General information for g5147.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as V-type proton ATPase subunit H.
Parent gene
- The parent gene g5147 is located on chr_2: 7283280..7284485 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5147 | g5147.t1 | TSS | g5147.t1 | 7281189 | 7281189 |
| chr_2 | g5147 | g5147.t1 | isoform | g5147.t1 | 7281299 | 7284128 |
| chr_2 | g5147 | g5147.t1 | exon | g5147.t1.exon1 | 7281299 | 7281350 |
| chr_2 | g5147 | g5147.t1 | cds | g5147.t1.CDS1 | 7281299 | 7281350 |
| chr_2 | g5147 | g5147.t1 | exon | g5147.t1.exon2 | 7281419 | 7281681 |
| chr_2 | g5147 | g5147.t1 | cds | g5147.t1.CDS2 | 7281419 | 7281681 |
| chr_2 | g5147 | g5147.t1 | exon | g5147.t1.exon3 | 7281740 | 7281958 |
| chr_2 | g5147 | g5147.t1 | cds | g5147.t1.CDS3 | 7281740 | 7281958 |
| chr_2 | g5147 | g5147.t1 | exon | g5147.t1.exon4 | 7283058 | 7283192 |
| chr_2 | g5147 | g5147.t1 | cds | g5147.t1.CDS4 | 7283058 | 7283192 |
| chr_2 | g5147 | g5147.t1 | exon | g5147.t1.exon5 | 7283250 | 7283812 |
| chr_2 | g5147 | g5147.t1 | cds | g5147.t1.CDS5 | 7283250 | 7283812 |
| chr_2 | g5147 | g5147.t1 | exon | g5147.t1.exon6 | 7283873 | 7283997 |
| chr_2 | g5147 | g5147.t1 | cds | g5147.t1.CDS6 | 7283873 | 7283997 |
| chr_2 | g5147 | g5147.t1 | exon | g5147.t1.exon7 | 7284052 | 7284128 |
| chr_2 | g5147 | g5147.t1 | cds | g5147.t1.CDS7 | 7284052 | 7284128 |
| chr_2 | g5147 | g5147.t1 | TTS | g5147.t1 | 7284480 | 7284480 |
Sequences
>g5147.t1 Gene=g5147 Length=1434
ATGGCCAGCAACGTCGATGACCTGATTTCATCTCATTTAGATGACAAAATTGATATGATT
GCTGCTACTAGTGTCTTACAGCAACAAGCTAGTGACATTCGAGCTCAGAAAATCAATTGG
CAATCATATCATCAATCTCAGATGATTTCGAATGAAGATTTTAATTGCATCACGGCTCTT
GATGGCGAGAAGAAAGCTCAATATTTGAAAGATAATCAGTATCAGAGCGCAAAAACCTTT
CTGAATCTTCTCAGTCATGTGTCGAAAGATACAACAATTCAATACTTGCTTGTCATGATT
GATGACTTACTTACTGAGGATCGAACTCGTGTCGATATATTTTTGGATTTTGCTTCTAAA
CGTAAAGAAACTGTTTGGGGACCTTTCCTTTCTTTATTGAATCGCTCTGATGGCTTCATC
ATTCATATGGCATCAAGAATTATCGCTAAATTGGCATGCTGGGGTCAAGATCAAATGCCC
AAATCAGACTTAAACTTCTACTTGCAATGGCTTAAAGATCAACTTACAGTCAGCGATAAT
GAATACATTCAATCAGTTGGACGTTGTTTGCAAATGATGCTCAGAGTTGATGAATATCGT
TTTGCCTTTGTTACAGTTGATGGCATCAGTACTTTGATTTCTATTCTTTCATCACGTGTT
AATTTCCAAGTGCAATATCAACTTGTATTTTGCTTATGGGTTTTGACTTTCAATCCTCTT
CTTGCTGAGAAGATGAATAAATTCAATGTTATTCCAATTCTTGCTGATATTCTTAGTGAT
TGTGCAAAGGAGAAAGTTACACGCATTATACTCGCCGTCTTTAGAAATTTGATCGAAAAG
CCAGAGGATGCACAAGTGTCGAAGGAACACTGCATTGCAATGGTTCAATGTAAAGTTCTC
AAACAATTGTCCATTCTCGAGCAACGTCGTTTTGACGATGAAGACATTACGTCTGATGTT
GAATTTCTAACAGAAAGATTGCAGACATCTGTTCAAGATTTGTCTTCATTTGATGAATAT
GCAACTGAAATCAAGAGCGGACGCTTAGAATGGTCACCTGTGCACAAATCAGCTAAATTC
TGGCGTGAAAATGCACAACGTTTGAATGAGAAGAATTATGAATTGCTTAGAATTTTAATT
CATTTACTGGACACATCACGCGATCCATTAGTTCTCTCAGTTGCCAGTTTTGACATTGGT
