General information for g5147.t12
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as V-type proton ATPase subunit H.
Parent gene
- The parent gene g5147 is located on chr_2: 7283280..7284485 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5147 | g5147.t12 | TSS | g5147.t12 | 7281189 | 7281189 |
| chr_2 | g5147 | g5147.t12 | isoform | g5147.t12 | 7281299 | 7284485 |
| chr_2 | g5147 | g5147.t12 | exon | g5147.t12.exon1 | 7281299 | 7281681 |
| chr_2 | g5147 | g5147.t12 | cds | g5147.t12.CDS1 | 7281421 | 7281681 |
| chr_2 | g5147 | g5147.t12 | exon | g5147.t12.exon2 | 7281740 | 7281958 |
| chr_2 | g5147 | g5147.t12 | cds | g5147.t12.CDS2 | 7281740 | 7281958 |
| chr_2 | g5147 | g5147.t12 | exon | g5147.t12.exon3 | 7283058 | 7283192 |
| chr_2 | g5147 | g5147.t12 | cds | g5147.t12.CDS3 | 7283058 | 7283192 |
| chr_2 | g5147 | g5147.t12 | exon | g5147.t12.exon4 | 7283250 | 7283812 |
| chr_2 | g5147 | g5147.t12 | cds | g5147.t12.CDS4 | 7283250 | 7283812 |
| chr_2 | g5147 | g5147.t12 | exon | g5147.t12.exon5 | 7283873 | 7283997 |
| chr_2 | g5147 | g5147.t12 | cds | g5147.t12.CDS5 | 7283873 | 7283997 |
| chr_2 | g5147 | g5147.t12 | exon | g5147.t12.exon6 | 7284052 | 7284485 |
| chr_2 | g5147 | g5147.t12 | cds | g5147.t12.CDS6 | 7284052 | 7284128 |
| chr_2 | g5147 | g5147.t12 | TTS | g5147.t12 | 7284480 | 7284480 |
Sequences
>g5147.t12 Gene=g5147 Length=1859
ATGGCCAGCAACGTCGATGACCTGATTTCATCTCATTTAGATGACAAAATTGGTAAGAAA
ACTATTGTTTTGATCAATATTTCTTCAGTTTTCAATAATTTTTTTTTCAACAACATCCAG
ATATGATTGCTGCTACTAGTGTCTTACAGCAACAAGCTAGTGACATTCGAGCTCAGAAAA
TCAATTGGCAATCATATCATCAATCTCAGATGATTTCGAATGAAGATTTTAATTGCATCA
CGGCTCTTGATGGCGAGAAGAAAGCTCAATATTTGAAAGATAATCAGTATCAGAGCGCAA
AAACCTTTCTGAATCTTCTCAGTCATGTGTCGAAAGATACAACAATTCAATACTTGCTTG
TCATGATTGATGACTTACTTACTGAGGATCGAACTCGTGTCGATATATTTTTGGATTTTG
CTTCTAAACGTAAAGAAACTGTTTGGGGACCTTTCCTTTCTTTATTGAATCGCTCTGATG
GCTTCATCATTCATATGGCATCAAGAATTATCGCTAAATTGGCATGCTGGGGTCAAGATC
AAATGCCCAAATCAGACTTAAACTTCTACTTGCAATGGCTTAAAGATCAACTTACAGTCA
GCGATAATGAATACATTCAATCAGTTGGACGTTGTTTGCAAATGATGCTCAGAGTTGATG
AATATCGTTTTGCCTTTGTTACAGTTGATGGCATCAGTACTTTGATTTCTATTCTTTCAT
CACGTGTTAATTTCCAAGTGCAATATCAACTTGTATTTTGCTTATGGGTTTTGACTTTCA
ATCCTCTTCTTGCTGAGAAGATGAATAAATTCAATGTTATTCCAATTCTTGCTGATATTC
TTAGTGATTGTGCAAAGGAGAAAGTTACACGCATTATACTCGCCGTCTTTAGAAATTTGA
TCGAAAAGCCAGAGGATGCACAAGTGTCGAAGGAACACTGCATTGCAATGGTTCAATGTA
AAGTTCTCAAACAATTGTCCATTCTCGAGCAACGTCGTTTTGACGATGAAGACATTACGT
CTGATGTTGAATTTCTAACAGAAAGATTGCAGACATCTGTTCAAGATTTGTCTTCATTTG
ATGAATATGCAACTGAAATCAAGAGCGGACGCTTAGAATGGTCACCTGTGCACAAATCAG
CTAAATTCTGGCGTGAAAATGCACAACGTTTGAATGAGAAGAATTATGAATTGCTTAGAA
TTTTAATTCATTTACTGGACACATCACGCGATCCATTAGTTCTCTCAGTTGCCAGTTTTG
ACATTGGTGAATATGTTCGTCATTATCCAAGAGGAAAGCATGTTATTGAACAATTGAATG
GTAAACAATTGGTAATGCAACTTCTCGGACATGATGATCCCAATGTACGTTATGAGGCTC
TTTTAGCTGTTCAAAAATTAATGGTTCACAATTGGGAATACTTAGGAAAACAATTGGAAA
AAGATAGTGAAAAAGCACCTGGCGAGAAAGAAGGAGGCAAGGTGATTTCAGGAAAAGCTT
