General information for g5198.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dolichyl-phosphate beta-glucosyltransferase.
Parent gene
- The parent gene g5198 is located on chr_2: 7623708..7624310 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5198 | g5198.t1 | isoform | g5198.t1 | 7620444 | 7624310 |
| chr_2 | g5198 | g5198.t1 | exon | g5198.t1.exon1 | 7620444 | 7620495 |
| chr_2 | g5198 | g5198.t1 | cds | g5198.t1.CDS1 | 7620444 | 7620495 |
| chr_2 | g5198 | g5198.t1 | exon | g5198.t1.exon2 | 7620553 | 7623015 |
| chr_2 | g5198 | g5198.t1 | cds | g5198.t1.CDS2 | 7620553 | 7623015 |
| chr_2 | g5198 | g5198.t1 | exon | g5198.t1.exon3 | 7623235 | 7623260 |
| chr_2 | g5198 | g5198.t1 | cds | g5198.t1.CDS3 | 7623235 | 7623260 |
| chr_2 | g5198 | g5198.t1 | exon | g5198.t1.exon4 | 7623341 | 7624181 |
| chr_2 | g5198 | g5198.t1 | cds | g5198.t1.CDS4 | 7623341 | 7624181 |
| chr_2 | g5198 | g5198.t1 | exon | g5198.t1.exon5 | 7624246 | 7624310 |
| chr_2 | g5198 | g5198.t1 | cds | g5198.t1.CDS5 | 7624246 | 7624310 |
| chr_2 | g5198 | g5198.t1 | TTS | g5198.t1 | 7624392 | 7624392 |
| chr_2 | g5198 | g5198.t1 | TSS | g5198.t1 | NA | NA |
Sequences
>g5198.t1 Gene=g5198 Length=3447
ATGATGAGAGTATTATTATTGACAATTTTTATGTTGTGTTACTTTAATTGTGATGTTGGT
AGTGCTGAAATTATCGAAAATACTTGTCTGGAAAAAAGTCGACCACACTCTACAGAATGT
AACAAATATTTCAAATGCATAAAATTGCCAAATAACAGTCTAAAATGGATTACACTGACA
TGTCAAGAGGGACTGATATATGACAGTAATTTGCGTTCATGTGCAATCCCTGATGAAAAT
TGGGACTGTGTATTGAATAGTGCCGAAATGGATGATTATAATAAAGATGAAAATAATATA
TATGGTATTGAAAATTTAAGACTTTTACAAAAAGAAAGTGAGGACAGTGAAGAGGATGAG
AGAGACGGCTATCGAAGTGCAGAAGGTGATGATTTCTTAGAGGTGATTGATGGTGATGAA
ATTTTAAATTCTTCTGGAGAGTCAAGGAGTGAAAATGATGAAAATTCTGCTGATGAAAGT
TCAAATTATAGTGGCGATGGCAGTGTTTTAGATCATGCAGATTACATTACTCAATCGACT
CCACCAAGCATGCAAATGATAACCACTCAAGTGCAAAGATTAACACAATTAATACAAAAT
AATCCGAAAAATAATGCTGACGATTTATCGGCTGATGAGTTAAATTCATATCTCAATGTT
CATAAAATACAAACAAAATCACCAGATTATCAAAATAGTAATTTCCAATCGAACGATAAA
ACACCGATTCCACAAAATGGAAAAATTCATTCTGACATTATGAATGAGATTTTAAGTAAG
CAAAATAAATTGAATTCTATTGGTTTAAAAGAGACAACTCTTGCAATGGATGAAATTACA
GAAAAGCCTGTGATTTATTTTAATAAAGAGCCAATAACTGAAATTCAATTGAAAGGTGGT
CAAGGAATAGATGGTTTAGGTGCTCATCAAATTGTCGTTAATCGACCAGAAGGCGCTGTT
CTTTTCAATGTTCCTTCATCTGACAAGAATAATGTAAAAGAACACACACCTTATATATCT
GAAGACATTTTAAAGACAATTTTGGAACTTTCAAAACAAATGGTGACACAAAATGGCAAT
AATAATCAGAAAAATGCTAACAATAATCAAGAAAATACACGTCCAATTTATTATGCTATT
CCATTTCCAATATATTCTCCACAACCTCACAACAATTTTCAGAATAATTATGATCATTTT
ATGAACAACTCTTTAAGACCTTTAGAAGTTATTACTACCACAACAACAATGCCACTGCCA
GCACCTGTTATGAATATGAATATTATTAGAAGAAAACCAGTCAAGAAAAAGTTGAAAAAT
AAAGTCGATTTCATGGGTCAACAAAATTTAAGAAAGCCTCAGGATAATTTCAATCAATTA
CAATTACAGCAACATCAACAACCATTAGATTATTATTCATATTATAATAATTATCAACAA
AATTATCCTTCATATGGTCCATTAAATTATTATCAACAATATCAATCCGAAAATGACAGA
TATACGAATAATCTCAATCAAAATTTGTATAACAATCGTCTCGGCAATTTTTATGGAAAT
AGTTTTTATGAAAATAGACCAATCGTGAGTGACTCATCAGCATCTATAGCAAACCCATAC
ACAAATCAATTGTATTCTACACCTTATAGAAAAGAAAGTTTATCACCATATAACAATAAC
GACGAATATGATGCATTGCATGAAGAAAGTGATGAGAGCGAAAATAATGTTGGTCTAAGT
GGAAGTTTTAGCGATCATTTCTTTTATGAAGATGAACCTTCACCTCCAAAAGATCCAACC