GAATATGTTCGTCATTATCCAAGAGGAAAGCATGTTATTGAACAATTGAATGGTAAACAA
TTGGTAATGCAACTTCTCGGACATGATGATCCCAATGTACGTTATGAGGCTCTTTTAGCT
GTTCAAAAATTAATGGTTCACAATTGGGAATACTTAGGAAAACAATTGGAAAAAGATAGT
GAAAAAGCACCTGGCGAGAAAGAAGGAGGCAAGGTGATTTCAGGAAAAGCTTAA
>g5147.t1 Gene=g5147 Length=477
MASNVDDLISSHLDDKIDMIAATSVLQQQASDIRAQKINWQSYHQSQMISNEDFNCITAL
DGEKKAQYLKDNQYQSAKTFLNLLSHVSKDTTIQYLLVMIDDLLTEDRTRVDIFLDFASK
RKETVWGPFLSLLNRSDGFIIHMASRIIAKLACWGQDQMPKSDLNFYLQWLKDQLTVSDN
EYIQSVGRCLQMMLRVDEYRFAFVTVDGISTLISILSSRVNFQVQYQLVFCLWVLTFNPL
LAEKMNKFNVIPILADILSDCAKEKVTRIILAVFRNLIEKPEDAQVSKEHCIAMVQCKVL
KQLSILEQRRFDDEDITSDVEFLTERLQTSVQDLSSFDEYATEIKSGRLEWSPVHKSAKF
WRENAQRLNEKNYELLRILIHLLDTSRDPLVLSVASFDIGEYVRHYPRGKHVIEQLNGKQ
LVMQLLGHDDPNVRYEALLAVQKLMVHNWEYLGKQLEKDSEKAPGEKEGGKVISGKA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g5147.t1 | CDD | cd00256 | VATPase_H | 24 | 450 | 0.0 |
| 6 | g5147.t1 | Gene3D | G3DSA:1.25.10.10 | - | 16 | 335 | 1.0E-51 |
| 7 | g5147.t1 | Gene3D | G3DSA:1.25.40.150 | - | 347 | 449 | 9.3E-38 |
| 18 | g5147.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 458 | 477 | - |
| 3 | g5147.t1 | PANTHER | PTHR10698:SF0 | V-TYPE PROTON ATPASE SUBUNIT H | 25 | 453 | 7.2E-159 |
| 4 | g5147.t1 | PANTHER | PTHR10698 | V-TYPE PROTON ATPASE SUBUNIT H | 25 | 453 | 7.2E-159 |
| 13 | g5147.t1 | PIRSF | PIRSF032184 | V-ATP_synth_H | 15 | 455 | 1.7E-127 |
| 2 | g5147.t1 | Pfam | PF03224 | V-ATPase subunit H | 24 | 327 | 1.0E-122 |
| 1 | g5147.t1 | Pfam | PF11698 | V-ATPase subunit H | 334 | 449 | 1.3E-45 |
| 9 | g5147.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 200 | - |
| 11 | g5147.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 201 | 218 | - |
| 10 | g5147.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 219 | 223 | - |
| 12 | g5147.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 224 | 242 | - |
| 8 | g5147.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 243 | 477 | - |
| 17 | g5147.t1 | SMART | SM00185 | arm_5 | 195 | 237 | 44.0 |
| 16 | g5147.t1 | SMART | SM00185 | arm_5 | 238 | 279 | 5.1 |
| 15 | g5147.t1 | SMART | SM00185 | arm_5 | 406 | 446 | 120.0 |
| 5 | g5147.t1 | SUPERFAMILY | SSF48371 | ARM repeat | 17 | 450 | 5.24E-147 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5147/g5147.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5147.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:1902600 | proton transmembrane transport | BP |
| GO:0005515 | protein binding | MF |
| GO:0000221 | vacuolar proton-transporting V-type ATPase, V1 domain | CC |
| GO:0046961 | proton-transporting ATPase activity, rotational mechanism | MF |
KEGG
Orthology
- This transcript has been annotated with K02144 (V-type H+-transporting ATPase subunit H)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
