General information for g5147.t9
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as V-type proton ATPase subunit H.
Parent gene
- The parent gene g5147 is located on chr_2: 7283280..7284485 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5147 | g5147.t9 | TSS | g5147.t9 | 7281189 | 7281189 |
| chr_2 | g5147 | g5147.t9 | isoform | g5147.t9 | 7281299 | 7284485 |
| chr_2 | g5147 | g5147.t9 | exon | g5147.t9.exon1 | 7281299 | 7281350 |
| chr_2 | g5147 | g5147.t9 | cds | g5147.t9.CDS1 | 7281299 | 7281350 |
| chr_2 | g5147 | g5147.t9 | exon | g5147.t9.exon2 | 7281419 | 7281660 |
| chr_2 | g5147 | g5147.t9 | cds | g5147.t9.CDS2 | 7281419 | 7281660 |
| chr_2 | g5147 | g5147.t9 | exon | g5147.t9.exon3 | 7281740 | 7281958 |
| chr_2 | g5147 | g5147.t9 | cds | g5147.t9.CDS3 | 7281740 | 7281958 |
| chr_2 | g5147 | g5147.t9 | exon | g5147.t9.exon4 | 7283058 | 7283192 |
| chr_2 | g5147 | g5147.t9 | cds | g5147.t9.CDS4 | 7283058 | 7283192 |
| chr_2 | g5147 | g5147.t9 | exon | g5147.t9.exon5 | 7283250 | 7283812 |
| chr_2 | g5147 | g5147.t9 | cds | g5147.t9.CDS5 | 7283250 | 7283812 |
| chr_2 | g5147 | g5147.t9 | exon | g5147.t9.exon6 | 7283873 | 7283997 |
| chr_2 | g5147 | g5147.t9 | cds | g5147.t9.CDS6 | 7283873 | 7283997 |
| chr_2 | g5147 | g5147.t9 | exon | g5147.t9.exon7 | 7284052 | 7284485 |
| chr_2 | g5147 | g5147.t9 | cds | g5147.t9.CDS7 | 7284052 | 7284128 |
| chr_2 | g5147 | g5147.t9 | TTS | g5147.t9 | 7284480 | 7284480 |
Sequences
>g5147.t9 Gene=g5147 Length=1770
ATGGCCAGCAACGTCGATGACCTGATTTCATCTCATTTAGATGACAAAATTGATATGATT
GCTGCTACTAGTGTCTTACAGCAACAAGCTAGTGACATTCGAGCTCAGAAAATCAATTGG
CAATCATATCATCAATCTCAGATGATTTCGAATGAAGATTTTAATTGCATCACGGCTCTT
GATGGCGAGAAGAAAGCTCAATATTTGAAAGATAATCAGTATCAGAGCGCAAAAACCTTT
CTGAATCTTCTCAGTCATGTGTCGAAAGATACAACAATTCAATACTTGCTTGTCGAGGAT
CGAACTCGTGTCGATATATTTTTGGATTTTGCTTCTAAACGTAAAGAAACTGTTTGGGGA
CCTTTCCTTTCTTTATTGAATCGCTCTGATGGCTTCATCATTCATATGGCATCAAGAATT
ATCGCTAAATTGGCATGCTGGGGTCAAGATCAAATGCCCAAATCAGACTTAAACTTCTAC
TTGCAATGGCTTAAAGATCAACTTACAGTCAGCGATAATGAATACATTCAATCAGTTGGA
CGTTGTTTGCAAATGATGCTCAGAGTTGATGAATATCGTTTTGCCTTTGTTACAGTTGAT
GGCATCAGTACTTTGATTTCTATTCTTTCATCACGTGTTAATTTCCAAGTGCAATATCAA
CTTGTATTTTGCTTATGGGTTTTGACTTTCAATCCTCTTCTTGCTGAGAAGATGAATAAA
TTCAATGTTATTCCAATTCTTGCTGATATTCTTAGTGATTGTGCAAAGGAGAAAGTTACA
CGCATTATACTCGCCGTCTTTAGAAATTTGATCGAAAAGCCAGAGGATGCACAAGTGTCG
AAGGAACACTGCATTGCAATGGTTCAATGTAAAGTTCTCAAACAATTGTCCATTCTCGAG
CAACGTCGTTTTGACGATGAAGACATTACGTCTGATGTTGAATTTCTAACAGAAAGATTG
CAGACATCTGTTCAAGATTTGTCTTCATTTGATGAATATGCAACTGAAATCAAGAGCGGA
CGCTTAGAATGGTCACCTGTGCACAAATCAGCTAAATTCTGGCGTGAAAATGCACAACGT
TTGAATGAGAAGAATTATGAATTGCTTAGAATTTTAATTCATTTACTGGACACATCACGC
GATCCATTAGTTCTCTCAGTTGCCAGTTTTGACATTGGTGAATATGTTCGTCATTATCCA
AGAGGAAAGCATGTTATTGAACAATTGAATGGTAAACAATTGGTAATGCAACTTCTCGGA
CATGATGATCCCAATGTACGTTATGAGGCTCTTTTAGCTGTTCAAAAATTAATGGTTCAC
AATTGGGAATACTTAGGAAAACAATTGGAAAAAGATAGTGAAAAAGCACCTGGCGAGAAA
