General information for g5091.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine/threonine-protein kinase grp.
Parent gene
- The parent gene g5091 is located on chr_2: 6844251..6844872 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5091 | g5091.t3 | TSS | g5091.t3 | 6839112 | 6839112 |
| chr_2 | g5091 | g5091.t3 | isoform | g5091.t3 | 6839180 | 6841366 |
| chr_2 | g5091 | g5091.t3 | exon | g5091.t3.exon1 | 6839180 | 6839182 |
| chr_2 | g5091 | g5091.t3 | exon | g5091.t3.exon2 | 6840237 | 6840592 |
| chr_2 | g5091 | g5091.t3 | cds | g5091.t3.CDS1 | 6840504 | 6840592 |
| chr_2 | g5091 | g5091.t3 | exon | g5091.t3.exon3 | 6840681 | 6840904 |
| chr_2 | g5091 | g5091.t3 | cds | g5091.t3.CDS2 | 6840681 | 6840904 |
| chr_2 | g5091 | g5091.t3 | exon | g5091.t3.exon4 | 6840991 | 6841117 |
| chr_2 | g5091 | g5091.t3 | cds | g5091.t3.CDS3 | 6840991 | 6841117 |
| chr_2 | g5091 | g5091.t3 | exon | g5091.t3.exon5 | 6841175 | 6841366 |
| chr_2 | g5091 | g5091.t3 | cds | g5091.t3.CDS4 | 6841175 | 6841364 |
| chr_2 | g5091 | g5091.t3 | TTS | g5091.t3 | 6842206 | 6842206 |
Sequences
>g5091.t3 Gene=g5091 Length=902
TAGATTTTTCTGTTTGCTGTATTTTTTCTAACACCTATCAAAAATATTTTTAAAGTTTTT
GATTATATGACGTAAAATCAAACTGAATTTTTAATCTAAATGAATCGAATAGAATAAGTA
TCTATTTGTGAAGGATAAAACAATGCAGATGATGCTTCAATATACCTCGTCAAAACGATC
AAAAAAATCTTAAGTGTTCAAAACTTCTATTTTTAAATATACATTAATTTTAAGACCAAA
AGTACCTAAAATATAGATATATAAGTTAAAATGACATCACAAGGCTCGAGCGAGGTAACT
CGGGAATTTGTTGAAAATTGGAATCTATGTCAGACCCTTGGAGAAGGAGCTTATGGAGAA
GTTAAACTTTTAATTAATAAAAACACGAGTGAAGCTATAGCTATGAAAATGGTAGATTTA
GAGAGACATCCAGATGCTATGGCATCAGTTAAGAAAGAAGTATGCATACAGAAAATTCTC
TCTCATAAGCACATTTTAAAGTTCTATGGCAAACGCTCACAAAACAATATTGAGTACATA
TTTCTTGAATATGCAGCGGGTGGAGAGTTGTTTGATCGAATCGAACCTGATGTTGGCATG
GAAATTACTCAAGCTCAACGATATTTCAATCAAATATTAGCCGGTGTTGAATATTTACAC
AAAAGAGGAATAGCACATCGAGATTTGAAACCTGAAAATATATTAATAGATGATCATAAT
AATATAAAGATTTGCGATTTTGGAATGGCCACAGTGTACAGATTAAAAGGTCGAGAACGA
TTGCTGGACAAGAAATGTGGAACAATGCCTTATGTTGCGCCTGAAGTACTTGTAAAACCA
TACCATGCTGAGCCTGCTGATCTTTGGGCATGTGGCATCATTCTAGTGACTATGCTTGGT
GG
>g5091.t3 Gene=g5091 Length=210
MTSQGSSEVTREFVENWNLCQTLGEGAYGEVKLLINKNTSEAIAMKMVDLERHPDAMASV
KKEVCIQKILSHKHILKFYGKRSQNNIEYIFLEYAAGGELFDRIEPDVGMEITQAQRYFN
QILAGVEYLHKRGIAHRDLKPENILIDDHNNIKICDFGMATVYRLKGRERLLDKKCGTMP
YVAPEVLVKPYHAEPADLWACGIILVTMLG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g5091.t3 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 9 | 100 | 2.1E-28 |
| 9 | g5091.t3 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 101 | 210 | 2.6E-37 |
| 2 | g5091.t3 | PANTHER | PTHR24346 | MAP/MICROTUBULE AFFINITY-REGULATING KINASE | 14 | 209 | 4.6E-70 |
| 3 | g5091.t3 | PANTHER | PTHR24346:SF42 | SERINE/THREONINE-PROTEIN KINASE SIK3 | 14 | 209 | 4.6E-70 |
| 1 | g5091.t3 | Pfam | PF00069 | Protein kinase domain | 20 | 209 | 2.3E-56 |
| 7 | g5091.t3 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 23 | 46 | - |
| 6 | g5091.t3 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 134 | 146 | - |
| 10 | g5091.t3 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 17 | 210 | 40.626 |
| 5 | g5091.t3 | SMART | SM00220 | serkin_6 | 17 | 210 | 3.8E-42 |
| 4 | g5091.t3 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 9 | 209 | 9.58E-62 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5091/g5091.t3; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5091.t3.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
