General information for g5147.t11
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as V-type proton ATPase subunit H.
Parent gene
- The parent gene g5147 is located on chr_2: 7283280..7284485 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5147 | g5147.t11 | TSS | g5147.t11 | 7281189 | 7281189 |
| chr_2 | g5147 | g5147.t11 | isoform | g5147.t11 | 7281299 | 7284485 |
| chr_2 | g5147 | g5147.t11 | exon | g5147.t11.exon1 | 7281299 | 7281350 |
| chr_2 | g5147 | g5147.t11 | cds | g5147.t11.CDS1 | 7281299 | 7281350 |
| chr_2 | g5147 | g5147.t11 | exon | g5147.t11.exon2 | 7281419 | 7281681 |
| chr_2 | g5147 | g5147.t11 | cds | g5147.t11.CDS2 | 7281419 | 7281681 |
| chr_2 | g5147 | g5147.t11 | exon | g5147.t11.exon3 | 7281740 | 7281958 |
| chr_2 | g5147 | g5147.t11 | cds | g5147.t11.CDS3 | 7281740 | 7281958 |
| chr_2 | g5147 | g5147.t11 | exon | g5147.t11.exon4 | 7282573 | 7282851 |
| chr_2 | g5147 | g5147.t11 | cds | g5147.t11.CDS4 | 7282573 | 7282851 |
| chr_2 | g5147 | g5147.t11 | exon | g5147.t11.exon5 | 7283058 | 7283192 |
| chr_2 | g5147 | g5147.t11 | cds | g5147.t11.CDS5 | 7283058 | 7283192 |
| chr_2 | g5147 | g5147.t11 | exon | g5147.t11.exon6 | 7283250 | 7283812 |
| chr_2 | g5147 | g5147.t11 | cds | g5147.t11.CDS6 | 7283250 | 7283812 |
| chr_2 | g5147 | g5147.t11 | exon | g5147.t11.exon7 | 7283873 | 7283997 |
| chr_2 | g5147 | g5147.t11 | cds | g5147.t11.CDS7 | 7283873 | 7283997 |
| chr_2 | g5147 | g5147.t11 | exon | g5147.t11.exon8 | 7284052 | 7284485 |
| chr_2 | g5147 | g5147.t11 | cds | g5147.t11.CDS8 | 7284052 | 7284128 |
| chr_2 | g5147 | g5147.t11 | TTS | g5147.t11 | 7284480 | 7284480 |
Sequences
>g5147.t11 Gene=g5147 Length=2070
ATGGCCAGCAACGTCGATGACCTGATTTCATCTCATTTAGATGACAAAATTGATATGATT
GCTGCTACTAGTGTCTTACAGCAACAAGCTAGTGACATTCGAGCTCAGAAAATCAATTGG
CAATCATATCATCAATCTCAGATGATTTCGAATGAAGATTTTAATTGCATCACGGCTCTT
GATGGCGAGAAGAAAGCTCAATATTTGAAAGATAATCAGTATCAGAGCGCAAAAACCTTT
CTGAATCTTCTCAGTCATGTGTCGAAAGATACAACAATTCAATACTTGCTTGTCATGATT
GATGACTTACTTACTGAGGATCGAACTCGTGTCGATATATTTTTGGATTTTGCTTCTAAA
CGTAAAGAAACTGTTTGGGGACCTTTCCTTTCTTTATTGAATCGCTCTGATGGCTTCATC
ATTCATATGGCATCAAGAATTATCGCTAAATTGGCATGCTGGGGTCAAGATCAAATGCCC
AAATCAGACTTAAACTTCTACTTGCAATGGCTTAAAGATCAACTTACAGTCAGCGCGACT
GCATACAATAAGGAAATGGAAGAGATTCTTAAACATCGTGGATTTGAAAAGAAACATCAT
CATCATCACCATCATATGCATCTCTTCCACTTTCCAGGCCAACATGGCCATCATCATCAT
CACCAACACCATCACCACAACAAGGATCATCAGCAACAAACGGTGACTGTTGAGAAATAT
CGAGAGATTTCGAGCGCACTTGATGAGCCCTCGAATGCTGCACAAAACAGCTCTGCTTCA
CATTCGTCTACTTCTAATCATTTAAAAATGACGGATAATGAATACATTCAATCAGTTGGA
CGTTGTTTGCAAATGATGCTCAGAGTTGATGAATATCGTTTTGCCTTTGTTACAGTTGAT
GGCATCAGTACTTTGATTTCTATTCTTTCATCACGTGTTAATTTCCAAGTGCAATATCAA
CTTGTATTTTGCTTATGGGTTTTGACTTTCAATCCTCTTCTTGCTGAGAAGATGAATAAA
TTCAATGTTATTCCAATTCTTGCTGATATTCTTAGTGATTGTGCAAAGGAGAAAGTTACA
CGCATTATACTCGCCGTCTTTAGAAATTTGATCGAAAAGCCAGAGGATGCACAAGTGTCG
AAGGAACACTGCATTGCAATGGTTCAATGTAAAGTTCTCAAACAATTGTCCATTCTCGAG
CAACGTCGTTTTGACGATGAAGACATTACGTCTGATGTTGAATTTCTAACAGAAAGATTG
CAGACATCTGTTCAAGATTTGTCTTCATTTGATGAATATGCAACTGAAATCAAGAGCGGA
CGCTTAGAATGGTCACCTGTGCACAAATCAGCTAAATTCTGGCGTGAAAATGCACAACGT
TTGAATGAGAAGAATTATGAATTGCTTAGAATTTTAATTCATTTACTGGACACATCACGC
GATCCATTAGTTCTCTCAGTTGCCAGTTTTGACATTGGTGAATATGTTCGTCATTATCCA
AGAGGAAAGCATGTTATTGAACAATTGAATGGTAAACAATTGGTAATGCAACTTCTCGGA
CATGATGATCCCAATGTACGTTATGAGGCTCTTTTAGCTGTTCAAAAATTAATGGTTCAC
AATTGGGAATACTTAGGAAAACAATTGGAAAAAGATAGTGAAAAAGCACCTGGCGAGAAA
