General information for g5177.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dopamine receptor 1.
Parent gene
- The parent gene g5177 is located on chr_2: 7514759..7532022 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5177 | g5177.t1 | TTS | g5177.t1 | 7514667 | 7514667 |
| chr_2 | g5177 | g5177.t1 | isoform | g5177.t1 | 7514759 | 7532022 |
| chr_2 | g5177 | g5177.t1 | exon | g5177.t1.exon1 | 7514759 | 7515078 |
| chr_2 | g5177 | g5177.t1 | cds | g5177.t1.CDS1 | 7514759 | 7515078 |
| chr_2 | g5177 | g5177.t1 | exon | g5177.t1.exon2 | 7517508 | 7517719 |
| chr_2 | g5177 | g5177.t1 | cds | g5177.t1.CDS2 | 7517508 | 7517719 |
| chr_2 | g5177 | g5177.t1 | exon | g5177.t1.exon3 | 7517802 | 7518047 |
| chr_2 | g5177 | g5177.t1 | cds | g5177.t1.CDS3 | 7517802 | 7518047 |
| chr_2 | g5177 | g5177.t1 | exon | g5177.t1.exon4 | 7518544 | 7518673 |
| chr_2 | g5177 | g5177.t1 | cds | g5177.t1.CDS4 | 7518544 | 7518673 |
| chr_2 | g5177 | g5177.t1 | exon | g5177.t1.exon5 | 7519010 | 7519189 |
| chr_2 | g5177 | g5177.t1 | cds | g5177.t1.CDS5 | 7519010 | 7519189 |
| chr_2 | g5177 | g5177.t1 | exon | g5177.t1.exon6 | 7519703 | 7519829 |
| chr_2 | g5177 | g5177.t1 | cds | g5177.t1.CDS6 | 7519703 | 7519829 |
| chr_2 | g5177 | g5177.t1 | exon | g5177.t1.exon7 | 7531966 | 7532022 |
| chr_2 | g5177 | g5177.t1 | cds | g5177.t1.CDS7 | 7531966 | 7532022 |
| chr_2 | g5177 | g5177.t1 | TSS | g5177.t1 | NA | NA |
Sequences
>g5177.t1 Gene=g5177 Length=1272
ATGGATGTGACCGACGAGGATATAGAGAATAAATGGTTGGAGCTTTATGAAAGCACGACT
TCCTTCTATTTCACTAACGACTCGTGGCCAAATGCCGAGGTCCTACCAGCCAATTTTGAT
GATTTGGATATCGTAGTTTCAGATGAAGGCTTAGGATTATCGTCATGGCGATTAGTGGTT
GTAGGAATATTTCTAGTGTTTCTAGTCTTCCTATCTATTGCGGGAAATATTCTCGTGTGT
ATTGCTATCTATACAGAACGAAGTCTTCGACGTATTGGCAATTTATTTCTGGCATCACTT
GCCATTGCCGATTTATTTGTAGCGTCTCTTGTGATGACATTCGCTGGCGTGAATGACATT
TTAGGTTATTGGATTTTTGGTGCTAAATTTTGCGATATATGGATAGCGTTTGATGTCATG
TGCTCAACTGCATCAATTTTAAATTTATGTGCCATTTCATTGGATCGATATATTCACATA
AAAGATCCATTAAGATATGGCCGTTGGGTAACGAGGCGTGTTACAGTAACAACTATAGCT
GTAATATGGTCGCTAGCCGCTTTCGTATCTTTTGTGCCAATTTCTTTCAATTTACATCGT
CCTGATACACCATTAATGTATGATAAGGACGGAACTGAATATGCGACGTGCGCACTTGAC
TTAACGCCAACCTATGCAATTGTATCGAGTTGTATAAGCTTTTATGTTCCATGTATAGTA
ATGATAGCAATTTATTGTAGACTTTATTGTTATGCCCAAAAGCATGTGAAATCGATTAAG
GCAGTAACAAAGGATCCCTGTGAAATCACAGAGAAGAGATACCGGAGCATTAGGCGCCTT
AATAAGCCAAATAAGAAGCTGAAAATACGCAGCCTTGTACATTCGTCACCATATCACGTT
TCCGATCACAAAGCAGCAGTTACTGTGGGCGTCATAATGGGCGTTTTTCTTGTATGTTGG
GTTCCATTTTTCTGTGTCAACATAGTGGCAGCATTCTGTAAGACATGCATCGACGGTCAA
GCCTTCAAAATTCTTTCGTGGCTGGGTTACAGTAACAGCAGTTTCAATCCAATAATTTAC
TCCATATTTAACAAGGAATTTCGTGATGCTTTCAAACGAATTTTAACATCAAAATCACAA
TGTTGGACAGAAGATCTCAATTATCATTGCCGTAATTCTGAACGATTTGTGACGGACTAT
CAATCAGCAAAATGTTCGCATGTTCATTCTGGTCGATCATCCGCTGATATTGAGCAAGTT
TCAGCTATCTAG
>g5177.t1 Gene=g5177 Length=423
MDVTDEDIENKWLELYESTTSFYFTNDSWPNAEVLPANFDDLDIVVSDEGLGLSSWRLVV
VGIFLVFLVFLSIAGNILVCIAIYTERSLRRIGNLFLASLAIADLFVASLVMTFAGVNDI
LGYWIFGAKFCDIWIAFDVMCSTASILNLCAISLDRYIHIKDPLRYGRWVTRRVTVTTIA
VIWSLAAFVSFVPISFNLHRPDTPLMYDKDGTEYATCALDLTPTYAIVSSCISFYVPCIV
MIAIYCRLYCYAQKHVKSIKAVTKDPCEITEKRYRSIRRLNKPNKKLKIRSLVHSSPYHV
SDHKAAVTVGVIMGVFLVCWVPFFCVNIVAAFCKTCIDGQAFKILSWLGYSNSSFNPIIY
SIFNKEFRDAFKRILTSKSQCWTEDLNYHCRNSERFVTDYQSAKCSHVHSGRSSADIEQV
SAI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 32 | g5177.t1 | CDD | cd15065 | 7tmA_Ap5-HTB1-like | 60 | 371 | 1.37299E-164 |
| 16 | g5177.t1 | Gene3D | G3DSA:1.20.1070.10 | - | 52 | 389 | 5.0E-89 |
| 2 | g5177.t1 | PANTHER | PTHR24247:SF238 | DOPAMINE RECEPTOR 1 | 50 | 398 | 5.6E-119 |
| 3 | g5177.t1 | PANTHER | PTHR24247 | 5-HYDROXYTRYPTAMINE RECEPTOR | 50 | 398 | 5.6E-119 |
| 5 | g5177.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 60 | 84 | 1.4E-47 |
