General information for g5147.t16
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as V-type proton ATPase subunit H.
Parent gene
- The parent gene g5147 is located on chr_2: 7283280..7284485 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5147 | g5147.t16 | isoform | g5147.t16 | 7283070 | 7284128 |
| chr_2 | g5147 | g5147.t16 | exon | g5147.t16.exon1 | 7283070 | 7283192 |
| chr_2 | g5147 | g5147.t16 | cds | g5147.t16.CDS1 | 7283097 | 7283192 |
| chr_2 | g5147 | g5147.t16 | exon | g5147.t16.exon2 | 7283250 | 7283812 |
| chr_2 | g5147 | g5147.t16 | cds | g5147.t16.CDS2 | 7283250 | 7283812 |
| chr_2 | g5147 | g5147.t16 | exon | g5147.t16.exon3 | 7283873 | 7283997 |
| chr_2 | g5147 | g5147.t16 | cds | g5147.t16.CDS3 | 7283873 | 7283997 |
| chr_2 | g5147 | g5147.t16 | exon | g5147.t16.exon4 | 7284052 | 7284128 |
| chr_2 | g5147 | g5147.t16 | cds | g5147.t16.CDS4 | 7284052 | 7284128 |
| chr_2 | g5147 | g5147.t16 | TTS | g5147.t16 | 7284480 | 7284480 |
| chr_2 | g5147 | g5147.t16 | TSS | g5147.t16 | NA | NA |
Sequences
>g5147.t16 Gene=g5147 Length=888
ATTCAATCAGTTGGACGTTGTTTGCAAATGATGCTCAGAGTTGATGAATATCGTTTTGCC
TTTGTTACAGTTGATGGCATCAGTACTTTGATTTCTATTCTTTCATCACGTGTTAATTTC
CAAGTGCAATATCAACTTGTATTTTGCTTATGGGTTTTGACTTTCAATCCTCTTCTTGCT
GAGAAGATGAATAAATTCAATGTTATTCCAATTCTTGCTGATATTCTTAGTGATTGTGCA
AAGGAGAAAGTTACACGCATTATACTCGCCGTCTTTAGAAATTTGATCGAAAAGCCAGAG
GATGCACAAGTGTCGAAGGAACACTGCATTGCAATGGTTCAATGTAAAGTTCTCAAACAA
TTGTCCATTCTCGAGCAACGTCGTTTTGACGATGAAGACATTACGTCTGATGTTGAATTT
CTAACAGAAAGATTGCAGACATCTGTTCAAGATTTGTCTTCATTTGATGAATATGCAACT
GAAATCAAGAGCGGACGCTTAGAATGGTCACCTGTGCACAAATCAGCTAAATTCTGGCGT
GAAAATGCACAACGTTTGAATGAGAAGAATTATGAATTGCTTAGAATTTTAATTCATTTA
CTGGACACATCACGCGATCCATTAGTTCTCTCAGTTGCCAGTTTTGACATTGGTGAATAT
GTTCGTCATTATCCAAGAGGAAAGCATGTTATTGAACAATTGAATGGTAAACAATTGGTA
ATGCAACTTCTCGGACATGATGATCCCAATGTACGTTATGAGGCTCTTTTAGCTGTTCAA
AAATTAATGGTTCACAATTGGGAATACTTAGGAAAACAATTGGAAAAAGATAGTGAAAAA
GCACCTGGCGAGAAAGAAGGAGGCAAGGTGATTTCAGGAAAAGCTTAA
>g5147.t16 Gene=g5147 Length=286
MMLRVDEYRFAFVTVDGISTLISILSSRVNFQVQYQLVFCLWVLTFNPLLAEKMNKFNVI
PILADILSDCAKEKVTRIILAVFRNLIEKPEDAQVSKEHCIAMVQCKVLKQLSILEQRRF
DDEDITSDVEFLTERLQTSVQDLSSFDEYATEIKSGRLEWSPVHKSAKFWRENAQRLNEK
NYELLRILIHLLDTSRDPLVLSVASFDIGEYVRHYPRGKHVIEQLNGKQLVMQLLGHDDP
NVRYEALLAVQKLMVHNWEYLGKQLEKDSEKAPGEKEGGKVISGKA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g5147.t16 | Gene3D | G3DSA:1.25.10.10 | - | 7 | 144 | 1.3E-37 |
| 11 | g5147.t16 | Gene3D | G3DSA:1.25.40.150 | - | 156 | 258 | 3.6E-38 |
| 9 | g5147.t16 | MobiDBLite | mobidb-lite | consensus disorder prediction | 267 | 286 | - |
| 3 | g5147.t16 | PANTHER | PTHR10698:SF0 | V-TYPE PROTON ATPASE SUBUNIT H | 1 | 262 | 4.1E-110 |
| 4 | g5147.t16 | PANTHER | PTHR10698 | V-TYPE PROTON ATPASE SUBUNIT H | 1 | 262 | 4.1E-110 |
| 2 | g5147.t16 | Pfam | PF03224 | V-ATPase subunit H | 1 | 136 | 2.3E-59 |
| 1 | g5147.t16 | Pfam | PF11698 | V-ATPase subunit H | 143 | 258 | 4.8E-46 |
| 12 | g5147.t16 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 6 | - |
| 16 | g5147.t16 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 27 | - |
| 14 | g5147.t16 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 28 | 32 | - |
| 15 | g5147.t16 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 33 | 51 | - |
| 13 | g5147.t16 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 52 | 286 | - |
| 6 | g5147.t16 | SMART | SM00185 | arm_5 | 4 | 46 | 44.0 |
| 7 | g5147.t16 | SMART | SM00185 | arm_5 | 47 | 88 | 5.1 |
| 8 | g5147.t16 | SMART | SM00185 | arm_5 | 215 | 255 | 120.0 |
| 5 | g5147.t16 | SUPERFAMILY | SSF48371 | ARM repeat | 2 | 259 | 1.66E-108 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5147/g5147.t16; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5147.t16.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:1902600 | proton transmembrane transport | BP |
| GO:0005515 | protein binding | MF |
| GO:0000221 | vacuolar proton-transporting V-type ATPase, V1 domain | CC |
| GO:0046961 | proton-transporting ATPase activity, rotational mechanism | MF |
KEGG
Orthology
- This transcript has been annotated with K02144 (V-type H+-transporting ATPase subunit H)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
