General information for g5198.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dolichyl-phosphate beta-glucosyltransferase.
Parent gene
- The parent gene g5198 is located on chr_2: 7623708..7624310 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5198 | g5198.t4 | isoform | g5198.t4 | 7623015 | 7624310 |
| chr_2 | g5198 | g5198.t4 | exon | g5198.t4.exon1 | 7623015 | 7623260 |
| chr_2 | g5198 | g5198.t4 | TSS | g5198.t4 | 7623084 | 7623084 |
| chr_2 | g5198 | g5198.t4 | cds | g5198.t4.CDS1 | 7623189 | 7623260 |
| chr_2 | g5198 | g5198.t4 | exon | g5198.t4.exon2 | 7623341 | 7624185 |
| chr_2 | g5198 | g5198.t4 | cds | g5198.t4.CDS2 | 7623341 | 7624185 |
| chr_2 | g5198 | g5198.t4 | exon | g5198.t4.exon3 | 7624246 | 7624310 |
| chr_2 | g5198 | g5198.t4 | cds | g5198.t4.CDS3 | 7624246 | 7624300 |
| chr_2 | g5198 | g5198.t4 | TTS | g5198.t4 | 7624392 | 7624392 |
Sequences
>g5198.t4 Gene=g5198 Length=1156
TGTTAGTAAAATATAACGAATAGAAATAATTTAAAAATTCAACTGCCGTCATGTGCATTC
ACACAATTGCTGCCGTAAATAAATATTAAAAAGTTGACAAGACAAAAAAGTTAAATTATA
GAATTATGAAATAAATACAAGAATATTGAAATAAATTATTTTAAAAAACTAAACATGCTT
TCTATCGAATCCTTATTAAATTTAATTTATTGTGGAATAGTGCTTTTTGTGCTTATATTC
ATAACACTATGTATTATTTTAAAAGTATCAGCACAACCATTTCCAATCATAAAACGACAC
AAACAAGAGGAGTATTTCTTTGATCCTGTGTCAAATGAGAAACATTTATTCTCATCAATT
GATGATGAGCCAACACTCGAATTGAGTGTGATAATTCCAGCATATGATGAAGAAAAACGA
TTACCAGTGATGTTAGAAGAATGCCTTGATTATTTAGAGCAAAAATGCAAAGAAAATTCC
AAATTCTCATATGAAATAATTGTCGTTTCGGATGGTAGTAAAGATAAAACAGTTGAAGTT
GCACTACAATATTCAAAGAAATTATCATGTGAGAAATTTCGTGTACTTGAACTGATTGAA
AATCGTGGAAAAGGTGGTGCTGTTCGACTTGGAATGCTTAGTAGTAGAGGAAAATACTTG
CTCTTTGCTGATGCTGATGGTGCAACAAAATTTTCTGATTATACATTACTTGAGAAAAGT
ATAGCAAAATTGTCTAATGGTTGGAAACAGGAAGCAATCTCAATTGGATCACGTGCTCAT
CTTGAAAAAGAATCTACAGCTAAGCGCAGCATCTTTAGAACTTTTTTAATGCATGGTTTT
CATATGCTTGTGTGGTTCTTTGCAGTCAAAGAAATCAAAGACACACAATGTGGCTTCAAA
GTGCTAACACGAAGTGCAGCGAGAATTGTTTTCTCTAATATGCATGTTGAAAAATGGGCT
TTTGACGTTGAATTGTTATACATAGCACAATCATTAAAAATGCCAATAGAAGAAATTGCT
GTAAATTGGACTGAAATTGAAGGTTCAAAAATTACTCCTTTCTTTAGTTGGATCCAAATG
GGTAGAGGTGAATCTCGTATTAATTTGGTTAAGATATCAAATTGGAGCCTGGAAGCTGAA
AGCTAAATCAAATTAG
>g5198.t4 Gene=g5198 Length=323
MLSIESLLNLIYCGIVLFVLIFITLCIILKVSAQPFPIIKRHKQEEYFFDPVSNEKHLFS
SIDDEPTLELSVIIPAYDEEKRLPVMLEECLDYLEQKCKENSKFSYEIIVVSDGSKDKTV
EVALQYSKKLSCEKFRVLELIENRGKGGAVRLGMLSSRGKYLLFADADGATKFSDYTLLE
KSIAKLSNGWKQEAISIGSRAHLEKESTAKRSIFRTFLMHGFHMLVWFFAVKEIKDTQCG
FKVLTRSAARIVFSNMHVEKWAFDVELLYIAQSLKMPIEEIAVNWTEIEGSKITPFFSWI
QMGRGESRINLVKISNWSLEAES
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g5198.t4 | CDD | cd04188 | DPG_synthase | 72 | 292 | 4.44285E-116 |
| 6 | g5198.t4 | Gene3D | G3DSA:3.90.550.10 | Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A | 44 | 304 | 2.5E-40 |
| 2 | g5198.t4 | PANTHER | PTHR10859:SF91 | DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE | 16 | 304 | 2.5E-100 |
| 3 | g5198.t4 | PANTHER | PTHR10859 | GLYCOSYL TRANSFERASE | 16 | 304 | 2.5E-100 |
| 1 | g5198.t4 | Pfam | PF00535 | Glycosyl transferase family 2 | 71 | 187 | 2.0E-22 |
| 9 | g5198.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 11 | g5198.t4 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 31 | - |
| 7 | g5198.t4 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 32 | 211 | - |
| 10 | g5198.t4 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 212 | 230 | - |
| 8 | g5198.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 231 | 323 | - |
| 5 | g5198.t4 | SUPERFAMILY | SSF53448 | Nucleotide-diphospho-sugar transferases | 68 | 309 | 2.44E-31 |
| 4 | g5198.t4 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5198/g5198.t4; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5198.t4.fa.iupred3.txt does not exist
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
- This transcript has been annotated with K00729 (dolichyl-phosphate beta-glucosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
