General information for g5198.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Dolichyl-phosphate beta-glucosyltransferase.
Parent gene
- The parent gene g5198 is located on chr_2: 7623708..7624310 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5198 | g5198.t2 | isoform | g5198.t2 | 7623015 | 7623708 |
| chr_2 | g5198 | g5198.t2 | exon | g5198.t2.exon1 | 7623015 | 7623260 |
| chr_2 | g5198 | g5198.t2 | cds | g5198.t2.CDS1 | 7623189 | 7623260 |
| chr_2 | g5198 | g5198.t2 | exon | g5198.t2.exon2 | 7623341 | 7623708 |
| chr_2 | g5198 | g5198.t2 | cds | g5198.t2.CDS2 | 7623341 | 7623706 |
| chr_2 | g5198 | g5198.t2 | TTS | g5198.t2 | 7624392 | 7624392 |
| chr_2 | g5198 | g5198.t2 | TSS | g5198.t2 | NA | NA |
Sequences
>g5198.t2 Gene=g5198 Length=614
TGTTAGTAAAATATAACGAATAGAAATAATTTAAAAATTCAACTGCCGTCATGTGCATTC
ACACAATTGCTGCCGTAAATAAATATTAAAAAGTTGACAAGACAAAAAAGTTAAATTATA
GAATTATGAAATAAATACAAGAATATTGAAATAAATTATTTTAAAAAACTAAACATGCTT
TCTATCGAATCCTTATTAAATTTAATTTATTGTGGAATAGTGCTTTTTGTGCTTATATTC
ATAACACTATGTATTATTTTAAAAGTATCAGCACAACCATTTCCAATCATAAAACGACAC
AAACAAGAGGAGTATTTCTTTGATCCTGTGTCAAATGAGAAACATTTATTCTCATCAATT
GATGATGAGCCAACACTCGAATTGAGTGTGATAATTCCAGCATATGATGAAGAAAAACGA
TTACCAGTGATGTTAGAAGAATGCCTTGATTATTTAGAGCAAAAATGCAAAGAAAATTCC
AAATTCTCATATGAAATAATTGTCGTTTCGGATGGTAGTAAAGATAAAACAGTTGAAGTT
GCACTACAATATTCAAAGAAATTATCATGTGAGAAATTTCGTGTACTTGAACTGATTGAA
AATCGTGGAAAAGG
>g5198.t2 Gene=g5198 Length=146
MLSIESLLNLIYCGIVLFVLIFITLCIILKVSAQPFPIIKRHKQEEYFFDPVSNEKHLFS
SIDDEPTLELSVIIPAYDEEKRLPVMLEECLDYLEQKCKENSKFSYEIIVVSDGSKDKTV
EVALQYSKKLSCEKFRVLELIENRGK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g5198.t2 | Gene3D | G3DSA:3.90.550.10 | Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A | 54 | 146 | 2.3E-18 |
| 2 | g5198.t2 | PANTHER | PTHR10859:SF91 | DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE | 16 | 146 | 2.0E-29 |
| 3 | g5198.t2 | PANTHER | PTHR10859 | GLYCOSYL TRANSFERASE | 16 | 146 | 2.0E-29 |
| 1 | g5198.t2 | Pfam | PF00535 | Glycosyl transferase family 2 | 71 | 146 | 1.0E-13 |
| 8 | g5198.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 9 | g5198.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 31 | - |
| 7 | g5198.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 32 | 146 | - |
| 5 | g5198.t2 | SUPERFAMILY | SSF53448 | Nucleotide-diphospho-sugar transferases | 68 | 146 | 5.34E-14 |
| 4 | g5198.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5198/g5198.t2; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5198.t2.fa.iupred3.txt does not exist
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
