General information for g5015.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Venom dipeptidyl peptidase 4.
Parent gene
- The parent gene g5015 is located on chr_2: 6469830..6470734 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5015 | g5015.t4 | isoform | g5015.t4 | 6469369 | 6470734 |
| chr_2 | g5015 | g5015.t4 | exon | g5015.t4.exon1 | 6469369 | 6469370 |
| chr_2 | g5015 | g5015.t4 | exon | g5015.t4.exon2 | 6469432 | 6470154 |
| chr_2 | g5015 | g5015.t4 | cds | g5015.t4.CDS1 | 6469432 | 6470154 |
| chr_2 | g5015 | g5015.t4 | exon | g5015.t4.exon3 | 6470278 | 6470523 |
| chr_2 | g5015 | g5015.t4 | cds | g5015.t4.CDS2 | 6470278 | 6470523 |
| chr_2 | g5015 | g5015.t4 | exon | g5015.t4.exon4 | 6470732 | 6470734 |
| chr_2 | g5015 | g5015.t4 | cds | g5015.t4.CDS3 | 6470732 | 6470734 |
| chr_2 | g5015 | g5015.t4 | TSS | g5015.t4 | 6470866 | 6470866 |
| chr_2 | g5015 | g5015.t4 | TTS | g5015.t4 | NA | NA |
Sequences
>g5015.t4 Gene=g5015 Length=974
ATGCTAAAGCTTTTGATTGTTTTATTGGTTTATGTGACTGCGAGTGATGCACGAACAATA
AAAAATTTGGCAGATGGTGGTCGATCAGTTTGGTTTAATGAAACAATTCCAAGTAGTTCT
TTTGGAGCAAGTGGTTTTGGTGGCACGTGGATTTCGGGAAATGAATTTACATACAGTTCA
AATAGGAACATGATTAAGCATAATATTATTACGAGAGTGAATGAAACATTACTTGATAGC
ACTTTTTCGGCAAGATGGAGTGGAGCATCATTAACATTATCTCCAGGAAGAACAAAAGTT
TTAGTTCGATATGCACAACGATCAATTTTTAGACATAGCACAGTTGCAAAGTTTTCAGTG
GTTTTTATGGACAATTTAGAAGTTGAATATAAGATTAGTGATGGTGATGAGATTCAATTA
TGCAAATTTTCACCAAATGATGATGGACTTATTTATATTAAACAAAATACAATTTATCAT
GCTACTGATGAACAAATTAAGGCAGGAAGATATTCAGTACAGTCACCACCCGGAGAAGAT
GGAGTTTTATATTTTGGAATTCCAGATTGGGTTTATGAAGAAGAAGTTTTAGGAAGTGAT
GCAGCAGCTTGGTTTTCAAAAGATGGAAAATATCTCGCTTATGCATTTTTTGATGATACA
AATGTAAAAGATTTCATGTATGAGTTATATGGAGATGGTAAAAATGAATATCAATATCCA
AAAGAAGTTCATTTAAGATATCCAAAAGTTGGTGAAGCAAATCCGAGAGTATCACTTTTA
GTCAATGATCTTACTGATTCATTTGGAACACCATTTTCACTGAATTATCCAGAAAAAGTA
ACAACTGATCATATTCTTGGAACAGTCTTTTGGATTACTGATACAAAATTAGGAGCAATT
TGGCTCAATCGTCGCCAAAATTATGGAGTTTTCGTTTCATATTTAACTGATGATAATTGG
AGAATGGAAGAGAT
>g5015.t4 Gene=g5015 Length=324
MLKLLIVLLVYVTASDARTIKNLADGGRSVWFNETIPSSSFGASGFGGTWISGNEFTYSS
NRNMIKHNIITRVNETLLDSTFSARWSGASLTLSPGRTKVLVRYAQRSIFRHSTVAKFSV
VFMDNLEVEYKISDGDEIQLCKFSPNDDGLIYIKQNTIYHATDEQIKAGRYSVQSPPGED
GVLYFGIPDWVYEEEVLGSDAAAWFSKDGKYLAYAFFDDTNVKDFMYELYGDGKNEYQYP
KEVHLRYPKVGEANPRVSLLVNDLTDSFGTPFSLNYPEKVTTDHILGTVFWITDTKLGAI
WLNRRQNYGVFVSYLTDDNWRMEE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g5015.t4 | Gene3D | G3DSA:2.140.10.30 | - | 31 | 323 | 2.2E-72 |
| 2 | g5015.t4 | PANTHER | PTHR11731 | PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED | 6 | 313 | 3.0E-81 |
| 3 | g5015.t4 | PANTHER | PTHR11731:SF154 | VENOM DIPEPTIDYL PEPTIDASE 4-LIKE PROTEIN | 6 | 313 | 3.0E-81 |
| 1 | g5015.t4 | Pfam | PF00930 | Dipeptidyl peptidase IV (DPP IV) N-terminal region | 94 | 314 | 2.7E-49 |
| 8 | g5015.t4 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 9 | g5015.t4 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 10 | g5015.t4 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 12 | - |
| 11 | g5015.t4 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 17 | - |
| 7 | g5015.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 324 | - |
| 4 | g5015.t4 | SUPERFAMILY | SSF82171 | DPP6 N-terminal domain-like | 28 | 314 | 3.01E-23 |
| 5 | g5015.t4 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5015/g5015.t4; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5015.t4.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
