General information for g5057.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Disintegrin and metalloproteinase domain-containing protein 10-like protein.
Parent gene
- The parent gene g5057 is located on chr_2: 6638513..6639971 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5057 | g5057.t1 | isoform | g5057.t1 | 6632457 | 6639377 |
| chr_2 | g5057 | g5057.t1 | exon | g5057.t1.exon1 | 6632457 | 6632493 |
| chr_2 | g5057 | g5057.t1 | cds | g5057.t1.CDS1 | 6632457 | 6632493 |
| chr_2 | g5057 | g5057.t1 | exon | g5057.t1.exon2 | 6633357 | 6633487 |
| chr_2 | g5057 | g5057.t1 | cds | g5057.t1.CDS2 | 6633357 | 6633487 |
| chr_2 | g5057 | g5057.t1 | exon | g5057.t1.exon3 | 6633558 | 6634588 |
| chr_2 | g5057 | g5057.t1 | cds | g5057.t1.CDS3 | 6633558 | 6634588 |
| chr_2 | g5057 | g5057.t1 | exon | g5057.t1.exon4 | 6634649 | 6634899 |
| chr_2 | g5057 | g5057.t1 | cds | g5057.t1.CDS4 | 6634649 | 6634899 |
| chr_2 | g5057 | g5057.t1 | exon | g5057.t1.exon5 | 6634955 | 6635265 |
| chr_2 | g5057 | g5057.t1 | cds | g5057.t1.CDS5 | 6634955 | 6635265 |
| chr_2 | g5057 | g5057.t1 | exon | g5057.t1.exon6 | 6635490 | 6636095 |
| chr_2 | g5057 | g5057.t1 | cds | g5057.t1.CDS6 | 6635490 | 6636095 |
| chr_2 | g5057 | g5057.t1 | exon | g5057.t1.exon7 | 6636198 | 6636391 |
| chr_2 | g5057 | g5057.t1 | cds | g5057.t1.CDS7 | 6636198 | 6636391 |
| chr_2 | g5057 | g5057.t1 | exon | g5057.t1.exon8 | 6636702 | 6636719 |
| chr_2 | g5057 | g5057.t1 | cds | g5057.t1.CDS8 | 6636702 | 6636719 |
| chr_2 | g5057 | g5057.t1 | exon | g5057.t1.exon9 | 6639311 | 6639377 |
| chr_2 | g5057 | g5057.t1 | cds | g5057.t1.CDS9 | 6639311 | 6639377 |
| chr_2 | g5057 | g5057.t1 | TSS | g5057.t1 | 6639965 | 6639965 |
| chr_2 | g5057 | g5057.t1 | TTS | g5057.t1 | NA | NA |
Sequences
>g5057.t1 Gene=g5057 Length=2646
ATGAAATCAAAATGTAGATTGATTGCAATTTGGTTAATGATTTCATTTAATTCGTTTATC
GAAACTGGATATGTCAAAGAAAATGGCAAACGTTTGAATGAATACATATCGCATTTTGAG
ACATTAAGTTACGATACAAAACATTTGCATGCAAAGCATAATCGTGCAAAGCGTTCCATC
GGCGAAGATCAGCATGTTCATTTAAAATTCTCAGCTCATGGTGAAACTTTTCATCTTAGA
CTTAAACGCGATTTGACTACATTTAGTGATAATTTAGAGATTCATCATACAGATAATGGC
GTAGAGAGTAAAAGGCTTTTAGATACAAGTCACATATATGAGGGTCATGTGCTTGGTGAT
AAAGACAGTTATGTTCATGGCTCGATAAGTGATGGAGTTTTCCATGGACGAATTATTACA
AATAAAGACTCATATTTTGTGGAAAAAGCTCAATATTATTTTCCTAATCATAGTCATGTA
GAAAGCGGATTTCATTCCATAATTTACAAAGACCAACATGTCGATGATCCTTATCGATTT
AATCGCACAGTTCATCCAAATGGCTGTGGACTAACCGATGATGTTGCACATTGGATGGAT
CGAGTACAAAGTTCTGCCGATGTGCTTGATGAAGAAATTGAAATTGTTCAATTGCAACAG
CAGCAACAGCAGAAAGAAAAGGAAAAGAAAGATGATAGCAAAAGTCACTCTTATCAGCAT
GTAAATAATCAAATTTATGATGAGAGTGCATATGAAAAATACTCCAAAGAAGCCAATTCA
CGGCCAAAGCGTGCAACTGTTCGTTACCAAGAGAAAAATACTTGCTCGCTTTATATTCAA
ACTGATCCACTCATTTGGCGCCATATTCGTGAATCCATTCCAGATCACAATGATCCAAAT
CGAGCTGCAATTGTCGATGAGAAAGCAAAGGAAGAGATTCTTTCACTTGTCGCTCATCAC
GTTGCTGCTGTAAATTTCATTTATCGTAATACAAAATTTGATGGACGAGTTGAGCATCGA
AATATTCGTTTTGAGGTGCAGCGAATAAAAATCGATGATGATTCAGCATGTAGTATTTAC
TATCGTGGTGAACCAAATCAATTTTGTATGGAAAATATTGATGTCTCAAATTTCCTCAAT
CTTCATTCACTTGGAAATCATGAGCATTTTTGTTTAGCTTATGTTTTTACTTATCGTGAT
TTCACTGGAGGTACACTTGGTCTAGCATGGGTTGCAAGTGCTTCTGGCGCGTCTGGTGGA
ATATGTGAAAAATACAAGTCCTATACTGAAACAACAAGTCAAGGAATGTATCATAGCACA
AAAAGATCTCTCAATACTGGTATTATTACATTTGTCAACTACAATTCTCGTGTGCCACCT
AAAGTATCACAATTGACACTTGCTCATGAGATTGGACATAATTTTGGATCACCACATGAT
TATCCACCTGAGTGTAGACCAGGAGGACAACCAGGAAATTATATAATGTTTTCATCAGCA
ACAAGTGGTGATCGTCCAAATAATAGCAAATTTTCACAATGTTCAATAAGAAATATTTCC
AATGTTCTTGATGCAATTGATGAAGCCAAAAAACGTAATTGCTTTAAAGTCAGTGAAGGA
GCATTTTGTGGAAATAAAATTGTTGAAATTGGTGAAGAATGTGATTGTGGATTCAATGAT
GAAGAATGTCGTGATAGATGCTGTTATCCACGAATTGTTTCTGATTATGATCTTAATCTC
AATGCATCAGCTAAAGGTTGTAGTCGTCGTGCAAAGACACAATGCAGTCCAAGTCAAGGA
CCATGCTGTGATCCAAATAGTTGTACATTTGTGCCGATCAACAAACGAGCCAAGTGCCGT
GAAGAGTCAGAATGTTCATGGAGTTCTATGTGTAATGGAACAACCAGTGAATGTCCTGAT
CCAAAACCAAGAGAAGACAAAACAAAATGCAACAATGGCACACAACTTTGCATTAGTGGT
GAATGCACTGGATCGATTTGTCTCAGTTGGAATAAAACCGAGTGCTTCCTTTCATCAACA
CAGACCAATTATGACAAGCGACGATTATGTGAGCTTGCATGTCAAGACGGTAATGACGTT
TCAACATGTCGTTCGACAAGTGAATTTGGTCATTTATATGGATTGCCTAAGGGTGGCATA
AGTTTGCGACCCGGGTCGCCATGTGACAACTTTCAAGGCTATTGTGATGTATTCTTAAAG
TGCCGAGCTGTGGATGCTGATGGTCCATTGGTACGACTTAAAAATCTTTTACTCAACAGA
GAGACACTGCACACATTGAAGCAATATGTCATAGAAAATTGGTACTTTTGCGTTCTTATG
GCCATCATTTTCTTCATCTTCATGGGCATATTTATCAAATGCTGTGCCGTACACACGCCA
AGTAGTAATCCCAAAAAAGCACCTGCACGTAGAATCAGTGACACTTTGAGACGACCCATG
AATACATTAAGAAGAATGCGTCATCCTGCAAATGTAGCAAGAAGTATTCCACCGCCTGGA
CATGGAGAAAGACGAAATCGAAGTAGAAGCGGTGGAAATCGTCAAGCCGGCAGTAGTGGT
GGACAGAGGCCAATTTCACATCATGGAGGTCGACGCCCCGTTAATTCCAGTAGCACAGGG
TTATAG
>g5057.t1 Gene=g5057 Length=881
MKSKCRLIAIWLMISFNSFIETGYVKENGKRLNEYISHFETLSYDTKHLHAKHNRAKRSI
GEDQHVHLKFSAHGETFHLRLKRDLTTFSDNLEIHHTDNGVESKRLLDTSHIYEGHVLGD
KDSYVHGSISDGVFHGRIITNKDSYFVEKAQYYFPNHSHVESGFHSIIYKDQHVDDPYRF
NRTVHPNGCGLTDDVAHWMDRVQSSADVLDEEIEIVQLQQQQQQKEKEKKDDSKSHSYQH
VNNQIYDESAYEKYSKEANSRPKRATVRYQEKNTCSLYIQTDPLIWRHIRESIPDHNDPN
RAAIVDEKAKEEILSLVAHHVAAVNFIYRNTKFDGRVEHRNIRFEVQRIKIDDDSACSIY
YRGEPNQFCMENIDVSNFLNLHSLGNHEHFCLAYVFTYRDFTGGTLGLAWVASASGASGG
ICEKYKSYTETTSQGMYHSTKRSLNTGIITFVNYNSRVPPKVSQLTLAHEIGHNFGSPHD
YPPECRPGGQPGNYIMFSSATSGDRPNNSKFSQCSIRNISNVLDAIDEAKKRNCFKVSEG
AFCGNKIVEIGEECDCGFNDEECRDRCCYPRIVSDYDLNLNASAKGCSRRAKTQCSPSQG
PCCDPNSCTFVPINKRAKCREESECSWSSMCNGTTSECPDPKPREDKTKCNNGTQLCISG
ECTGSICLSWNKTECFLSSTQTNYDKRRLCELACQDGNDVSTCRSTSEFGHLYGLPKGGI
SLRPGSPCDNFQGYCDVFLKCRAVDADGPLVRLKNLLLNRETLHTLKQYVIENWYFCVLM
AIIFFIFMGIFIKCCAVHTPSSNPKKAPARRISDTLRRPMNTLRRMRHPANVARSIPPPG
HGERRNRSRSGGNRQAGSSGGQRPISHHGGRRPVNSSSTGL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g5057.t1 | Coils | Coil | Coil | 206 | 228 | - |
| 10 | g5057.t1 | Gene3D | G3DSA:3.40.390.10 | Collagenase (Catalytic Domain) | 269 | 537 | 1.3E-81 |
| 9 | g5057.t1 | Gene3D | G3DSA:4.10.70.10 | Echistatin | 540 | 641 | 3.2E-13 |
| 24 | g5057.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 220 | 242 | - |
| 23 | g5057.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 223 | 237 | - |
| 22 | g5057.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 805 | 881 | - |
| 4 | g5057.t1 | PANTHER | PTHR45702 | ADAM10/ADAM17 METALLOPEPTIDASE FAMILY MEMBER | 5 | 830 | 2.0E-268 |
| 5 | g5057.t1 | PANTHER | PTHR45702:SF2 | KUZBANIAN, ISOFORM A | 5 | 830 | 2.0E-268 |
| 3 | g5057.t1 | Pfam | PF01562 | Reprolysin family propeptide | 41 | 156 | 5.5E-11 |
| 1 | g5057.t1 | Pfam | PF13574 | Metallo-peptidase family M12B Reprolysin-like | 319 | 523 | 9.0E-28 |
| 2 | g5057.t1 | Pfam | PF00200 | Disintegrin | 549 | 640 | 7.5E-12 |
| 14 | g5057.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 15 | g5057.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 16 | g5057.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 14 | - |
| 18 | g5057.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 18 | - |
| 13 | g5057.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 772 | - |
| 17 | g5057.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 773 | 796 | - |
| 12 | g5057.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 797 | 881 | - |
| 26 | g5057.t1 | ProSiteProfiles | PS50215 | ADAM type metalloprotease domain profile. | 273 | 523 | 16.177 |
| 25 | g5057.t1 | ProSiteProfiles | PS50214 | Disintegrin domain profile. | 540 | 646 | 12.988 |
| 21 | g5057.t1 | SMART | SM00050 | DISIN_2 | 549 | 644 | 9.6E-10 |
| 7 | g5057.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 271 | 524 | 5.06E-43 |
| 6 | g5057.t1 | SUPERFAMILY | SSF57552 | Blood coagulation inhibitor (disintegrin) | 549 | 641 | 1.62E-6 |
| 8 | g5057.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
| 19 | g5057.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 25 | - |
| 20 | g5057.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 770 | 792 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5057/g5057.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5057.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0004222 | metalloendopeptidase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K06704 (disintegrin and metalloproteinase domain-containing protein 10)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