TTTGGTCTAATTTGTTCATTTGGAACAACAAGACAAGCAAATTTGACAGATTGCACAAAA
TATTATGTGTGTAATTCTAAGACTAAAGAAGTAACTTCTTATTCTTGTCCATTTTCAACA
GCATTTAATGATCAAACAAAATATTGTGACAAAAATTCATTTACAAAATGCAAGGAAATA
AAAGATCGTGAACAAGGAAAATTAAATAATAAGAAATATTATCAACAAGCTCATAAAGCA
CTTCAACAAGCGAAACTAGAATCAGAGAAAATTGAAAGATTGGCTAATATGGTAAGAAAG
GAATCTCAGAAAATTTTACAGAATAGAGCACCATCATCAGCACAACAAAATGATGATGCA
TATCAAGATGAACAAGATGAAGAAGAAATGCTGCCTCAACAAGAAATTTATGCGCCTCCT
CCAATTCGTCAAAGGTCAACTTTACCTCCTGTTCGAATTCTCTCACGTCCTTCAAAACGT
CCACAAATCAAACGAAAGGTCTCAAAAATCTCAAACACTTCAAAATCATCTTCCAATAAA
CGTAGAAAGAAGAAAAAGGTAAAATGCAGTGCAATTGGAAATATTGCAGATCCTGAAGAT
ATGACTTCATACTGGCATTGTTTCAAAAGTCCAGATAATAGAATGAAAAGAATACATAAA
AAATGTAGCTCAAATCTCATTTTCTGTGACACAACACGATATTGTTCATCATCATTGCTT
TTTGTGCTTATATTCATAACACTATGTATTATTTTAAAAGTATCAGCACAACCATTTCCA
ATCATAAAACGACACAAACAAGAGGAGTATTTCTTTGATCCTGTGTCAAATGAGAAACAT
TTATTCTCATCAATTGATGATGAGCCAACACTCGAATTGAGTGTGATAATTCCAGCATAT
GATGAAGAAAAACGATTACCAGTGATGTTAGAAGAATGCCTTGATTATTTAGAGCAAAAA
TGCAAAGAAAATTCCAAATTCTCATATGAAATAATTGTCGTTTCGGATGGTAGTAAAGAT
AAAACAGTTGAAGTTGCACTACAATATTCAAAGAAATTATCATGTGAGAAATTTCGTGTA
CTTGAACTGATTGAAAATCGTGGAAAAGGTGGTGCTGTTCGACTTGGAATGCTTAGTAGT
AGAGGAAAATACTTGCTCTTTGCTGATGCTGATGGTGCAACAAAATTTTCTGATTATACA
TTACTTGAGAAAAGTATAGCAAAATTGTCTAATGGTTGGAAACAGGAAGCAATCTCAATT
GGATCACGTGCTCATCTTGAAAAAGAATCTACAGCTAAGCGCAGCATCTTTAGAACTTTT
TTAATGCATGGTTTTCATATGCTTGTGTGGTTCTTTGCAGTCAAAGAAATCAAAGACACA
CAATGTGGCTTCAAAGTGCTAACACGAAGTGCAGCGAGAATTGTTTTCTCTAATATGCAT
GTTGAAAAATGGGCTTTTGACGTTGAATTGTTATACATAGCACAATCATTAAAAATGCCA
ATAGAAGAAATTGCTGTAAATTGGACTGAAATTGAAGGTTCAAAAATTACTCCTTTCTTT
AGTTGGATCCAAATGGGTAGAGATCTCGTATTAATTTGGTTAAGATATCAAATTGGAGCC
TGGAAGCTGAAAGCTAAATCAAATTAG
>g5198.t1 Gene=g5198 Length=1148
MMRVLLLTIFMLCYFNCDVGSAEIIENTCLEKSRPHSTECNKYFKCIKLPNNSLKWITLT
CQEGLIYDSNLRSCAIPDENWDCVLNSAEMDDYNKDENNIYGIENLRLLQKESEDSEEDE
RDGYRSAEGDDFLEVIDGDEILNSSGESRSENDENSADESSNYSGDGSVLDHADYITQST
PPSMQMITTQVQRLTQLIQNNPKNNADDLSADELNSYLNVHKIQTKSPDYQNSNFQSNDK
TPIPQNGKIHSDIMNEILSKQNKLNSIGLKETTLAMDEITEKPVIYFNKEPITEIQLKGG
QGIDGLGAHQIVVNRPEGAVLFNVPSSDKNNVKEHTPYISEDILKTILELSKQMVTQNGN
NNQKNANNNQENTRPIYYAIPFPIYSPQPHNNFQNNYDHFMNNSLRPLEVITTTTTMPLP
APVMNMNIIRRKPVKKKLKNKVDFMGQQNLRKPQDNFNQLQLQQHQQPLDYYSYYNNYQQ
NYPSYGPLNYYQQYQSENDRYTNNLNQNLYNNRLGNFYGNSFYENRPIVSDSSASIANPY
TNQLYSTPYRKESLSPYNNNDEYDALHEESDESENNVGLSGSFSDHFFYEDEPSPPKDPT
FGLICSFGTTRQANLTDCTKYYVCNSKTKEVTSYSCPFSTAFNDQTKYCDKNSFTKCKEI
KDREQGKLNNKKYYQQAHKALQQAKLESEKIERLANMVRKESQKILQNRAPSSAQQNDDA
YQDEQDEEEMLPQQEIYAPPPIRQRSTLPPVRILSRPSKRPQIKRKVSKISNTSKSSSNK
RRKKKKVKCSAIGNIADPEDMTSYWHCFKSPDNRMKRIHKKCSSNLIFCDTTRYCSSSLL
FVLIFITLCIILKVSAQPFPIIKRHKQEEYFFDPVSNEKHLFSSIDDEPTLELSVIIPAY
DEEKRLPVMLEECLDYLEQKCKENSKFSYEIIVVSDGSKDKTVEVALQYSKKLSCEKFRV
LELIENRGKGGAVRLGMLSSRGKYLLFADADGATKFSDYTLLEKSIAKLSNGWKQEAISI
GSRAHLEKESTAKRSIFRTFLMHGFHMLVWFFAVKEIKDTQCGFKVLTRSAARIVFSNMH
VEKWAFDVELLYIAQSLKMPIEEIAVNWTEIEGSKITPFFSWIQMGRDLVLIWLRYQIGA
WKLKAKSN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 22 | g5198.t1 | CDD | cd04188 | DPG_synthase | 895 | 1115 | 1.49523E-110 |
| 12 | g5198.t1 | Coils | Coil | Coil | 674 | 701 | - |
| 11 | g5198.t1 | Gene3D | G3DSA:2.170.140.10 | Antimicrobial Protein | 604 | 665 | 1.0E-6 |
| 10 | g5198.t1 | Gene3D | G3DSA:3.90.550.10 | Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A | 868 | 1142 | 2.4E-39 |
| 29 | g5198.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 110 | 130 | - |
| 28 | g5198.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 143 | 169 | - |
| 26 | g5198.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 705 | 789 | - |
| 27 | g5198.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 705 | 719 | - |
| 3 | g5198.t1 | PANTHER | PTHR10859:SF91 | DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE | 839 | 1145 | 2.5E-104 |
| 4 | g5198.t1 | PANTHER | PTHR10859 | GLYCOSYL TRANSFERASE | 839 | 1145 | 2.5E-104 |
| 1 | g5198.t1 | Pfam | PF01607 | Chitin binding Peritrophin-A domain | 35 | 83 | 2.1E-5 |
| 2 | g5198.t1 | Pfam | PF00535 | Glycosyl transferase family 2 | 894 | 997 | 2.1E-21 |
| 16 | g5198.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 17 | g5198.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 18 | g5198.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 21 | g5198.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 22 | - |
| 15 | g5198.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 837 | - |
| 20 | g5198.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 838 | 856 | - |
| 13 | g5198.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 857 | 1034 | - |
| 19 | g5198.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1035 | 1053 | - |
| 14 | g5198.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1054 | 1148 | - |
| 32 | g5198.t1 | ProSiteProfiles | PS50940 | Chitin-binding type-2 domain profile. | 26 | 85 | 10.463 |
| 30 | g5198.t1 | ProSiteProfiles | PS50940 | Chitin-binding type-2 domain profile. | 602 | 659 | 11.275 |
| 31 | g5198.t1 | ProSiteProfiles | PS50940 | Chitin-binding type-2 domain profile. | 786 | 851 | 5.846 |
| 25 | g5198.t1 | SMART | SM00494 | chi_10 | 27 | 85 | 4.5E-4 |
| 23 | g5198.t1 | SMART | SM00494 | chi_10 | 603 | 659 | 1.4E-6 |
| 24 | g5198.t1 | SMART | SM00494 | chi_10 | 787 | 842 | 4.0 |
| 6 | g5198.t1 | SUPERFAMILY | SSF57625 | Invertebrate chitin-binding proteins | 33 | 85 | 4.97E-8 |
| 7 | g5198.t1 | SUPERFAMILY | SSF57625 | Invertebrate chitin-binding proteins | 608 | 659 | 3.14E-8 |
| 5 | g5198.t1 | SUPERFAMILY | SSF53448 | Nucleotide-diphospho-sugar transferases | 892 | 1137 | 6.84E-31 |
| 9 | g5198.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
| 8 | g5198.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5198/g5198.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5198.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008061 | chitin binding | MF |
| GO:0005576 | extracellular region | CC |
KEGG
Orthology
- This transcript has been annotated with K00729 (dolichyl-phosphate beta-glucosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