GAAGGAGGCAAGGTGATTTCAGGAAAAGCTTAAATATTTCTTATCACTTAAAACACTTGC
ATAAACAAGAAAAAATATACATCCATTAGTATTATTGAAAATAATCTATAATCAATGAAG
CAGAAGATATATCTTAATATGAATTATAAAATTATTATTGTTGATCAATTGTATGTACAT
AATGTTCCTCATTTCATAGTCAAAATTTTTGCTTGTTCTTTAACAATTTTACTTCTCTTA
AAATTTGGCTGAAAATTTAAGTCTTTATTTAGTCATTCCAAGACATTTCTTCATATATAA
TTTTGTGTTTAAAACTTATAAAAAGAAAAGTTTTGTTGTGCATCAATGAATAAATGAAAA
GAGAATAAAAATTTATGTGAATTGTAAAAA
>g5147.t11 Gene=g5147 Length=570
MASNVDDLISSHLDDKIDMIAATSVLQQQASDIRAQKINWQSYHQSQMISNEDFNCITAL
DGEKKAQYLKDNQYQSAKTFLNLLSHVSKDTTIQYLLVMIDDLLTEDRTRVDIFLDFASK
RKETVWGPFLSLLNRSDGFIIHMASRIIAKLACWGQDQMPKSDLNFYLQWLKDQLTVSAT
AYNKEMEEILKHRGFEKKHHHHHHHMHLFHFPGQHGHHHHHQHHHHNKDHQQQTVTVEKY
REISSALDEPSNAAQNSSASHSSTSNHLKMTDNEYIQSVGRCLQMMLRVDEYRFAFVTVD
GISTLISILSSRVNFQVQYQLVFCLWVLTFNPLLAEKMNKFNVIPILADILSDCAKEKVT
RIILAVFRNLIEKPEDAQVSKEHCIAMVQCKVLKQLSILEQRRFDDEDITSDVEFLTERL
QTSVQDLSSFDEYATEIKSGRLEWSPVHKSAKFWRENAQRLNEKNYELLRILIHLLDTSR
DPLVLSVASFDIGEYVRHYPRGKHVIEQLNGKQLVMQLLGHDDPNVRYEALLAVQKLMVH
NWEYLGKQLEKDSEKAPGEKEGGKVISGKA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g5147.t11 | CDD | cd00256 | VATPase_H | 24 | 543 | 0.0 |
| 7 | g5147.t11 | Gene3D | G3DSA:1.25.10.10 | - | 16 | 190 | 7.3E-11 |
| 8 | g5147.t11 | Gene3D | G3DSA:1.25.10.10 | - | 191 | 428 | 4.0E-39 |
| 9 | g5147.t11 | Gene3D | G3DSA:1.25.40.150 | - | 440 | 542 | 1.2E-37 |
| 21 | g5147.t11 | MobiDBLite | mobidb-lite | consensus disorder prediction | 246 | 266 | - |
| 19 | g5147.t11 | MobiDBLite | mobidb-lite | consensus disorder prediction | 248 | 266 | - |
| 20 | g5147.t11 | MobiDBLite | mobidb-lite | consensus disorder prediction | 551 | 570 | - |
| 3 | g5147.t11 | PANTHER | PTHR10698:SF0 | V-TYPE PROTON ATPASE SUBUNIT H | 25 | 546 | 1.9E-154 |
| 4 | g5147.t11 | PANTHER | PTHR10698 | V-TYPE PROTON ATPASE SUBUNIT H | 25 | 546 | 1.9E-154 |
| 2 | g5147.t11 | Pfam | PF03224 | V-ATPase subunit H | 24 | 420 | 3.6E-118 |
| 1 | g5147.t11 | Pfam | PF11698 | V-ATPase subunit H | 427 | 542 | 1.8E-45 |
| 11 | g5147.t11 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 293 | - |
| 13 | g5147.t11 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 294 | 311 | - |
| 12 | g5147.t11 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 312 | 316 | - |
| 14 | g5147.t11 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 317 | 335 | - |
| 10 | g5147.t11 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 336 | 570 | - |
| 17 | g5147.t11 | SMART | SM00185 | arm_5 | 288 | 330 | 44.0 |
| 18 | g5147.t11 | SMART | SM00185 | arm_5 | 331 | 372 | 5.1 |
| 16 | g5147.t11 | SMART | SM00185 | arm_5 | 499 | 539 | 120.0 |
| 6 | g5147.t11 | SUPERFAMILY | SSF48371 | ARM repeat | 17 | 221 | 8.25E-32 |
| 5 | g5147.t11 | SUPERFAMILY | SSF48371 | ARM repeat | 217 | 543 | 6.06E-112 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5147/g5147.t11; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5147.t11.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:1902600 | proton transmembrane transport | BP |
| GO:0005515 | protein binding | MF |
| GO:0000221 | vacuolar proton-transporting V-type ATPase, V1 domain | CC |
| GO:0046961 | proton-transporting ATPase activity, rotational mechanism | MF |
KEGG
Orthology
- This transcript has been annotated with K02144 (V-type H+-transporting ATPase subunit H)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