AAATATTTCTTATCACTTAAAACACTTGCATAAACAAGAAAAAATATACATCCATTAGTA
TTATTGAAAATAATCTATAATCAATGAAGCAGAAGATATATCTTAATATGAATTATAAAA
TTATTATTGTTGATCAATTGTATGTACATAATGTTCCTCATTTCATAGTCAAAATTTTTG
CTTGTTCTTTAACAATTTTACTTCTCTTAAAATTTGGCTGAAAATTTAAGTCTTTATTTA
GTCATTCCAAGACATTTCTTCATATATAATTTTGTGTTTAAAACTTATAAAAAGAAAAGT
TTTGTTGTGCATCAATGAATAAATGAAAAGAGAATAAAAATTTATGTGAATTGTAAAAA
>g5147.t12 Gene=g5147 Length=459
MIAATSVLQQQASDIRAQKINWQSYHQSQMISNEDFNCITALDGEKKAQYLKDNQYQSAK
TFLNLLSHVSKDTTIQYLLVMIDDLLTEDRTRVDIFLDFASKRKETVWGPFLSLLNRSDG
FIIHMASRIIAKLACWGQDQMPKSDLNFYLQWLKDQLTVSDNEYIQSVGRCLQMMLRVDE
YRFAFVTVDGISTLISILSSRVNFQVQYQLVFCLWVLTFNPLLAEKMNKFNVIPILADIL
SDCAKEKVTRIILAVFRNLIEKPEDAQVSKEHCIAMVQCKVLKQLSILEQRRFDDEDITS
DVEFLTERLQTSVQDLSSFDEYATEIKSGRLEWSPVHKSAKFWRENAQRLNEKNYELLRI
LIHLLDTSRDPLVLSVASFDIGEYVRHYPRGKHVIEQLNGKQLVMQLLGHDDPNVRYEAL
LAVQKLMVHNWEYLGKQLEKDSEKAPGEKEGGKVISGKA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g5147.t12 | CDD | cd00256 | VATPase_H | 6 | 432 | 0.0 |
| 6 | g5147.t12 | Gene3D | G3DSA:1.25.10.10 | - | 1 | 317 | 8.2E-52 |
| 7 | g5147.t12 | Gene3D | G3DSA:1.25.40.150 | - | 329 | 431 | 8.7E-38 |
| 18 | g5147.t12 | MobiDBLite | mobidb-lite | consensus disorder prediction | 440 | 459 | - |
| 3 | g5147.t12 | PANTHER | PTHR10698:SF0 | V-TYPE PROTON ATPASE SUBUNIT H | 7 | 435 | 6.5E-159 |
| 4 | g5147.t12 | PANTHER | PTHR10698 | V-TYPE PROTON ATPASE SUBUNIT H | 7 | 435 | 6.5E-159 |
| 13 | g5147.t12 | PIRSF | PIRSF032184 | V-ATP_synth_H | 1 | 437 | 1.9E-127 |
| 2 | g5147.t12 | Pfam | PF03224 | V-ATPase subunit H | 6 | 309 | 9.3E-123 |
| 1 | g5147.t12 | Pfam | PF11698 | V-ATPase subunit H | 316 | 431 | 1.2E-45 |
| 8 | g5147.t12 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 182 | - |
| 12 | g5147.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 183 | 200 | - |
| 10 | g5147.t12 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 201 | 205 | - |
| 11 | g5147.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 206 | 224 | - |
| 9 | g5147.t12 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 225 | 459 | - |
| 17 | g5147.t12 | SMART | SM00185 | arm_5 | 177 | 219 | 44.0 |
| 16 | g5147.t12 | SMART | SM00185 | arm_5 | 220 | 261 | 5.1 |
| 15 | g5147.t12 | SMART | SM00185 | arm_5 | 388 | 428 | 120.0 |
| 5 | g5147.t12 | SUPERFAMILY | SSF48371 | ARM repeat | 2 | 432 | 1.89E-146 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5147/g5147.t12; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5147.t12.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:1902600 | proton transmembrane transport | BP |
| GO:0005515 | protein binding | MF |
| GO:0000221 | vacuolar proton-transporting V-type ATPase, V1 domain | CC |
| GO:0046961 | proton-transporting ATPase activity, rotational mechanism | MF |
KEGG
Orthology
- This transcript has been annotated with K02144 (V-type H+-transporting ATPase subunit H)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