GAAGGAGGCAAGGTGATTTCAGGAAAAGCTTAAATATTTCTTATCACTTAAAACACTTGC
ATAAACAAGAAAAAATATACATCCATTAGTATTATTGAAAATAATCTATAATCAATGAAG
CAGAAGATATATCTTAATATGAATTATAAAATTATTATTGTTGATCAATTGTATGTACAT
AATGTTCCTCATTTCATAGTCAAAATTTTTGCTTGTTCTTTAACAATTTTACTTCTCTTA
AAATTTGGCTGAAAATTTAAGTCTTTATTTAGTCATTCCAAGACATTTCTTCATATATAA
TTTTGTGTTTAAAACTTATAAAAAGAAAAGTTTTGTTGTGCATCAATGAATAAATGAAAA
GAGAATAAAAATTTATGTGAATTGTAAAAA
>g5147.t9 Gene=g5147 Length=470
MASNVDDLISSHLDDKIDMIAATSVLQQQASDIRAQKINWQSYHQSQMISNEDFNCITAL
DGEKKAQYLKDNQYQSAKTFLNLLSHVSKDTTIQYLLVEDRTRVDIFLDFASKRKETVWG
PFLSLLNRSDGFIIHMASRIIAKLACWGQDQMPKSDLNFYLQWLKDQLTVSDNEYIQSVG
RCLQMMLRVDEYRFAFVTVDGISTLISILSSRVNFQVQYQLVFCLWVLTFNPLLAEKMNK
FNVIPILADILSDCAKEKVTRIILAVFRNLIEKPEDAQVSKEHCIAMVQCKVLKQLSILE
QRRFDDEDITSDVEFLTERLQTSVQDLSSFDEYATEIKSGRLEWSPVHKSAKFWRENAQR
LNEKNYELLRILIHLLDTSRDPLVLSVASFDIGEYVRHYPRGKHVIEQLNGKQLVMQLLG
HDDPNVRYEALLAVQKLMVHNWEYLGKQLEKDSEKAPGEKEGGKVISGKA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g5147.t9 | CDD | cd00256 | VATPase_H | 24 | 443 | 0.0 |
| 6 | g5147.t9 | Gene3D | G3DSA:1.25.10.10 | - | 16 | 328 | 4.1E-46 |
| 7 | g5147.t9 | Gene3D | G3DSA:1.25.40.150 | - | 340 | 442 | 9.1E-38 |
| 18 | g5147.t9 | MobiDBLite | mobidb-lite | consensus disorder prediction | 451 | 470 | - |
| 3 | g5147.t9 | PANTHER | PTHR10698:SF0 | V-TYPE PROTON ATPASE SUBUNIT H | 25 | 446 | 5.1E-152 |
| 4 | g5147.t9 | PANTHER | PTHR10698 | V-TYPE PROTON ATPASE SUBUNIT H | 25 | 446 | 5.1E-152 |
| 13 | g5147.t9 | PIRSF | PIRSF032184 | V-ATP_synth_H | 15 | 448 | 2.2E-119 |
| 2 | g5147.t9 | Pfam | PF03224 | V-ATPase subunit H | 24 | 320 | 1.6E-114 |
| 1 | g5147.t9 | Pfam | PF11698 | V-ATPase subunit H | 327 | 442 | 1.3E-45 |
| 9 | g5147.t9 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 193 | - |
| 11 | g5147.t9 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 194 | 211 | - |
| 10 | g5147.t9 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 212 | 216 | - |
| 12 | g5147.t9 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 217 | 235 | - |
| 8 | g5147.t9 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 236 | 470 | - |
| 15 | g5147.t9 | SMART | SM00185 | arm_5 | 188 | 230 | 44.0 |
| 17 | g5147.t9 | SMART | SM00185 | arm_5 | 231 | 272 | 5.1 |
| 16 | g5147.t9 | SMART | SM00185 | arm_5 | 399 | 439 | 120.0 |
| 5 | g5147.t9 | SUPERFAMILY | SSF48371 | ARM repeat | 17 | 443 | 4.03E-141 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5147/g5147.t9; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5147.t9.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:1902600 | proton transmembrane transport | BP |
| GO:0005515 | protein binding | MF |
| GO:0000221 | vacuolar proton-transporting V-type ATPase, V1 domain | CC |
| GO:0046961 | proton-transporting ATPase activity, rotational mechanism | MF |
KEGG
Orthology
- This transcript has been annotated with K02144 (V-type H+-transporting ATPase subunit H)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed