General information for g5194.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as WD repeat-containing protein 75.
Parent gene
- The parent gene g5194 is located on chr_2: 7609963..7611055 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5194 | g5194.t2 | TTS | g5194.t2 | 7604722 | 7604722 |
| chr_2 | g5194 | g5194.t2 | isoform | g5194.t2 | 7604825 | 7611055 |
| chr_2 | g5194 | g5194.t2 | exon | g5194.t2.exon1 | 7604825 | 7605450 |
| chr_2 | g5194 | g5194.t2 | cds | g5194.t2.CDS1 | 7604825 | 7605450 |
| chr_2 | g5194 | g5194.t2 | exon | g5194.t2.exon2 | 7605505 | 7605562 |
| chr_2 | g5194 | g5194.t2 | cds | g5194.t2.CDS2 | 7605505 | 7605562 |
| chr_2 | g5194 | g5194.t2 | exon | g5194.t2.exon3 | 7605638 | 7605657 |
| chr_2 | g5194 | g5194.t2 | cds | g5194.t2.CDS3 | 7605638 | 7605657 |
| chr_2 | g5194 | g5194.t2 | exon | g5194.t2.exon4 | 7606112 | 7606346 |
| chr_2 | g5194 | g5194.t2 | cds | g5194.t2.CDS4 | 7606112 | 7606346 |
| chr_2 | g5194 | g5194.t2 | exon | g5194.t2.exon5 | 7607973 | 7609452 |
| chr_2 | g5194 | g5194.t2 | cds | g5194.t2.CDS5 | 7607973 | 7609452 |
| chr_2 | g5194 | g5194.t2 | exon | g5194.t2.exon6 | 7609511 | 7610511 |
| chr_2 | g5194 | g5194.t2 | cds | g5194.t2.CDS6 | 7609511 | 7610511 |
| chr_2 | g5194 | g5194.t2 | exon | g5194.t2.exon7 | 7610566 | 7610773 |
| chr_2 | g5194 | g5194.t2 | cds | g5194.t2.CDS7 | 7610566 | 7610773 |
| chr_2 | g5194 | g5194.t2 | exon | g5194.t2.exon8 | 7610862 | 7611055 |
| chr_2 | g5194 | g5194.t2 | cds | g5194.t2.CDS8 | 7610862 | 7611055 |
| chr_2 | g5194 | g5194.t2 | TSS | g5194.t2 | NA | NA |
Sequences
>g5194.t2 Gene=g5194 Length=3822
ATGTGTTTAGACATTGATTATCATTACAACAATCAAAATAGCATAATAAATACGAATTCT
TTGGAAAATCACAATCAAAATATTGACAACAATTCAATCACGATGGAATCAACAACAGAT
TTGAAATATAAAGTGAAAAGTATGTGCGGTGGAAGTGTCATTGAGCATAAACCATTATTT
GATTCAAGTGGAGAAAATATTTATGTAGTGCGAAAAGACAAATTAAGAATCTATAGTGTT
GAAACGGGTGAAATTGTTACAGAATTAGATGACAATAAAGATGGCCAAATAATTGGAATT
TATTTAGAAGATAATCAAACAAATCAATGTATAATAACTTGCACAACAAATGGAACGATT
GCCTTTCGAAAGTTGAAATCGAATGTTATTACAGAAAAGAAGAAATTGAATTTTAAGTTC
TCTATATTAAACAAATTTTTGGTGACAACAATCGATGGCAAATTTCATGGTCTTATTCAC
TATAATGATGAGAAAAATTTTTCACAACTTACACTGATTGAGCTGAGCACTAATAAAATC
GTTCATCGCTTTGATACACCGTTTTTAACTAATCAAGCAGATGTGAAAATGAAGTTTGCA
GATGGTAATGGCATAATTGCAATCATATCAAAAACACATCTCTTTATTATTAATAAAGAG
ACATTAGAAATAATTATGCATAGTGCTCCAAAACTATTGAGTGTTGTTGTCTGTCATCCA
GAACAAAAGATTATTGCAACTGGCGATGTTTATGGAAAAATATATTTATGGAGTAATGTT
TTTAATAAAATGCCAGTAAAAACTGATTTGCATTGGCATCACATGGTTGTGCTAAGTCTT
GCATTTTCTCAATCAGGAACAGTGCTTTATTCTGGTGGTGCTGAATGCGTTTTAGTGAAA
TGGCATATTAGAGAAACAACTTTAGGAAAGAACTTTTTACCTCGCGTTTCTGGTGGAATA
AAACAAATCAGTGTTGATACATTACATGATAAAATTGCAATTTGCATGGATGATAATTCT
ATTCAGATTATAAATTCAAATTTAACTCAATTGAAAACTATTCAAGATTTCACTCAAATT
TCACCATATGACTTGGGATTAAATCAACCATTTCCAGCTGGTATTTGCATCAATCCAAAA
AATAATCATTTAGTCATGAATGGTAAAATTGGTCATTTGCAATTCTTCTCAACAAAAACT
ATGAGACTTTTATTCAATATCGATATCACGCTGCAAAACGTCATTCCGCGTCAAAGAGAA
TTTAATCAATTTTCAACAGAAGTCACTAAAGTAGCTTTCTCAGCATGTGGAATGTGGATG
GCAACTTATGAATGCTGGAATGATAGGATTCATTCACTTGATTCTCGTATAAAGTTTTGG
AGCTTTGATAACATTAAACAAACATATTCACTTCACACTCAAATTGAATATCCTCATGAG
AAAAAAGTTGTAGCAATGCAATTTGCAAATATAGAGAAATCAATAATTTGTGCTAGTGCT
GGTTTAGATCGAGTTGTAAAAATTTGGTCACTTGAAGCATCAGAAGAAATTCAAAATCCA
AAAATGATTTGGATGTTAATTGAACAGATAAATTACAAAAACCTTCCTGTAAAGTGTCTA
AGTTTTTCACAAGATTCATCGTTATTATCAGCAGGATTTGGAAATAGCTTGTGTGTATGG
GACACAACAAATTTTAAATTAAAATGTGCTCTTAGTGCGCCTGCAATAATGGATGGATCT
GTTAATCGTGTTTTGATAACTTTACCAGAGGAAAATTCAAAGAAAAATCTAAACGGAAAT
AAAACAAATTTCATCGAGAAACGACATAAAATTTTGCAAATGATGAATGCAATTATCAAT
GATCCATCTCAAGTGCTTGTTAATAATCTTACACAAGAAAGGAGTAGAATTTTTAAGAGA
AAATTTGACGAAGGAGTAAAGTCACAAGAATTGAAATGCAACGAAAAAAAACTTATTTTT
GATAAAGTAATAGCAACGACTGATTTGAATTTCAATGAAAAACTTCAAATTCTTCACAAA
TTGAACATTTATTACCATATTAGTAATCGTGTAGAAAACGATTTTATAGATTTTATTTCA
AAGAATACATATGATGATATTCATCTTTATAAAGGTCTTCAACAAGGTGTCCTAGAAATC
AAAAACGATGACAAATATAAAATTCTTTGGCGTTTTAAAACTTGGAGAATGCGAGATGTT
AAAAGAAATAGAAAAATCATAACTGTTCGAAAATTACTCAAAAAGCCAATTCGTGAAGAA
GCTTTAAAATTAAAGAAAGCTGAGCCAGGACAAAAGGCGTTACCAATCAAAAATATAAGC
AACATTACGAGCACATTTTTCTGCACAAATGATTTGTCGCATCTATCAGTCGTTACCACA
TCGACTAGACTTTTAATATGGGATTTGCTTACACTTAAAATTCAATCATCTTTCAAAATT
CAGTGTTTGAAATTGGCTCATGATCCTTTGACAAACTTAATTGCAATATTTACAAAACAC
AATGAACTTTTTATCATTCATCCATTACCTGCAATAACTATTTTCCATCAAAAGAATTTG
CCAAATATTCTTGCATTAATTTGGGTACCTCGAGAAATTCCAAAAATGCAATCGTTGAGT
GTCAATTGGCAAGCAACTTCACAACTTTTGTTTTTAAATGAAAACCAAGAAATTTGCACA
TTAAGTTCTCCAACTGATGAAGATGAAACAATTGATTTAACTCCTTACATGAATGAAACA
AACGAAGTCACAGCATCTACAACACCTTTTGCAGCTCTTATATCGAAAAGGCAAAATTAT
AAAGATTCAAATCAAATCACTGTTAGACACATGCTTTCGAATGATTCTGGAAGCATCAAA
GAGATAATCAACTTGTCAAATCATGCTATGCCATCATTATCGAGACTAAACAAGGATTTC
ATTTCATCATTACTGCTGTCACAAAATAAAATTTCGGAAGAAACAGAAACACAGAAATCA
AATAATGATATTGAGACTTATGTTGTATCTGATGATGAAGATGCAGATGCTTATAATTCA
AAATTATCCAAAAGGAAAGCTGTTGAAGTAGGAGAAAAATTACTAAAAGAACGTATAATG
TTTTACTATGAACTTCATTATGTTCTTTATCACATTCATGGATTTTCAATTATTCTGCTA
GTCAACTATATACTTGACACAAGTGTTTTTGCTTCTCCTGAAACAATGACAACAACTCGC
TTCAAAAATGATCCATCAGCAAAAGATTGGAGTTTGTGTGAAAAATGCGAAATAATTGTA
CCGCCTCGAAGCTGGCATTGTGATACATGTAATGTCTGCATTCTAAAACGTGATCATCAC
TGTATGTTTGCTGGAAATTGTATTGGCTATAATAATCATCGACATTTTTTGGTGTTTTTA
CTTTACTTCTTTATTGGAACAACTTATGCATTCTTTTATAATTCATACTATTTGTGGATT
GTTAATGGTTCACTTTTTCTTCAATGGATTACACCTTTGAAAATGATTTTTCCTATGTTT
ATGTTCATGTTTAATACATCAAATGAAATTCATCTTTTATTATACATGCTGATCTTAATT
GGAGCAATTTTTACTGGAGTTTTGATGGTTTATCATGGACGTCTTATAGTTAAAAATGCA
ACAGCACATGAGAAAAACAAAGGCGCATATGATCTCGGCCTTAATAAAAATTTGAAGATT
GTTTTTGGCGACAATTGGCTGTCATGCATTTTATGGCCATTTGCAAAGAGCACATTACCT
GAAATTTACTGGAATGCAGCAGAGAGCCAAAAGAGCAAATAA
>g5194.t2 Gene=g5194 Length=1273
MCLDIDYHYNNQNSIINTNSLENHNQNIDNNSITMESTTDLKYKVKSMCGGSVIEHKPLF
DSSGENIYVVRKDKLRIYSVETGEIVTELDDNKDGQIIGIYLEDNQTNQCIITCTTNGTI
AFRKLKSNVITEKKKLNFKFSILNKFLVTTIDGKFHGLIHYNDEKNFSQLTLIELSTNKI
VHRFDTPFLTNQADVKMKFADGNGIIAIISKTHLFIINKETLEIIMHSAPKLLSVVVCHP
EQKIIATGDVYGKIYLWSNVFNKMPVKTDLHWHHMVVLSLAFSQSGTVLYSGGAECVLVK
WHIRETTLGKNFLPRVSGGIKQISVDTLHDKIAICMDDNSIQIINSNLTQLKTIQDFTQI
SPYDLGLNQPFPAGICINPKNNHLVMNGKIGHLQFFSTKTMRLLFNIDITLQNVIPRQRE
FNQFSTEVTKVAFSACGMWMATYECWNDRIHSLDSRIKFWSFDNIKQTYSLHTQIEYPHE
KKVVAMQFANIEKSIICASAGLDRVVKIWSLEASEEIQNPKMIWMLIEQINYKNLPVKCL
SFSQDSSLLSAGFGNSLCVWDTTNFKLKCALSAPAIMDGSVNRVLITLPEENSKKNLNGN
KTNFIEKRHKILQMMNAIINDPSQVLVNNLTQERSRIFKRKFDEGVKSQELKCNEKKLIF
DKVIATTDLNFNEKLQILHKLNIYYHISNRVENDFIDFISKNTYDDIHLYKGLQQGVLEI
KNDDKYKILWRFKTWRMRDVKRNRKIITVRKLLKKPIREEALKLKKAEPGQKALPIKNIS
NITSTFFCTNDLSHLSVVTTSTRLLIWDLLTLKIQSSFKIQCLKLAHDPLTNLIAIFTKH
NELFIIHPLPAITIFHQKNLPNILALIWVPREIPKMQSLSVNWQATSQLLFLNENQEICT
LSSPTDEDETIDLTPYMNETNEVTASTTPFAALISKRQNYKDSNQITVRHMLSNDSGSIK
EIINLSNHAMPSLSRLNKDFISSLLLSQNKISEETETQKSNNDIETYVVSDDEDADAYNS
KLSKRKAVEVGEKLLKERIMFYYELHYVLYHIHGFSIILLVNYILDTSVFASPETMTTTR
FKNDPSAKDWSLCEKCEIIVPPRSWHCDTCNVCILKRDHHCMFAGNCIGYNNHRHFLVFL
LYFFIGTTYAFFYNSYYLWIVNGSLFLQWITPLKMIFPMFMFMFNTSNEIHLLLYMLILI
GAIFTGVLMVYHGRLIVKNATAHEKNKGAYDLGLNKNLKIVFGDNWLSCILWPFAKSTLP
EIYWNAAESQKSK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g5194.t2 | Gene3D | G3DSA:2.130.10.10 | - | 373 | 601 | 1.2E-16 |
| 2 | g5194.t2 | PANTHER | PTHR44215 | WD REPEAT-CONTAINING PROTEIN 75 | 48 | 1014 | 2.9E-155 |
| 1 | g5194.t2 | Pfam | PF01529 | DHHC palmitoyltransferase | 1090 | 1226 | 3.4E-29 |
| 9 | g5194.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 1135 | - |
| 10 | g5194.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1136 | 1153 | - |
| 7 | g5194.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1154 | 1164 | - |
| 11 | g5194.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1165 | 1184 | - |
| 8 | g5194.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1185 | 1189 | - |
| 12 | g5194.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1190 | 1211 | - |
| 6 | g5194.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1212 | 1273 | - |
| 23 | g5194.t2 | ProSiteProfiles | PS50294 | Trp-Asp (WD) repeats circular profile. | 226 | 311 | 9.23 |
| 24 | g5194.t2 | ProSiteProfiles | PS50294 | Trp-Asp (WD) repeats circular profile. | 452 | 570 | 10.153 |
| 25 | g5194.t2 | ProSiteProfiles | PS50216 | DHHC domain profile. | 1091 | 1141 | 24.047 |
| 19 | g5194.t2 | SMART | SM00320 | WD40_4 | 218 | 258 | 12.0 |
| 18 | g5194.t2 | SMART | SM00320 | WD40_4 | 263 | 302 | 0.99 |
| 20 | g5194.t2 | SMART | SM00320 | WD40_4 | 306 | 345 | 390.0 |
| 22 | g5194.t2 | SMART | SM00320 | WD40_4 | 468 | 510 | 1.0 |
| 21 | g5194.t2 | SMART | SM00320 | WD40_4 | 523 | 561 | 1.1 |
| 17 | g5194.t2 | SMART | SM00320 | WD40_4 | 772 | 808 | 350.0 |
| 3 | g5194.t2 | SUPERFAMILY | SSF50978 | WD40 repeat-like | 60 | 307 | 3.94E-16 |
| 4 | g5194.t2 | SUPERFAMILY | SSF50978 | WD40 repeat-like | 235 | 563 | 5.25E-31 |
| 14 | g5194.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1050 | 1072 | - |
| 13 | g5194.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1138 | 1160 | - |
| 15 | g5194.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1165 | 1184 | - |
| 16 | g5194.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1191 | 1213 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5194/g5194.t2; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5194.t2.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
| GO:0016409 | palmitoyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K14552 (NET1-associated nuclear protein 1 (U3 small nucleolar RNA-associated protein 17))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