| 12 | g5177.t1 | PRINTS | PR00242 | Dopamine receptor signature | 80 | 90 | 1.9E-5 |
| 7 | g5177.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 93 | 114 | 1.4E-47 |
| 9 | g5177.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 138 | 160 | 1.4E-47 |
| 13 | g5177.t1 | PRINTS | PR00242 | Dopamine receptor signature | 159 | 167 | 1.9E-5 |
| 10 | g5177.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 174 | 195 | 1.4E-47 |
| 8 | g5177.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 226 | 249 | 1.4E-47 |
| 6 | g5177.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 305 | 329 | 1.4E-47 |
| 14 | g5177.t1 | PRINTS | PR00242 | Dopamine receptor signature | 337 | 348 | 1.9E-5 |
| 4 | g5177.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 342 | 368 | 1.4E-47 |
| 11 | g5177.t1 | PRINTS | PR00242 | Dopamine receptor signature | 362 | 376 | 1.9E-5 |
| 1 | g5177.t1 | Pfam | PF00001 | 7 transmembrane receptor (rhodopsin family) | 75 | 360 | 5.8E-69 |
| 24 | g5177.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 57 | - |
| 27 | g5177.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 58 | 84 | - |
| 17 | g5177.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 85 | 95 | - |
| 31 | g5177.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 96 | 121 | - |
| 21 | g5177.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 122 | 132 | - |
| 26 | g5177.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 133 | 154 | - |
| 18 | g5177.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 155 | 173 | - |
| 28 | g5177.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 174 | 196 | - |
| 22 | g5177.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 197 | 224 | - |
| 25 | g5177.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 225 | 250 | - |
| 20 | g5177.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 251 | 304 | - |
| 30 | g5177.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 305 | 332 | - |
| 23 | g5177.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 333 | 343 | - |
| 29 | g5177.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 344 | 363 | - |
| 19 | g5177.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 364 | 423 | - |
| 41 | g5177.t1 | ProSitePatterns | PS00237 | G-protein coupled receptors family 1 signature. | 144 | 160 | - |
| 42 | g5177.t1 | ProSiteProfiles | PS50262 | G-protein coupled receptors family 1 profile. | 75 | 360 | 42.398 |
| 40 | g5177.t1 | SMART | SM01381 | 7TM_GPCR_Srsx_2 | 69 | 375 | 1.2E-5 |
| 15 | g5177.t1 | SUPERFAMILY | SSF81321 | Family A G protein-coupled receptor-like | 22 | 388 | 1.1E-72 |
| 38 | g5177.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 60 | 82 | - |
| 35 | g5177.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 95 | 117 | - |
| 36 | g5177.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 132 | 154 | - |
| 37 | g5177.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 174 | 196 | - |
| 39 | g5177.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 224 | 246 | - |
| 34 | g5177.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 309 | 331 | - |
| 33 | g5177.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 341 | 363 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5177/g5177.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5177.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007186 | G protein-coupled receptor signaling pathway | BP |
| GO:0016021 | integral component of membrane | CC |
| GO:0004930 | G protein-coupled receptor activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04144 (dopamine receptor D1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed