General information for g51.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 6A1.
Parent gene
- The parent gene g51 is located on chr_3: 419293..421130 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g51 | g51.t3 | TSS | g51.t3 | 419263 | 419263 |
| chr_3 | g51 | g51.t3 | isoform | g51.t3 | 419293 | 420349 |
| chr_3 | g51 | g51.t3 | exon | g51.t3.exon1 | 419293 | 419665 |
| chr_3 | g51 | g51.t3 | cds | g51.t3.CDS1 | 419293 | 419665 |
| chr_3 | g51 | g51.t3 | exon | g51.t3.exon2 | 419735 | 420287 |
| chr_3 | g51 | g51.t3 | cds | g51.t3.CDS2 | 419735 | 420287 |
| chr_3 | g51 | g51.t3 | exon | g51.t3.exon3 | 420345 | 420349 |
| chr_3 | g51 | g51.t3 | cds | g51.t3.CDS3 | 420345 | 420348 |
| chr_3 | g51 | g51.t3 | TTS | g51.t3 | NA | NA |
Sequences
>g51.t3 Gene=g51 Length=931
ATGTCATCGTTAGAAGTATTTTTAGCGTTATTTTTAGTAGCTTTAGGGCTATGCGTGTTT
TTTATCAAGAAAAAATTTTCTTATTGGAAAGAACGCGGTTTCGATTACATCGAGCCTGAA
TTTCCATTTGGTAATTTAAAGGGCGTTGGCTACAAAATTCACTTTTCAGAATTATCTCGT
GAATATTATGAGCGTTATAAAAATAAAACGTCGGCAATCGGCCTTTATTTTTTCACACAA
CCGGTTGTATTTTTAACGAATTTGGACGCCGTAAAAAATATTCTGGTAAAAGACTTTTAT
AATTTTCATGATCGCGGTCTTTATGTCAACACAAAGTCAGATCCACTTTCAGCACATTTG
TTTGCTATTGAGGGAGCAGAATGGAAACGTATGCGAACCAAATTATCACCAACTTTTACG
AGCATGAGAATGAAAACGATGTTTGGAACGGTACTGAATATTTCTGAGGAAATGATAAAT
TATTTGAGTGAAAATAATGGCATAAGAGAAATAGAAATGAAAGACATATTAGCGCGTTTT
ACCACGGATGTCATTGGCAGCGTGGCGTTTGGGATCCAAATTTCATCAATGCGCGACCCA
AAATCAATATTTAGAAAAATGGGAAGCAGAGTCTTCAATCCACCCAGATCACAAACATTA
AAAATCATTTTTCTTACAACATTTAGAAAATGGGCACGAAAATTTAGTTTCAGAGTTATT
GAGAAAGAAGTTGCTGACTTTTTTCTTTCAACGATTGAAGAAAACATCAATTATCGAGAG
AAAAACAATATTCAGAGAAATGATTTCTTTAATTTATTACTTGAATTAAAGAATCTTGGA
AAATTAAAAGACGACATAAGTGAAGCAGGAGAAAAATTGACATTTAATGAAATTGCAGCT
CAAAGTTTTCTATTCTATCTTGCTGGATTTG
>g51.t3 Gene=g51 Length=310
MSSLEVFLALFLVALGLCVFFIKKKFSYWKERGFDYIEPEFPFGNLKGVGYKIHFSELSR
EYYERYKNKTSAIGLYFFTQPVVFLTNLDAVKNILVKDFYNFHDRGLYVNTKSDPLSAHL
FAIEGAEWKRMRTKLSPTFTSMRMKTMFGTVLNISEEMINYLSENNGIREIEMKDILARF
TTDVIGSVAFGIQISSMRDPKSIFRKMGSRVFNPPRSQTLKIIFLTTFRKWARKFSFRVI
EKEVADFFLSTIEENINYREKNNIQRNDFFNLLLELKNLGKLKDDISEAGEKLTFNEIAA
QSFLFYLAGF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g51.t3 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 18 | 310 | 1.0E-56 |
| 2 | g51.t3 | PANTHER | PTHR24292 | CYTOCHROME P450 | 7 | 310 | 4.0E-99 |
| 3 | g51.t3 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 7 | 310 | 4.0E-99 |
| 4 | g51.t3 | PRINTS | PR00464 | Group II E-class P450 signature | 125 | 145 | 7.9E-10 |
| 5 | g51.t3 | PRINTS | PR00464 | Group II E-class P450 signature | 179 | 197 | 7.9E-10 |
| 1 | g51.t3 | Pfam | PF00067 | Cytochrome P450 | 43 | 310 | 1.9E-31 |
| 11 | g51.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 12 | g51.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 22 | - |
| 10 | g51.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 23 | 310 | - |
| 8 | g51.t3 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 44 | 310 | 4.19E-42 |
| 6 | g51.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 4 | 22 | - |
| 7 | g51.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 72 | 91 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g51/g51.t3; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g51.t3.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0004497 | monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed