82 | 240 | 2.8E-16 |
| 3 | g5012.t8 | PANTHER | PTHR24023 | COLLAGEN ALPHA | 82 | 240 | 2.8E-16 |
| 1 | g5012.t8 | Pfam | PF01391 | Collagen triple helix repeat (20 copies) | 157 | 210 | 1.7E-10 |
| 8 | g5012.t8 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 15 | - |
| 9 | g5012.t8 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 16 | 33 | - |
| 7 | g5012.t8 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 34 | 241 | - |
| 6 | g5012.t8 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 33 | - |
| 4 | g5012.t8 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 17 | 36 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5012/g5012.t8; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5012.t8.fa.iupred3.txt does not exist
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
